Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16010 and BBR_RS16015 are separated by 331 nucleotides BBR_RS16015 and BBR_RS16020 are separated by 2 nucleotides BBR_RS16020 and BBR_RS16025 are separated by 140 nucleotides BBR_RS16025 and BBR_RS16030 are separated by 421 nucleotides
BBR_RS16010: BBR_RS16010 - hypothetical protein, at 1,348,221 to 1,348,754
_RS16010
BBR_RS16015: BBR_RS16015 - DUF2089 domain-containing protein, at 1,349,086 to 1,349,388
_RS16015
BBR_RS16020: BBR_RS16020 - hypothetical protein, at 1,349,391 to 1,349,735
_RS16020
BBR_RS16025: BBR_RS16025 - hypothetical protein, at 1,349,876 to 1,350,289
_RS16025
BBR_RS16030: BBR_RS16030 - hypothetical protein, at 1,350,711 to 1,351,352
_RS16030
Position (kb)
1349
1350 Strain fitness (log2 ratio)
-1
0
1 at 1348.427 kb on - strand, within BBR_RS16010 at 1348.429 kb on - strand, within BBR_RS16010 at 1348.485 kb on + strand, within BBR_RS16010 at 1348.485 kb on + strand, within BBR_RS16010 at 1348.491 kb on - strand, within BBR_RS16010 at 1348.516 kb on - strand, within BBR_RS16010 at 1348.537 kb on + strand, within BBR_RS16010 at 1348.692 kb on + strand, within BBR_RS16010 at 1348.718 kb on + strand at 1348.756 kb on - strand at 1348.783 kb on - strand at 1348.877 kb on + strand at 1348.885 kb on - strand at 1348.937 kb on + strand at 1348.991 kb on - strand at 1349.174 kb on + strand, within BBR_RS16015 at 1349.174 kb on + strand, within BBR_RS16015 at 1349.174 kb on + strand, within BBR_RS16015 at 1349.182 kb on - strand, within BBR_RS16015 at 1349.245 kb on - strand, within BBR_RS16015 at 1349.286 kb on - strand, within BBR_RS16015 at 1349.295 kb on + strand, within BBR_RS16015 at 1349.312 kb on - strand, within BBR_RS16015 at 1349.344 kb on + strand, within BBR_RS16015 at 1349.375 kb on + strand at 1349.381 kb on - strand at 1349.387 kb on + strand at 1349.387 kb on + strand at 1349.391 kb on - strand at 1349.476 kb on - strand, within BBR_RS16020 at 1349.491 kb on - strand, within BBR_RS16020 at 1349.564 kb on - strand, within BBR_RS16020 at 1349.599 kb on + strand, within BBR_RS16020 at 1349.756 kb on + strand at 1349.770 kb on - strand at 1349.780 kb on - strand at 1349.897 kb on + strand at 1349.923 kb on + strand, within BBR_RS16025 at 1349.980 kb on - strand, within BBR_RS16025 at 1349.982 kb on + strand, within BBR_RS16025 at 1350.009 kb on + strand, within BBR_RS16025 at 1350.021 kb on + strand, within BBR_RS16025 at 1350.062 kb on - strand, within BBR_RS16025 at 1350.120 kb on + strand, within BBR_RS16025 at 1350.120 kb on + strand, within BBR_RS16025 at 1350.128 kb on - strand, within BBR_RS16025 at 1350.128 kb on - strand, within BBR_RS16025 at 1350.128 kb on - strand, within BBR_RS16025 