Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS16620

Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS16615 and BBR_RS16620 overlap by 11 nucleotidesBBR_RS16620 and BBR_RS16625 are separated by 15 nucleotidesBBR_RS16625 and BBR_RS16630 are separated by 124 nucleotides BBR_RS16615: BBR_RS16615 - class I SAM-dependent RNA methyltransferase, at 1,496,479 to 1,497,738 _RS16615 BBR_RS16620: BBR_RS16620 - DUF3159 domain-containing protein, at 1,497,728 to 1,498,504 _RS16620 BBR_RS16625: BBR_RS16625 - DUF3710 domain-containing protein, at 1,498,520 to 1,499,365 _RS16625 BBR_RS16630: BBR_RS16630 - exodeoxyribonuclease III, at 1,499,490 to 1,500,350 _RS16630 Position (kb) 1497 1498 1499Strain fitness (log2 ratio) -3 -2 -1 0 1at 1496.741 kb on + strand, within BBR_RS16615at 1496.741 kb on + strand, within BBR_RS16615at 1496.741 kb on + strand, within BBR_RS16615at 1496.749 kb on - strand, within BBR_RS16615at 1496.749 kb on - strand, within BBR_RS16615at 1496.759 kb on - strand, within BBR_RS16615at 1496.823 kb on + strand, within BBR_RS16615at 1496.823 kb on + strand, within BBR_RS16615at 1496.850 kb on - strand, within BBR_RS16615at 1496.856 kb on + strand, within BBR_RS16615at 1496.875 kb on - strand, within BBR_RS16615at 1496.917 kb on + strand, within BBR_RS16615at 1496.917 kb on + strand, within BBR_RS16615at 1496.917 kb on + strand, within BBR_RS16615at 1496.936 kb on + strand, within BBR_RS16615at 1496.944 kb on - strand, within BBR_RS16615at 1496.944 kb on - strand, within BBR_RS16615at 1496.969 kb on + strand, within BBR_RS16615at 1497.014 kb on + strand, within BBR_RS16615at 1497.060 kb on + strand, within BBR_RS16615at 1497.075 kb on + strand, within BBR_RS16615at 1497.091 kb on - strand, within BBR_RS16615at 1497.145 kb on + strand, within BBR_RS16615at 1497.155 kb on + strand, within BBR_RS16615at 1497.191 kb on - strand, within BBR_RS16615at 1497.214 kb on - strand, within BBR_RS16615at 1497.264 kb on - strand, within BBR_RS16615at 1497.312 kb on + strand, within BBR_RS16615at 1497.348 kb on - strand, within BBR_RS16615at 1497.412 kb on - strand, within BBR_RS16615at 1497.497 kb on + strand, within BBR_RS16615at 1497.527 kb on + strand, within BBR_RS16615at 1497.535 kb on - strand, within BBR_RS16615at 1497.555 kb on - strand, within BBR_RS16615at 1497.584 kb on + strand, within BBR_RS16615at 1497.584 kb on + strand, within BBR_RS16615at 1497.621 kb on + strandat 1497.629 kb on - strandat 1497.681 kb on - strandat 1497.692 kb on - strandat 1497.697 kb on + strandat 1497.746 kb on - strandat 1497.903 kb on + strand, within BBR_RS16620at 1497.911 kb on - strand, within BBR_RS16620at 1497.925 kb on - strand, within BBR_RS16620at 1497.959 kb on + strand, within BBR_RS16620at 1498.012 kb on - strand, within BBR_RS16620at 1498.012 kb on - strand, within BBR_RS16620at 1498.041 kb on - strand, within BBR_RS16620at 1498.116 kb on - strand, within BBR_RS16620at 1498.193 kb on - strand, within BBR_RS16620at 1498.221 kb on - strand, within BBR_RS16620at 1498.240 kb on - strand, within BBR_RS16620at 1498.267 kb on + strand, within BBR_RS16620at 1498.388 kb on - strand, within BBR_RS16620at 1498.483 kb on - strandat 1498.513 kb on + strandat 1498.596 kb on + strandat 1498.646 kb on - strand, within BBR_RS16625at 1498.727 kb on - strand, within BBR_RS16625at 1498.773 kb on + strand, within BBR_RS16625at 1498.818 