Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16545 and BBR_RS16550 are separated by 100 nucleotides BBR_RS16550 and BBR_RS16555 are separated by 177 nucleotides BBR_RS16555 and BBR_RS16560 are separated by 211 nucleotides
BBR_RS16545: BBR_RS16545 - CinA family protein, at 1,478,056 to 1,478,613
_RS16545
BBR_RS16550: BBR_RS16550 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 1,478,714 to 1,479,349
_RS16550
BBR_RS16555: BBR_RS16555 - DNA translocase FtsK, at 1,479,527 to 1,482,382
_RS16555
BBR_RS16560: BBR_RS16560 - hypothetical protein, at 1,482,594 to 1,483,343
_RS16560
Position (kb)
1479
1480
1481
1482
1483 Strain fitness (log2 ratio)
-2
-1
0
1 at 1478.547 kb on + strand, within BBR_RS16545 at 1478.635 kb on - strand at 1478.672 kb on + strand at 1478.680 kb on - strand at 1478.680 kb on - strand at 1478.690 kb on - strand at 1478.690 kb on - strand at 1478.747 kb on - strand at 1479.366 kb on + strand at 1479.399 kb on + strand at 1479.415 kb on - strand at 1479.419 kb on + strand at 1479.483 kb on - strand at 1479.485 kb on + strand at 1479.493 kb on - strand at 1480.526 kb on - strand, within BBR_RS16555 at 1482.403 kb on + strand at 1482.525 kb on - strand at 1482.527 kb on - strand at 1482.527 kb on - strand at 1482.535 kb on + strand at 1482.545 kb on - strand at 1482.556 kb on - strand at 1482.703 kb on - strand, within BBR_RS16560 at 1482.783 kb on + strand, within BBR_RS16560 at 1482.790 kb on + strand, within BBR_RS16560 at 1482.791 kb on - strand, within BBR_RS16560 at 1482.824 kb on - strand, within BBR_RS16560 at 1482.826 kb on + strand, within BBR_RS16560 at 1482.826 kb on - strand, within BBR_RS16560 at 1482.835 kb on + strand, within BBR_RS16560 at 1482.857 kb on + strand, within BBR_RS16560 at 1482.869 kb on + strand, within BBR_RS16560 at 1482.869 kb on + strand, within BBR_RS16560 at 1482.879 kb on - strand, within BBR_RS16560 at 1482.910 kb on - strand, within BBR_RS16560 at 1482.916 kb on - strand, within BBR_RS16560 at 1482.927 kb on - strand, within BBR_RS16560 at 1482.976 kb on - strand, within BBR_RS16560 at 1482.978 kb on + strand, within BBR_RS16560 at 1483.041 kb on + strand, within BBR_RS16560 at 1483.041 kb on + strand, within BBR_RS16560 at 1483.049 kb on - strand, within BBR_RS16560 at 1483.049 kb on - strand, within BBR_RS16560 at 1483.126 kb on + strand, within BBR_RS16560 at 1483.136 kb on + strand, within BBR_RS16560 at 1483.141 kb on - strand, within BBR_RS16560 at 1483.214 kb on - strand, within BBR_RS16560 at 1483.315 kb on - strand at 1483.325 kb on - strand at 1483.348 kb on + strand at 1483.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,478,547 + BBR_RS16545 0.88 +0.5 1,478,635 - +0.4 1,478,672 + +0.6 1,478,680 - -0.3 1,478,680 - +0.4 1,478,690 - -0.2 1,478,690 - -0.3 1,478,747 - -0.0 1,479,366 + -0.1 1,479,399 + +0.5 1,479,415 - -0.7 1,479,419 + -0.2 1,479,483 - -0.2 1,479,485 + -0.2 1,479,493 - +0.1 1,480,526 - BBR_RS16555 0.35 -0.8 1,482,403 + -0.3 1,482,525 - -0.3 1,482,527 - +1.7 1,482,527 - +0.4 1,482,535 + -0.1 1,482,545 - -0.0 1,482,556 - +0.0 1,482,703 - BBR_RS16560 0.15 +0.2 1,482,783 + BBR_RS16560 0.25 -1.3 1,482,790 + BBR_RS16560 0.26 -1.9 1,482,791 - BBR_RS16560 0.26 +0.4 1,482,824 - BBR_RS16560 0.31 +0.2 1,482,826 + BBR_RS16560 0.31 -0.2 1,482,826 - BBR_RS16560 0.31 +1.4 1,482,835 + BBR_RS16560 0.32 -0.9 1,482,857 + BBR_RS16560 0.35 +0.4 1,482,869 + BBR_RS16560 0.37 +0.1 1,482,869 + BBR_RS16560 0.37 -0.1 1,482,879 - BBR_RS16560 0.38 +0.0 1,482,910 - BBR_RS16560 0.42 +0.1 1,482,916 - BBR_RS16560 0.43 +0.4 1,482,927 - BBR_RS16560 0.44 -0.9 1,482,976 - BBR_RS16560 0.51 +1.0 1,482,978 + BBR_RS16560 0.51 +0.1 1,483,041 + BBR_RS16560 0.60 +0.1 1,483,041 + BBR_RS16560 0.60 +0.3 1,483,049 - BBR_RS16560 0.61 -0.9 1,483,049 - BBR_RS16560 0.61 +0.4 1,483,126 + BBR_RS16560 0.71 -0.2 1,483,136 + BBR_RS16560 0.72 +0.0 1,483,141 - BBR_RS16560 0.73 +0.5 1,483,214 - BBR_RS16560 0.83 -1.2 1,483,315 - -0.3 1,483,325 - +0.2 1,483,348 + -0.1 1,483,348 + -0.0
Or see this region's nucleotide sequence