Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17280 and BBR_RS17285 are separated by 281 nucleotides BBR_RS17285 and BBR_RS17290 are separated by 13 nucleotides BBR_RS17290 and BBR_RS17295 are separated by 64 nucleotides BBR_RS17295 and BBR_RS17300 are separated by 83 nucleotides
BBR_RS17280: BBR_RS17280 - glycosyl transferase, at 1,654,671 to 1,655,693
_RS17280
BBR_RS17285: BBR_RS17285 - ECF transporter S component, at 1,655,975 to 1,656,631
_RS17285
BBR_RS17290: BBR_RS17290 - Fused ATP-binding protein and permease of ABC transporter, at 1,656,645 to 1,658,996
_RS17290
BBR_RS17295: BBR_RS17295 - RNA-binding protein, at 1,659,061 to 1,659,366
_RS17295
BBR_RS17300: BBR_RS17300 - membrane protein, at 1,659,450 to 1,659,815
_RS17300
Position (kb)
1656
1657
1658
1659 Strain fitness (log2 ratio)
-2
-1
0
1 at 1655.699 kb on + strand at 1655.717 kb on - strand at 1655.717 kb on - strand at 1655.717 kb on - strand at 1655.717 kb on - strand at 1655.717 kb on - strand at 1655.816 kb on - strand at 1655.834 kb on + strand at 1655.855 kb on + strand at 1655.934 kb on + strand at 1655.935 kb on + strand at 1655.969 kb on + strand at 1656.633 kb on - strand at 1656.674 kb on + strand at 1656.725 kb on + strand at 1656.725 kb on + strand at 1656.725 kb on + strand at 1656.742 kb on + strand at 1656.968 kb on + strand, within BBR_RS17290 at 1656.968 kb on + strand, within BBR_RS17290 at 1656.968 kb on + strand, within BBR_RS17290 at 1656.968 kb on + strand, within BBR_RS17290 at 1656.968 kb on + strand, within BBR_RS17290 at 1656.976 kb on - strand, within BBR_RS17290 at 1657.256 kb on - strand, within BBR_RS17290 at 1657.271 kb on + strand, within BBR_RS17290 at 1657.286 kb on + strand, within BBR_RS17290 at 1657.286 kb on + strand, within BBR_RS17290 at 1657.286 kb on + strand, within BBR_RS17290 at 1657.357 kb on + strand, within BBR_RS17290 at 1657.357 kb on + strand, within BBR_RS17290 at 1657.357 kb on + strand, within BBR_RS17290 at 1657.418 kb on + strand, within BBR_RS17290 at 1657.538 kb on + strand, within BBR_RS17290 at 1657.607 kb on + strand, within BBR_RS17290 at 1657.624 kb on - strand, within BBR_RS17290 at 1657.698 kb on + strand, within BBR_RS17290 at 1657.811 kb on - strand, within BBR_RS17290 at 1657.823 kb on - strand, within BBR_RS17290 at 1657.972 kb on + strand, within BBR_RS17290 at 1657.985 kb on + strand, within BBR_RS17290 at 1658.043 kb on - strand, within BBR_RS17290 at 1658.061 kb on - strand, within BBR_RS17290 at 1658.096 kb on - strand, within BBR_RS17290 at 1658.181 kb on - strand, within BBR_RS17290 at 1658.321 kb on + strand, within BBR_RS17290 at 1658.321 kb on + strand, within BBR_RS17290 at 1658.321 kb on + strand, within BBR_RS17290 at 1658.329 