kb on - strand, within BBR_RS16625at 1498.947 kb on - strand, within BBR_RS16625at 1498.958 kb on + strand, within BBR_RS16625at 1498.966 kb on - strand, within BBR_RS16625at 1498.966 kb on - strand, within BBR_RS16625at 1498.983 kb on - strand, within BBR_RS16625at 1498.995 kb on - strand, within BBR_RS16625at 1499.068 kb on - strand, within BBR_RS16625at 1499.087 kb on + strand, within BBR_RS16625at 1499.119 kb on + strand, within BBR_RS16625at 1499.187 kb on - strand, within BBR_RS16625at 1499.215 kb on - strand, within BBR_RS16625at 1499.259 kb on + strand, within BBR_RS16625at 1499.267 kb on - strand, within BBR_RS16625at 1499.267 kb on - strand, within BBR_RS16625at 1499.395 kb on - strandat 1499.423 kb on - strandat 1499.500 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
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1,496,741 + BBR_RS16615 0.21 -0.4
1,496,741 + BBR_RS16615 0.21 +0.6
1,496,741 + BBR_RS16615 0.21 +0.7
1,496,749 - BBR_RS16615 0.21 +0.6
1,496,749 - BBR_RS16615 0.21 -0.4
1,496,759 - BBR_RS16615 0.22 +0.9
1,496,823 + BBR_RS16615 0.27 +0.4
1,496,823 + BBR_RS16615 0.27 +0.1
1,496,850 - BBR_RS16615 0.29 -1.7
1,496,856 + BBR_RS16615 0.30 -2.1
1,496,875 - BBR_RS16615 0.31 -1.6
1,496,917 + BBR_RS16615 0.35 -1.0
1,496,917 + BBR_RS16615 0.35 -1.2
1,496,917 + BBR_RS16615 0.35 -1.2
1,496,936 + BBR_RS16615 0.36 -0.3
1,496,944 - BBR_RS16615 0.37 +0.2
1,496,944 - BBR_RS16615 0.37 +0.2
1,496,969 + BBR_RS16615 0.39 -1.1
1,497,014 + BBR_RS16615 0.42 -1.1
1,497,060 + BBR_RS16615 0.46 -0.9
1,497,075 + BBR_RS16615 0.47 -1.6
1,497,091 - BBR_RS16615 0.49 +1.0
1,497,145 + BBR_RS16615 0.53 -0.9
1,497,155 + BBR_RS16615 0.54 -0.3
1,497,191 - BBR_RS16615 0.57 -0.8
1,497,214 - BBR_RS16615 0.58 +0.0
1,497,264 - BBR_RS16615 0.62 +0.6
1,497,312 + BBR_RS16615 0.66 -1.4
1,497,348 - BBR_RS16615 0.69 -0.8
1,497,412 - BBR_RS16615 0.74 +0.7
1,497,497 + BBR_RS16615 0.81 -0.8
1,497,527 + BBR_RS16615 0.83 -0.6
1,497,535 - BBR_RS16615 0.84 -0.6
1,497,555 - BBR_RS16615 0.85 -1.7
1,497,584 + BBR_RS16615 0.88 -0.7
1,497,584 + BBR_RS16615 0.88 -0.3
1,497,621 + -1.6
1,497,629 - -1.0
1,497,681 - -0.4
1,497,692 - -0.5
1,497,697 + -0.1
1,497,746 - +1.1
1,497,903 + BBR_RS16620 0.23 -1.0
1,497,911 - BBR_RS16620 0.24 -0.7
1,497,925 - BBR_RS16620 0.25 -0.4
1,497,959 + BBR_RS16620 0.30 -1.0
1,498,012 - BBR_RS16620 0.37 -0.3
1,498,012 - BBR_RS16620 0.37 +0.0
1,498,041 - BBR_RS16620 0.40 -0.6
1,498,116 - BBR_RS16620 0.50 -0.4
1,498,193 - BBR_RS16620 0.60 -0.5
1,498,221 - BBR_RS16620 0.63 -0.3
1,498,240 - BBR_RS16620 0.66 +0.2
1,498,267 + BBR_RS16620 0.69 -2.2
1,498,388 - BBR_RS16620 0.85 -0.3
1,498,483 - -0.4
1,498,513 + -0.3
1,498,596 + -0.6
1,498,646 - BBR_RS16625 0.15 +0.3
1,498,727 - BBR_RS16625 0.24 +0.2
1,498,773 + BBR_RS16625 0.30 +0.3
1,498,818 - BBR_RS16625 0.35 -0.3
1,498,947 - BBR_RS16625 0.50 -1.9
1,498,958 + BBR_RS16625 0.52 -1.8
1,498,966 - BBR_RS16625 0.53 -1.2
1,498,966 - BBR_RS16625 0.53 -0.7
1,498,983 - BBR_RS16625 0.55 -0.3
1,498,995 - BBR_RS16625 0.56 -1.7
1,499,068 - BBR_RS16625 0.65 -1.4
1,499,087 + BBR_RS16625 0.67 -1.2
1,499,119 + BBR_RS16625 0.71 -2.9
1,499,187 - BBR_RS16625 0.79 -0.5
1,499,215 - BBR_RS16625 0.82 -0.5
1,499,259 + BBR_RS16625 0.87 +0.7
1,499,267 - BBR_RS16625 0.88 +0.6
1,499,267 - BBR_RS16625 0.88 +0.2
1,499,395 - -0.2
1,499,423 - +0.7
1,499,500 + -0.6

Or see this region's nucleotide sequence