Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16515 and BBR_RS16520 are separated by 162 nucleotides BBR_RS16520 and BBR_RS16525 are separated by 722 nucleotides
BBR_RS16515: BBR_RS16515 - preprotein translocase subunit SecA, at 1,470,621 to 1,473,503
_RS16515
BBR_RS16520: BBR_RS16520 - ribosome-associated translation inhibitor RaiA, at 1,473,666 to 1,474,328
_RS16520
BBR_RS16525: BBR_RS16525 - RecX family transcriptional regulator, at 1,475,051 to 1,475,650
_RS16525
Position (kb) 1473
1474
1475 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1473.513 kb on - strand at 1473.552 kb on - strand at 1473.554 kb on + strand at 1473.721 kb on - strand at 1473.722 kb on - strand at 1473.783 kb on + strand, within BBR_RS16520 at 1473.789 kb on + strand, within BBR_RS16520 at 1473.835 kb on + strand, within BBR_RS16520 at 1473.836 kb on - strand, within BBR_RS16520 at 1473.884 kb on - strand, within BBR_RS16520 at 1473.890 kb on - strand, within BBR_RS16520 at 1473.908 kb on + strand, within BBR_RS16520 at 1473.942 kb on - strand, within BBR_RS16520 at 1473.972 kb on - strand, within BBR_RS16520 at 1474.004 kb on - strand, within BBR_RS16520 at 1474.048 kb on - strand, within BBR_RS16520 at 1474.086 kb on + strand, within BBR_RS16520 at 1474.086 kb on + strand, within BBR_RS16520 at 1474.089 kb on - strand, within BBR_RS16520 at 1474.096 kb on - strand, within BBR_RS16520 at 1474.096 kb on - strand, within BBR_RS16520 at 1474.096 kb on - strand, within BBR_RS16520 at 1474.106 kb on - strand, within BBR_RS16520 at 1474.106 kb on - strand, within BBR_RS16520 at 1474.111 kb on - strand, within BBR_RS16520 at 1474.118 kb on + strand, within BBR_RS16520 at 1474.123 kb on + strand, within BBR_RS16520 at 1474.127 kb on + strand, within BBR_RS16520 at 1474.131 kb on - strand, within BBR_RS16520 at 1474.158 kb on - strand, within BBR_RS16520 at 1474.158 kb on - strand, within BBR_RS16520 at 1474.182 kb on - strand, within BBR_RS16520 at 1474.234 kb on - strand, within BBR_RS16520 at 1474.252 kb on + strand, within BBR_RS16520 at 1474.405 kb on - strand at 1474.405 kb on - strand at 1474.475 kb on + strand at 1474.506 kb on + strand at 1474.577 kb on + strand at 1475.023 kb on + strand at 1475.093 kb on + strand at 1475.121 kb on - strand, within BBR_RS16525 at 1475.221 kb on - strand, within BBR_RS16525 at 1475.229 kb on + strand, within BBR_RS16525 at 1475.234 kb on + strand, within BBR_RS16525 at 1475.237 kb on - strand, within BBR_RS16525 at 1475.242 kb on - strand, within BBR_RS16525 at 1475.287 kb on - strand, within BBR_RS16525 at 1475.318 kb on + strand, within BBR_RS16525 at 1475.318 kb on + strand, within BBR_RS16525 at 1475.318 kb on + strand, within BBR_RS16525
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,473,513 - -1.6 1,473,552 - -1.3 1,473,554 + -2.1 1,473,721 - -1.2 1,473,722 - -3.4 1,473,783 + BBR_RS16520 0.18 -1.7 1,473,789 + BBR_RS16520 0.19 +0.1 1,473,835 + BBR_RS16520 0.25 +0.3 1,473,836 - BBR_RS16520 0.26 -1.9 1,473,884 - BBR_RS16520 0.33 -0.9 1,473,890 - BBR_RS16520 0.34 -1.0 1,473,908 + BBR_RS16520 0.37 -0.4 1,473,942 - BBR_RS16520 0.42 -0.9 1,473,972 - BBR_RS16520 0.46 -0.3 1,474,004 - BBR_RS16520 0.51 -0.9 1,474,048 - BBR_RS16520 0.58 -0.7 1,474,086 + BBR_RS16520 0.63 +0.6 1,474,086 + BBR_RS16520 0.63 -0.2 1,474,089 - BBR_RS16520 0.64 -0.7 1,474,096 - BBR_RS16520 0.65 -0.0 1,474,096 - BBR_RS16520 0.65 -0.6 1,474,096 - BBR_RS16520 0.65 -0.7 1,474,106 - BBR_RS16520 0.66 -1.1 1,474,106 - BBR_RS16520 0.66 -0.5 1,474,111 - BBR_RS16520 0.67 -1.3 1,474,118 + BBR_RS16520 0.68 -1.3 1,474,123 + BBR_RS16520 0.69 -1.5 1,474,127 + BBR_RS16520 0.70 -1.1 1,474,131 - BBR_RS16520 0.70 -1.4 1,474,158 - BBR_RS16520 0.74 -0.8 1,474,158 - BBR_RS16520 0.74 -0.6 1,474,182 - BBR_RS16520 0.78 -0.0 1,474,234 - BBR_RS16520 0.86 -2.2 1,474,252 + BBR_RS16520 0.88 -0.9 1,474,405 - +0.8 1,474,405 - +0.4 1,474,475 + -0.4 1,474,506 + +0.6 1,474,577 + +0.8 1,475,023 + -0.2 1,475,093 + +0.1 1,475,121 - BBR_RS16525 0.12 +0.2 1,475,221 - BBR_RS16525 0.28 +0.2 1,475,229 + BBR_RS16525 0.30 -0.7 1,475,234 + BBR_RS16525 0.30 -0.4 1,475,237 - BBR_RS16525 0.31 +0.8 1,475,242 - BBR_RS16525 0.32 -0.7 1,475,287 - BBR_RS16525 0.39 -0.1 1,475,318 + BBR_RS16525 0.45 -2.1 1,475,318 + BBR_RS16525 0.45 +0.0 1,475,318 + BBR_RS16525 0.45 +0.1
Or see this region's nucleotide sequence