kb on - strand, within BBR_RS17290 at 1658.402 kb on + strand, within BBR_RS17290 at 1658.487 kb on - strand, within BBR_RS17290 at 1658.515 kb on + strand, within BBR_RS17290 at 1658.517 kb on - strand, within BBR_RS17290 at 1658.640 kb on + strand, within BBR_RS17290 at 1658.641 kb on - strand, within BBR_RS17290 at 1658.658 kb on - strand, within BBR_RS17290 at 1658.664 kb on + strand, within BBR_RS17290 at 1658.855 kb on - strand at 1658.884 kb on + strand at 1658.884 kb on + strand at 1658.884 kb on + strand at 1658.884 kb on + strand at 1658.884 kb on + strand at 1658.884 kb on + strand at 1658.892 kb on - strand at 1658.892 kb on - strand at 1658.892 kb on - strand at 1658.903 kb on + strand at 1658.955 kb on + strand at 1658.980 kb on + strand at 1658.980 kb on + strand at 1658.980 kb on + strand at 1658.990 kb on + strand at 1658.998 kb on - strand at 1659.032 kb on + strand at 1659.123 kb on + strand, within BBR_RS17295 at 1659.206 kb on + strand, within BBR_RS17295 at 1659.216 kb on + strand, within BBR_RS17295 at 1659.216 kb on + strand, within BBR_RS17295 at 1659.221 kb on + strand, within BBR_RS17295 at 1659.221 kb on + strand, within BBR_RS17295 at 1659.221 kb on + strand, within BBR_RS17295 at 1659.233 kb on + strand, within BBR_RS17295 at 1659.243 kb on - strand, within BBR_RS17295 at 1659.273 kb on - strand, within BBR_RS17295 at 1659.320 kb on - strand, within BBR_RS17295 at 1659.320 kb on - strand, within BBR_RS17295 at 1659.320 kb on - strand, within BBR_RS17295 at 1659.361 kb on + strand at 1659.368 kb on + strand at 1659.379 kb on + strand at 1659.395 kb on + strand at 1659.395 kb on + strand at 1659.445 kb on + strand at 1659.490 kb on + strand, within BBR_RS17300 at 1659.494 kb on + strand, within BBR_RS17300 at 1659.497 kb on - strand, within BBR_RS17300 at 1659.498 kb on - strand, within BBR_RS17300 at 1659.498 kb on - strand, within BBR_RS17300 at 1659.504 kb on + strand, within BBR_RS17300 at 1659.526 kb on - strand, within BBR_RS17300 at 1659.526 kb on - strand, within BBR_RS17300 at 1659.527 kb on - strand, within BBR_RS17300 at 1659.544 kb on + strand, within BBR_RS17300 at 1659.544 kb on + strand, within BBR_RS17300 at 1659.547 kb on - strand, within BBR_RS17300 at 1659.612 kb on - strand, within BBR_RS17300 at 1659.727 kb on + strand, within BBR_RS17300 at 1659.876 kb on - strand at 1659.