at 1350.176 kb on + strand, within BBR_RS16025 at 1350.179 kb on - strand, within BBR_RS16025 at 1350.185 kb on + strand, within BBR_RS16025 at 1350.207 kb on - strand, within BBR_RS16025 at 1350.230 kb on - strand, within BBR_RS16025 at 1350.312 kb on + strand at 1350.365 kb on - strand at 1350.365 kb on - strand at 1350.365 kb on - strand at 1350.372 kb on - strand at 1350.392 kb on + strand at 1350.443 kb on - strand at 1350.452 kb on + strand at 1350.452 kb on + strand at 1350.460 kb on - strand at 1350.513 kb on + strand at 1350.532 kb on + strand at 1350.558 kb on + strand at 1350.647 kb on - strand at 1350.647 kb on - strand at 1350.657 kb on + strand at 1350.662 kb on + strand at 1350.670 kb on - strand at 1350.701 kb on + strand at 1350.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,348,427 - BBR_RS16010 0.39 -1.0 1,348,429 - BBR_RS16010 0.39 +0.5 1,348,485 + BBR_RS16010 0.49 +0.8 1,348,485 + BBR_RS16010 0.49 +0.0 1,348,491 - BBR_RS16010 0.51 +0.1 1,348,516 - BBR_RS16010 0.55 +0.5 1,348,537 + BBR_RS16010 0.59 +0.5 1,348,692 + BBR_RS16010 0.88 +0.5 1,348,718 + -0.0 1,348,756 - +0.2 1,348,783 - -1.0 1,348,877 + -0.6 1,348,885 - -0.9 1,348,937 + +0.4 1,348,991 - +0.5 1,349,174 + BBR_RS16015 0.29 +1.0 1,349,174 + BBR_RS16015 0.29 -0.1 1,349,174 + BBR_RS16015 0.29 +1.0 1,349,182 - BBR_RS16015 0.32 +0.1 1,349,245 - BBR_RS16015 0.52 +1.3 1,349,286 - BBR_RS16015 0.66 -0.5 1,349,295 + BBR_RS16015 0.69 +0.5 1,349,312 - BBR_RS16015 0.75 +1.7 1,349,344 + BBR_RS16015 0.85 +0.2 1,349,375 + +1.0 1,349,381 - -1.1 1,349,387 + -0.2 1,349,387 + +0.1 1,349,391 - -0.0 1,349,476 - BBR_RS16020 0.25 -0.5 1,349,491 - BBR_RS16020 0.29 +0.1 1,349,564 - BBR_RS16020 0.50 -0.5 1,349,599 + BBR_RS16020 0.60 -0.2 1,349,756 + +0.4 1,349,770 - -0.7 1,349,780 - -0.3 1,349,897 + -0.0 1,349,923 + BBR_RS16025 0.11 +0.5 1,349,980 - BBR_RS16025 0.25 +0.6 1,349,982 + BBR_RS16025 0.26 +0.4 1,350,009 + BBR_RS16025 0.32 +0.1 1,350,021 + BBR_RS16025 0.35 +0.0 1,350,062 - BBR_RS16025 0.45 +0.1 1,350,120 + BBR_RS16025 0.59 -0.2 1,350,120 + BBR_RS16025 0.59 -0.1 1,350,128 - BBR_RS16025 0.61 -0.3 1,350,128 - BBR_RS16025 0.61 +0.5 1,350,128 - BBR_RS16025 0.61 +0.2 1,350,176 + BBR_RS16025 0.72 -0.2 1,350,179 - BBR_RS16025 0.73 +0.3 1,350,185 + BBR_RS16025 0.75 +0.8 1,350,207 - BBR_RS16025 0.80 -0.4 1,350,230 - BBR_RS16025 0.86 +0.0 1,350,312 + +0.0 1,350,365 - -0.2 1,350,365 - +0.4 1,350,365 - -0.5 1,350,372 - +0.7 1,350,392 + +1.6 1,350,443 - -0.5 1,350,452 + +0.0 1,350,452 + +0.2 1,350,460 - +0.6 1,350,513 + +0.2 1,350,532 + +0.3 1,350,558 + +0.9 1,350,647 - -0.5 1,350,647 - -1.0 1,350,657 + +0.3 1,350,662 + +0.1 1,350,670 - +0.2 1,350,701 + -0.1 1,350,708 - +0.4
Or see this region's nucleotide sequence