Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15435 and BBR_RS15440 are separated by 17 nucleotides BBR_RS15440 and BBR_RS15445 are separated by 151 nucleotides BBR_RS15445 and BBR_RS15450 are separated by 2 nucleotides
BBR_RS15435: BBR_RS15435 - dihydroorotase, at 1,234,140 to 1,235,654
_RS15435
BBR_RS15440: BBR_RS15440 - orotidine-5'-phosphate decarboxylase, at 1,235,672 to 1,236,625
_RS15440
BBR_RS15445: BBR_RS15445 - dihydroorotate dehydrogenase electron transfer subunit, at 1,236,777 to 1,237,601
_RS15445
BBR_RS15450: BBR_RS15450 - dihydroorotate dehydrogenase, at 1,237,604 to 1,238,575
_RS15450
Position (kb)
1235
1236
1237 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1234.923 kb on + strand, within BBR_RS15435 at 1235.122 kb on + strand, within BBR_RS15435 at 1235.130 kb on + strand, within BBR_RS15435 at 1235.130 kb on - strand, within BBR_RS15435 at 1235.285 kb on + strand, within BBR_RS15435 at 1235.330 kb on - strand, within BBR_RS15435 at 1235.478 kb on + strand, within BBR_RS15435 at 1235.486 kb on - strand, within BBR_RS15435 at 1235.521 kb on + strand at 1235.560 kb on - strand at 1235.656 kb on + strand at 1235.658 kb on + strand at 1235.910 kb on + strand, within BBR_RS15440 at 1235.913 kb on + strand, within BBR_RS15440 at 1235.944 kb on + strand, within BBR_RS15440 at 1235.944 kb on + strand, within BBR_RS15440 at 1235.946 kb on + strand, within BBR_RS15440 at 1235.989 kb on - strand, within BBR_RS15440 at 1236.001 kb on + strand, within BBR_RS15440 at 1236.042 kb on + strand, within BBR_RS15440 at 1236.131 kb on - strand, within BBR_RS15440 at 1236.186 kb on + strand, within BBR_RS15440 at 1236.281 kb on + strand, within BBR_RS15440 at 1236.294 kb on - strand, within BBR_RS15440 at 1236.379 kb on + strand, within BBR_RS15440 at 1236.454 kb on + strand, within BBR_RS15440 at 1236.653 kb on + strand at 1236.796 kb on + strand at 1236.917 kb on - strand, within BBR_RS15445 at 1236.923 kb on + strand, within BBR_RS15445 at 1236.937 kb on - strand, within BBR_RS15445 at 1236.956 kb on + strand, within BBR_RS15445 at 1237.007 kb on + strand, within BBR_RS15445 at 1237.007 kb on + strand, within BBR_RS15445 at 1237.030 kb on - strand, within BBR_RS15445 at 1237.062 kb on - strand, within BBR_RS15445 at 1237.187 kb on - strand, within BBR_RS15445 at 1237.192 kb on - strand, within BBR_RS15445 at 1237.192 kb on - strand, within BBR_RS15445 at 1237.194 kb on + strand, within BBR_RS15445 at 1237.281 kb on - strand, within BBR_RS15445 at 1237.308 kb on + strand, within BBR_RS15445 at 1237.405 kb on - strand, within BBR_RS15445 at 1237.426 kb on - strand, within BBR_RS15445 at 1237.426 kb on - strand, within BBR_RS15445 at 1237.496 kb on - strand, within BBR_RS15445 at 1237.529 kb on - strand at 1237.573 kb on + strand at 1237.596 kb on - strand at 1237.606 kb on - strand at 1237.623 kb on - strand at 1237.625 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,234,923 + BBR_RS15435 0.52 -0.2 1,235,122 + BBR_RS15435 0.65 +0.7 1,235,130 + BBR_RS15435 0.65 +2.0 1,235,130 - BBR_RS15435 0.65 +0.3 1,235,285 + BBR_RS15435 0.76 -1.1 1,235,330 - BBR_RS15435 0.79 +1.3 1,235,478 + BBR_RS15435 0.88 +0.7 1,235,486 - BBR_RS15435 0.89 -1.8 1,235,521 + -0.2 1,235,560 - -0.3 1,235,656 + +0.5 1,235,658 + -0.9 1,235,910 + BBR_RS15440 0.25 -0.7 1,235,913 + BBR_RS15440 0.25 -1.4 1,235,944 + BBR_RS15440 0.29 -0.7 1,235,944 + BBR_RS15440 0.29 +0.6 1,235,946 + BBR_RS15440 0.29 -0.3 1,235,989 - BBR_RS15440 0.33 +1.6 1,236,001 + BBR_RS15440 0.34 +1.0 1,236,042 + BBR_RS15440 0.39 -1.4 1,236,131 - BBR_RS15440 0.48 +1.0 1,236,186 + BBR_RS15440 0.54 -1.0 1,236,281 + BBR_RS15440 0.64 -0.8 1,236,294 - BBR_RS15440 0.65 +0.3 1,236,379 + BBR_RS15440 0.74 +0.7 1,236,454 + BBR_RS15440 0.82 -0.4 1,236,653 + +0.2 1,236,796 + +1.2 1,236,917 - BBR_RS15445 0.17 +0.4 1,236,923 + BBR_RS15445 0.18 +0.6 1,236,937 - BBR_RS15445 0.19 -0.3 1,236,956 + BBR_RS15445 0.22 +1.6 1,237,007 + BBR_RS15445 0.28 +0.1 1,237,007 + BBR_RS15445 0.28 -0.8 1,237,030 - BBR_RS15445 0.31 +0.6 1,237,062 - BBR_RS15445 0.35 -0.2 1,237,187 - BBR_RS15445 0.50 +0.1 1,237,192 - BBR_RS15445 0.50 +1.0 1,237,192 - BBR_RS15445 0.50 +0.1 1,237,194 + BBR_RS15445 0.51 -1.1 1,237,281 - BBR_RS15445 0.61 +0.8 1,237,308 + BBR_RS15445 0.64 +0.8 1,237,405 - BBR_RS15445 0.76 +0.3 1,237,426 - BBR_RS15445 0.79 +1.3 1,237,426 - BBR_RS15445 0.79 +0.7 1,237,496 - BBR_RS15445 0.87 -0.5 1,237,529 - +0.2 1,237,573 + +0.4 1,237,596 - -0.5 1,237,606 - +0.2 1,237,623 - +1.6 1,237,625 + -0.1
Or see this region's nucleotide sequence