Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15850 and BBR_RS15855 are separated by 178 nucleotides BBR_RS15855 and BBR_RS15860 are separated by 157 nucleotides BBR_RS15860 and BBR_RS15865 are separated by 40 nucleotides BBR_RS15865 and BBR_RS15870 are separated by 135 nucleotides
BBR_RS15850: BBR_RS15850 - ASCH domain-containing protein, at 1,321,721 to 1,322,179
_RS15850
BBR_RS15855: BBR_RS15855 - N-acetyltransferase, at 1,322,358 to 1,322,873
_RS15855
BBR_RS15860: BBR_RS15860 - DUF2316 domain-containing protein, at 1,323,031 to 1,323,213
_RS15860
BBR_RS15865: BBR_RS15865 - GNAT family N-acetyltransferase, at 1,323,254 to 1,323,787
_RS15865
BBR_RS15870: BBR_RS15870 - hypothetical protein, at 1,323,923 to 1,324,252
_RS15870
Position (kb)
1323
1324 Strain fitness (log2 ratio)
-1
0
1 at 1322.193 kb on - strand at 1322.193 kb on - strand at 1322.355 kb on + strand at 1322.421 kb on + strand, within BBR_RS15855 at 1322.421 kb on + strand, within BBR_RS15855 at 1322.426 kb on + strand, within BBR_RS15855 at 1322.523 kb on + strand, within BBR_RS15855 at 1322.621 kb on + strand, within BBR_RS15855 at 1322.629 kb on - strand, within BBR_RS15855 at 1322.706 kb on - strand, within BBR_RS15855 at 1322.801 kb on - strand, within BBR_RS15855 at 1322.885 kb on - strand at 1322.964 kb on - strand at 1322.977 kb on + strand at 1323.029 kb on + strand at 1323.037 kb on - strand at 1323.106 kb on + strand, within BBR_RS15860 at 1323.109 kb on + strand, within BBR_RS15860 at 1323.216 kb on - strand at 1323.294 kb on - strand at 1323.302 kb on - strand at 1323.332 kb on + strand, within BBR_RS15865 at 1323.361 kb on + strand, within BBR_RS15865 at 1323.403 kb on - strand, within BBR_RS15865 at 1323.404 kb on - strand, within BBR_RS15865 at 1323.414 kb on - strand, within BBR_RS15865 at 1323.472 kb on - strand, within BBR_RS15865 at 1323.505 kb on + strand, within BBR_RS15865 at 1323.505 kb on + strand, within BBR_RS15865 at 1323.513 kb on - strand, within BBR_RS15865 at 1323.630 kb on - strand, within BBR_RS15865 at 1323.739 kb on + strand at 1323.771 kb on + strand at 1323.806 kb on + strand at 1323.814 kb on - strand at 1323.831 kb on - strand at 1323.852 kb on + strand at 1323.860 kb on - strand at 1323.881 kb on - strand at 1323.911 kb on + strand at 1323.919 kb on - strand at 1323.967 kb on - strand, within BBR_RS15870 at 1323.979 kb on - strand, within BBR_RS15870 at 1323.989 kb on + strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1324.029 kb on + strand, within BBR_RS15870 at 1324.159 kb on + strand, within BBR_RS15870 at 1324.205 kb on - strand, within BBR_RS15870
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,322,193 - -0.0 1,322,193 - +0.9 1,322,355 + -0.2 1,322,421 + BBR_RS15855 0.12 -0.8 1,322,421 + BBR_RS15855 0.12 +1.1 1,322,426 + BBR_RS15855 0.13 +1.0 1,322,523 + BBR_RS15855 0.32 -0.5 1,322,621 + BBR_RS15855 0.51 +0.2 1,322,629 - BBR_RS15855 0.53 -0.2 1,322,706 - BBR_RS15855 0.67 +0.1 1,322,801 - BBR_RS15855 0.86 +0.1 1,322,885 - -1.0 1,322,964 - -0.3 1,322,977 + -0.6 1,323,029 + -0.5 1,323,037 - -0.3 1,323,106 + BBR_RS15860 0.41 +0.7 1,323,109 + BBR_RS15860 0.43 -1.0 1,323,216 - +0.1 1,323,294 - -0.5 1,323,302 - +0.4 1,323,332 + BBR_RS15865 0.15 -0.4 1,323,361 + BBR_RS15865 0.20 -0.6 1,323,403 - BBR_RS15865 0.28 -0.6 1,323,404 - BBR_RS15865 0.28 -0.3 1,323,414 - BBR_RS15865 0.30 +0.3 1,323,472 - BBR_RS15865 0.41 +0.3 1,323,505 + BBR_RS15865 0.47 +1.3 1,323,505 + BBR_RS15865 0.47 -0.0 1,323,513 - BBR_RS15865 0.49 -0.1 1,323,630 - BBR_RS15865 0.70 -1.0 1,323,739 + +0.6 1,323,771 + -0.9 1,323,806 + +0.6 1,323,814 - -1.3 1,323,831 - -0.2 1,323,852 + +1.5 1,323,860 - +0.2 1,323,881 - -0.1 1,323,911 + +0.1 1,323,919 - +0.6 1,323,967 - BBR_RS15870 0.13 +0.5 1,323,979 - BBR_RS15870 0.17 +0.6 1,323,989 + BBR_RS15870 0.20 +0.4 1,323,997 - BBR_RS15870 0.22 -0.2 1,323,997 - BBR_RS15870 0.22 +0.5 1,324,029 + BBR_RS15870 0.32 +0.3 1,324,159 + BBR_RS15870 0.72 +0.0 1,324,205 - BBR_RS15870 0.85 -0.0
Or see this region's nucleotide sequence