Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16630 and BBR_RS16635 are separated by 308 nucleotides BBR_RS16635 and BBR_RS16640 are separated by 22 nucleotides
BBR_RS16630: BBR_RS16630 - exodeoxyribonuclease III, at 1,499,490 to 1,500,350
_RS16630
BBR_RS16635: BBR_RS16635 - ABC transporter ATP-binding protein, at 1,500,659 to 1,502,668
_RS16635
BBR_RS16640: BBR_RS16640 - ABC transporter permease, at 1,502,691 to 1,503,695
_RS16640
Position (kb)
1500
1501
1502
1503 Strain fitness (log2 ratio)
-2
-1
0
1 at 1499.782 kb on + strand, within BBR_RS16630 at 1499.782 kb on + strand, within BBR_RS16630 at 1499.901 kb on - strand, within BBR_RS16630 at 1499.948 kb on - strand, within BBR_RS16630 at 1500.140 kb on - strand, within BBR_RS16630 at 1500.185 kb on - strand, within BBR_RS16630 at 1500.199 kb on + strand, within BBR_RS16630 at 1500.254 kb on + strand, within BBR_RS16630 at 1500.254 kb on + strand, within BBR_RS16630 at 1500.295 kb on - strand at 1500.456 kb on - strand at 1500.474 kb on - strand at 1500.489 kb on + strand at 1500.490 kb on - strand at 1500.490 kb on - strand at 1500.593 kb on - strand at 1500.619 kb on + strand at 1500.629 kb on + strand at 1500.637 kb on - strand at 1500.637 kb on - strand at 1500.637 kb on - strand at 1500.662 kb on + strand at 1500.696 kb on + strand at 1500.696 kb on + strand at 1500.806 kb on + strand at 1500.965 kb on - strand, within BBR_RS16635 at 1501.331 kb on + strand, within BBR_RS16635 at 1501.575 kb on + strand, within BBR_RS16635 at 1501.919 kb on - strand, within BBR_RS16635 at 1501.984 kb on - strand, within BBR_RS16635 at 1502.285 kb on - strand, within BBR_RS16635 at 1502.464 kb on - strand, within BBR_RS16635 at 1502.678 kb on + strand at 1502.689 kb on - strand at 1502.719 kb on - strand at 1502.774 kb on - strand at 1502.795 kb on - strand, within BBR_RS16640 at 1502.903 kb on + strand, within BBR_RS16640 at 1502.911 kb on - strand, within BBR_RS16640 at 1502.953 kb on - strand, within BBR_RS16640 at 1503.206 kb on - strand, within BBR_RS16640 at 1503.534 kb on - strand, within BBR_RS16640
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,499,782 + BBR_RS16630 0.34 +1.0 1,499,782 + BBR_RS16630 0.34 -0.1 1,499,901 - BBR_RS16630 0.48 +0.3 1,499,948 - BBR_RS16630 0.53 +1.0 1,500,140 - BBR_RS16630 0.75 +0.1 1,500,185 - BBR_RS16630 0.81 +0.5 1,500,199 + BBR_RS16630 0.82 +0.1 1,500,254 + BBR_RS16630 0.89 +0.1 1,500,254 + BBR_RS16630 0.89 +0.0 1,500,295 - -1.0 1,500,456 - -0.9 1,500,474 - -0.0 1,500,489 + -0.2 1,500,490 - +0.8 1,500,490 - +0.9 1,500,593 - +0.5 1,500,619 + -0.3 1,500,629 + -0.8 1,500,637 - -0.5 1,500,637 - +0.6 1,500,637 - -0.1 1,500,662 + +0.7 1,500,696 + -1.0 1,500,696 + +0.8 1,500,806 + -0.5 1,500,965 - BBR_RS16635 0.15 +1.7 1,501,331 + BBR_RS16635 0.33 +1.3 1,501,575 + BBR_RS16635 0.46 +0.3 1,501,919 - BBR_RS16635 0.63 -2.0 1,501,984 - BBR_RS16635 0.66 +1.3 1,502,285 - BBR_RS16635 0.81 -0.5 1,502,464 - BBR_RS16635 0.90 +1.3 1,502,678 + +1.2 1,502,689 - -0.3 1,502,719 - +0.4 1,502,774 - -1.0 1,502,795 - BBR_RS16640 0.10 +1.3 1,502,903 + BBR_RS16640 0.21 +0.7 1,502,911 - BBR_RS16640 0.22 +0.1 1,502,953 - BBR_RS16640 0.26 +0.7 1,503,206 - BBR_RS16640 0.51 -0.0 1,503,534 - BBR_RS16640 0.84 -0.8
Or see this region's nucleotide sequence