Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15080 and BBR_RS15085 are separated by 34 nucleotides BBR_RS15085 and BBR_RS15090 are separated by 151 nucleotides BBR_RS15090 and BBR_RS20880 are separated by 59 nucleotides BBR_RS20880 and BBR_RS15095 overlap by 36 nucleotides
BBR_RS15080: BBR_RS15080 - chorismate synthase, at 1,151,320 to 1,152,507
_RS15080
BBR_RS15085: BBR_RS15085 - 3-dehydroquinate synthase, at 1,152,542 to 1,154,164
_RS15085
BBR_RS15090: BBR_RS15090 - type II 3-dehydroquinate dehydratase, at 1,154,316 to 1,154,762
_RS15090
BBR_RS20880: BBR_RS20880 - CTP synthetase, at 1,154,822 to 1,154,917
_RS20880
BBR_RS15095: BBR_RS15095 - CTP synthase, at 1,154,882 to 1,156,546
_RS15095
Position (kb)
1152
1153
1154
1155 Strain fitness (log2 ratio)
-2
-1
0
1 at 1151.833 kb on + strand, within BBR_RS15080 at 1151.841 kb on - strand, within BBR_RS15080 at 1151.843 kb on + strand, within BBR_RS15080 at 1152.090 kb on + strand, within BBR_RS15080 at 1152.275 kb on + strand, within BBR_RS15080 at 1152.353 kb on + strand, within BBR_RS15080 at 1152.369 kb on - strand, within BBR_RS15080 at 1152.416 kb on - strand at 1152.464 kb on + strand at 1152.464 kb on + strand at 1152.464 kb on + strand at 1152.522 kb on + strand at 1152.523 kb on - strand at 1152.530 kb on + strand at 1152.530 kb on + strand at 1152.538 kb on - strand at 1152.859 kb on + strand, within BBR_RS15085 at 1152.993 kb on + strand, within BBR_RS15085 at 1153.016 kb on - strand, within BBR_RS15085 at 1154.191 kb on + strand at 1154.193 kb on + strand at 1154.201 kb on - strand at 1154.219 kb on - strand at 1154.228 kb on - strand at 1154.229 kb on - strand at 1154.239 kb on + strand at 1154.273 kb on - strand at 1154.292 kb on - strand at 1154.521 kb on + strand, within BBR_RS15090 at 1154.706 kb on + strand, within BBR_RS15090
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,151,833 + BBR_RS15080 0.43 -1.7 1,151,841 - BBR_RS15080 0.44 -1.3 1,151,843 + BBR_RS15080 0.44 -1.0 1,152,090 + BBR_RS15080 0.65 -1.6 1,152,275 + BBR_RS15080 0.80 -0.3 1,152,353 + BBR_RS15080 0.87 -1.8 1,152,369 - BBR_RS15080 0.88 -1.4 1,152,416 - -1.7 1,152,464 + -2.6 1,152,464 + -1.1 1,152,464 + -0.8 1,152,522 + -1.5 1,152,523 - +0.9 1,152,530 + -0.6 1,152,530 + +0.5 1,152,538 - +0.0 1,152,859 + BBR_RS15085 0.20 -1.0 1,152,993 + BBR_RS15085 0.28 -1.0 1,153,016 - BBR_RS15085 0.29 -0.3 1,154,191 + +0.3 1,154,193 + +0.7 1,154,201 - +0.6 1,154,219 - -0.4 1,154,228 - -0.3 1,154,229 - +0.3 1,154,239 + -0.3 1,154,273 - -0.6 1,154,292 - +0.2 1,154,521 + BBR_RS15090 0.46 -0.5 1,154,706 + BBR_RS15090 0.87 -0.7
Or see this region's nucleotide sequence