Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15420 and BBR_RS15425 are separated by 310 nucleotides BBR_RS15425 and BBR_RS15430 overlap by 1 nucleotides BBR_RS15430 and BBR_RS15435 overlap by 4 nucleotides
BBR_RS15420: BBR_RS15420 - bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase, at 1,229,269 to 1,232,451
_RS15420
BBR_RS15425: BBR_RS15425 - aspartate carbamoyltransferase, at 1,232,762 to 1,233,724
_RS15425
BBR_RS15430: BBR_RS15430 - aspartate carbamoyltransferase regulatory subunit, at 1,233,724 to 1,234,143
_RS15430
BBR_RS15435: BBR_RS15435 - dihydroorotase, at 1,234,140 to 1,235,654
_RS15435
Position (kb)
1232
1233
1234 Strain fitness (log2 ratio)
-1
0
1 at 1231.764 kb on - strand, within BBR_RS15420 at 1231.774 kb on + strand, within BBR_RS15420 at 1231.788 kb on - strand, within BBR_RS15420 at 1231.806 kb on - strand, within BBR_RS15420 at 1231.812 kb on + strand, within BBR_RS15420 at 1231.888 kb on + strand, within BBR_RS15420 at 1231.999 kb on + strand, within BBR_RS15420 at 1232.021 kb on - strand, within BBR_RS15420 at 1232.035 kb on - strand, within BBR_RS15420 at 1232.137 kb on + strand at 1232.160 kb on + strand at 1232.166 kb on - strand at 1232.216 kb on + strand at 1232.216 kb on + strand at 1232.307 kb on + strand at 1232.360 kb on - strand at 1232.364 kb on + strand at 1232.428 kb on + strand at 1232.428 kb on + strand at 1232.453 kb on - strand at 1232.462 kb on - strand at 1232.646 kb on + strand at 1232.646 kb on + strand at 1232.882 kb on + strand, within BBR_RS15425 at 1232.923 kb on - strand, within BBR_RS15425 at 1233.009 kb on - strand, within BBR_RS15425 at 1233.156 kb on - strand, within BBR_RS15425 at 1233.156 kb on - strand, within BBR_RS15425 at 1233.333 kb on - strand, within BBR_RS15425 at 1233.535 kb on + strand, within BBR_RS15425 at 1233.622 kb on + strand, within BBR_RS15425 at 1233.702 kb on + strand at 1234.025 kb on + strand, within BBR_RS15430 at 1234.138 kb on - strand at 1234.570 kb on + strand, within BBR_RS15435 at 1234.578 kb on - strand, within BBR_RS15435 at 1234.667 kb on + strand, within BBR_RS15435 at 1234.667 kb on + strand, within BBR_RS15435
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,231,764 - BBR_RS15420 0.78 -0.1 1,231,774 + BBR_RS15420 0.79 +0.0 1,231,788 - BBR_RS15420 0.79 -0.3 1,231,806 - BBR_RS15420 0.80 +0.1 1,231,812 + BBR_RS15420 0.80 +0.2 1,231,888 + BBR_RS15420 0.82 -0.1 1,231,999 + BBR_RS15420 0.86 +0.0 1,232,021 - BBR_RS15420 0.86 -0.0 1,232,035 - BBR_RS15420 0.87 +1.1 1,232,137 + +0.1 1,232,160 + -0.3 1,232,166 - +1.7 1,232,216 + -0.5 1,232,216 + +0.1 1,232,307 + -0.4 1,232,360 - +0.7 1,232,364 + -0.2 1,232,428 + +0.4 1,232,428 + -0.7 1,232,453 - -1.2 1,232,462 - -0.1 1,232,646 + -0.2 1,232,646 + -0.3 1,232,882 + BBR_RS15425 0.12 +0.7 1,232,923 - BBR_RS15425 0.17 -0.0 1,233,009 - BBR_RS15425 0.26 +1.3 1,233,156 - BBR_RS15425 0.41 +0.5 1,233,156 - BBR_RS15425 0.41 -0.0 1,233,333 - BBR_RS15425 0.59 -1.3 1,233,535 + BBR_RS15425 0.80 +1.7 1,233,622 + BBR_RS15425 0.89 -1.0 1,233,702 + -0.1 1,234,025 + BBR_RS15430 0.72 +0.3 1,234,138 - +0.3 1,234,570 + BBR_RS15435 0.28 +0.2 1,234,578 - BBR_RS15435 0.29 -0.0 1,234,667 + BBR_RS15435 0.35 +0.2 1,234,667 + BBR_RS15435 0.35 -0.7
Or see this region's nucleotide sequence