Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15070 and BBR_RS15075 are separated by 99 nucleotides BBR_RS15075 and BBR_RS15080 are separated by 48 nucleotides BBR_RS15080 and BBR_RS15085 are separated by 34 nucleotides
BBR_RS15070: BBR_RS15070 - endolytic transglycosylase MltG, at 1,149,511 to 1,150,698
_RS15070
BBR_RS15075: BBR_RS15075 - peptidase A24, at 1,150,798 to 1,151,271
_RS15075
BBR_RS15080: BBR_RS15080 - chorismate synthase, at 1,151,320 to 1,152,507
_RS15080
BBR_RS15085: BBR_RS15085 - 3-dehydroquinate synthase, at 1,152,542 to 1,154,164
_RS15085
Position (kb)
1151
1152
1153 Strain fitness (log2 ratio)
-2
-1
0
1 at 1150.361 kb on - strand, within BBR_RS15070 at 1150.386 kb on + strand, within BBR_RS15070 at 1150.482 kb on + strand, within BBR_RS15070 at 1150.600 kb on + strand at 1150.690 kb on + strand at 1150.698 kb on + strand at 1150.716 kb on + strand at 1150.716 kb on + strand at 1150.719 kb on - strand at 1150.726 kb on + strand at 1150.747 kb on - strand at 1150.755 kb on + strand at 1150.764 kb on - strand at 1150.765 kb on - strand at 1150.790 kb on - strand at 1150.896 kb on + strand, within BBR_RS15075 at 1150.904 kb on - strand, within BBR_RS15075 at 1150.928 kb on + strand, within BBR_RS15075 at 1150.940 kb on + strand, within BBR_RS15075 at 1150.948 kb on - strand, within BBR_RS15075 at 1150.972 kb on - strand, within BBR_RS15075 at 1150.972 kb on - strand, within BBR_RS15075 at 1150.996 kb on + strand, within BBR_RS15075 at 1151.067 kb on + strand, within BBR_RS15075 at 1151.085 kb on - strand, within BBR_RS15075 at 1151.085 kb on - strand, within BBR_RS15075 at 1151.094 kb on - strand, within BBR_RS15075 at 1151.095 kb on - strand, within BBR_RS15075 at 1151.097 kb on - strand, within BBR_RS15075 at 1151.101 kb on + strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.107 kb on - strand, within BBR_RS15075 at 1151.133 kb on + strand, within BBR_RS15075 at 1151.187 kb on - strand, within BBR_RS15075 at 1151.193 kb on + strand, within BBR_RS15075 at 1151.193 kb on + strand, within BBR_RS15075 at 1151.206 kb on - strand, within BBR_RS15075 at 1151.229 kb on - strand at 1151.229 kb on - strand at 1151.234 kb on - strand at 1151.234 kb on - strand at 1151.234 kb on - strand at 1151.262 kb on - strand at 1151.279 kb on + strand at 1151.435 kb on + strand at 1151.833 kb on + strand, within BBR_RS15080 at 1151.841 kb on - strand, within BBR_RS15080 at 1151.843 kb on + strand, within BBR_RS15080 at 1152.090 kb on + strand, within BBR_RS15080 at 1152.275 kb on + strand, within BBR_RS15080 at 1152.353 kb on + strand, within BBR_RS15080 at 1152.369 kb on - strand, within BBR_RS15080 at 1152.416 kb on - strand at 1152.464 kb on + strand at 1152.464 kb on + strand at 1152.464 kb on + strand at 1152.522 kb on + strand at 1152.523 kb on - strand at 1152.530 kb on + strand at 1152.530 kb on + strand at 1152.538 kb on - strand at 1152.859 kb on + strand, within BBR_RS15085 at 1152.993 kb on + strand, within BBR_RS15085 at 1153.016 kb on - strand, within BBR_RS15085
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,150,361 - BBR_RS15070 0.72 -0.2 1,150,386 + BBR_RS15070 0.74 -1.8 1,150,482 + BBR_RS15070 0.82 -0.8 1,150,600 + +0.2 1,150,690 + -0.3 1,150,698 + +0.7 1,150,716 + +1.0 1,150,716 + +0.1 1,150,719 - -0.2 1,150,726 + -0.3 1,150,747 - -0.4 1,150,755 + -0.6 1,150,764 - -0.6 1,150,765 - -0.5 1,150,790 - +0.3 1,150,896 + BBR_RS15075 0.21 -0.5 1,150,904 - BBR_RS15075 0.22 +0.2 1,150,928 + BBR_RS15075 0.27 +0.1 1,150,940 + BBR_RS15075 0.30 -0.5 1,150,948 - BBR_RS15075 0.32 +0.4 1,150,972 - BBR_RS15075 0.37 -0.6 1,150,972 - BBR_RS15075 0.37 +0.4 1,150,996 + BBR_RS15075 0.42 -0.2 1,151,067 + BBR_RS15075 0.57 +0.1 1,151,085 - BBR_RS15075 0.61 -0.5 1,151,085 - BBR_RS15075 0.61 -0.0 1,151,094 - BBR_RS15075 0.62 +0.0 1,151,095 - BBR_RS15075 0.63 +1.0 1,151,097 - BBR_RS15075 0.63 -1.4 1,151,101 + BBR_RS15075 0.64 +0.2 1,151,107 - BBR_RS15075 0.65 -0.2 1,151,107 - BBR_RS15075 0.65 -0.1 1,151,107 - BBR_RS15075 0.65 +0.2 1,151,107 - BBR_RS15075 0.65 +0.4 1,151,133 + BBR_RS15075 0.71 +0.7 1,151,187 - BBR_RS15075 0.82 +0.7 1,151,193 + BBR_RS15075 0.83 -0.5 1,151,193 + BBR_RS15075 0.83 -1.1 1,151,206 - BBR_RS15075 0.86 -0.1 1,151,229 - -0.0 1,151,229 - +0.1 1,151,234 - +0.4 1,151,234 - +0.3 1,151,234 - +0.2 1,151,262 - -0.1 1,151,279 + -0.8 1,151,435 + -1.4 1,151,833 + BBR_RS15080 0.43 -1.7 1,151,841 - BBR_RS15080 0.44 -1.3 1,151,843 + BBR_RS15080 0.44 -1.0 1,152,090 + BBR_RS15080 0.65 -1.6 1,152,275 + BBR_RS15080 0.80 -0.3 1,152,353 + BBR_RS15080 0.87 -1.8 1,152,369 - BBR_RS15080 0.88 -1.4 1,152,416 - -1.7 1,152,464 + -2.6 1,152,464 + -1.1 1,152,464 + -0.8 1,152,522 + -1.5 1,152,523 - +0.9 1,152,530 + -0.6 1,152,530 + +0.5 1,152,538 - +0.0 1,152,859 + BBR_RS15085 0.20 -1.0 1,152,993 + BBR_RS15085 0.28 -1.0 1,153,016 - BBR_RS15085 0.29 -0.3
Or see this region's nucleotide sequence