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,655,699 + +0.1 1,655,717 - -0.0 1,655,717 - +0.0 1,655,717 - +0.3 1,655,717 - +1.2 1,655,717 - +0.8 1,655,816 - +0.8 1,655,834 + +0.2 1,655,855 + +0.5 1,655,934 + +0.5 1,655,935 + +0.7 1,655,969 + +0.3 1,656,633 - -0.2 1,656,674 + -0.6 1,656,725 + -0.2 1,656,725 + +0.7 1,656,725 + +0.6 1,656,742 + -0.5 1,656,968 + BBR_RS17290 0.14 +0.1 1,656,968 + BBR_RS17290 0.14 +0.6 1,656,968 + BBR_RS17290 0.14 -0.5 1,656,968 + BBR_RS17290 0.14 -1.8 1,656,968 + BBR_RS17290 0.14 +0.0 1,656,976 - BBR_RS17290 0.14 -1.3 1,657,256 - BBR_RS17290 0.26 -0.7 1,657,271 + BBR_RS17290 0.27 +0.3 1,657,286 + BBR_RS17290 0.27 -0.0 1,657,286 + BBR_RS17290 0.27 -0.8 1,657,286 + BBR_RS17290 0.27 -1.6 1,657,357 + BBR_RS17290 0.30 -1.7 1,657,357 + BBR_RS17290 0.30 +0.1 1,657,357 + BBR_RS17290 0.30 -0.7 1,657,418 + BBR_RS17290 0.33 +1.0 1,657,538 + BBR_RS17290 0.38 -0.3 1,657,607 + BBR_RS17290 0.41 -0.4 1,657,624 - BBR_RS17290 0.42 +0.7 1,657,698 + BBR_RS17290 0.45 -0.1 1,657,811 - BBR_RS17290 0.50 +0.1 1,657,823 - BBR_RS17290 0.50 -0.4 1,657,972 + BBR_RS17290 0.56 -0.9 1,657,985 + BBR_RS17290 0.57 -0.2 1,658,043 - BBR_RS17290 0.59 -1.7 1,658,061 - BBR_RS17290 0.60 +0.5 1,658,096 - BBR_RS17290 0.62 -1.2 1,658,181 - BBR_RS17290 0.65 -1.2 1,658,321 + BBR_RS17290 0.71 -1.4 1,658,321 + BBR_RS17290 0.71 +0.3 1,658,321 + BBR_RS17290 0.71 -0.2 1,658,329 - BBR_RS17290 0.72 +0.6 1,658,402 + BBR_RS17290 0.75 -1.0 1,658,487 - BBR_RS17290 0.78 -0.5 1,658,515 + BBR_RS17290 0.80 +0.4 1,658,517 - BBR_RS17290 0.80 -0.5 1,658,640 + BBR_RS17290 0.85 -0.6 1,658,641 - BBR_RS17290 0.85 +0.0 1,658,658 - BBR_RS17290 0.86 -1.1 1,658,664 + BBR_RS17290 0.86 -1.9 1,658,855 - +0.2 1,658,884 + +0.1 1,658,884 + +0.8 1,658,884 + -0.1 1,658,884 + -0.6 1,658,884 + -0.8 1,658,884 + +0.0 1,658,892 - +1.1 1,658,892 - -0.4 1,658,892 - +0.3 1,658,903 + -0.9 1,658,955 + -0.7 1,658,980 + +0.5 1,658,980 + +0.6 1,658,980 + +0.1 1,658,990 + -0.1 1,658,998 - +0.1 1,659,032 + -0.2 1,659,123 + BBR_RS17295 0.20 +0.7 1,659,206 + BBR_RS17295 0.47 -0.2 1,659,216 + BBR_RS17295 0.51 -1.2 1,659,216 + BBR_RS17295 0.51 -0.3 1,659,221 + BBR_RS17295 0.52 -1.1 1,659,221 + BBR_RS17295 0.52 +0.2 1,659,221 + BBR_RS17295 0.52 -0.2 1,659,233 + BBR_RS17295 0.56 -0.6 1,659,243 - BBR_RS17295 0.59 -1.5 1,659,273 - BBR_RS17295 0.69 -0.2 1,659,320 - BBR_RS17295 0.85 +0.9 1,659,320 - BBR_RS17295 0.85 +0.1 1,659,320 - BBR_RS17295 0.85 +0.2 1,659,361 + -0.9 1,659,368 + +1.4 1,659,379 + +0.3 1,659,395 + +0.3 1,659,395 + -0.7 1,659,445 + +0.9 1,659,490 + BBR_RS17300 0.11 +0.0 1,659,494 + BBR_RS17300 0.12 +0.6 1,659,497 - BBR_RS17300 0.13 +0.5 1,659,498 - BBR_RS17300 0.13 +0.2 1,659,498 - BBR_RS17300 0.13 +0.1 1,659,504 + BBR_RS17300 0.15 -0.5 1,659,526 - BBR_RS17300 0.21 +0.9 1,659,526 - BBR_RS17300 0.21 -0.2 1,659,527 - BBR_RS17300 0.21 +0.1 1,659,544 + BBR_RS17300 0.26 +0.3 1,659,544 + BBR_RS17300 0.26 +0.1 1,659,547 - BBR_RS17300 0.27 -0.4 1,659,612 - BBR_RS17300 0.44 -0.6 1,659,727 + BBR_RS17300 0.76 +0.5 1,659,876 - -0.1 1,659,919 - -0.4
Or see this region's nucleotide sequence