Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14690 and BBR_RS20845 are separated by 93 nucleotides BBR_RS20845 and BBR_RS14700 are separated by 216 nucleotides BBR_RS14700 and BBR_RS14705 overlap by 14 nucleotides BBR_RS14705 and BBR_RS14710 are separated by 171 nucleotides
BBR_RS14690: BBR_RS14690 - class I SAM-dependent methyltransferase, at 1,071,644 to 1,072,513
_RS14690
BBR_RS20845: BBR_RS20845 - cupin, at 1,072,607 to 1,073,221
_RS20845
BBR_RS14700: BBR_RS14700 - type II toxin-antitoxin system PemK/MazF family toxin, at 1,073,438 to 1,073,761
_RS14700
BBR_RS14705: BBR_RS14705 - translation repressor RelB, at 1,073,748 to 1,073,993
_RS14705
BBR_RS14710: BBR_RS14710 - hypothetical protein, at 1,074,165 to 1,075,421
_RS14710
Position (kb)
1073
1074 Strain fitness (log2 ratio)
-1
0
1
2 at 1072.442 kb on + strand at 1072.445 kb on - strand at 1072.445 kb on - strand at 1072.445 kb on - strand at 1072.445 kb on - strand at 1072.449 kb on + strand at 1072.452 kb on - strand at 1072.485 kb on + strand at 1072.611 kb on - strand at 1072.611 kb on - strand at 1072.632 kb on - strand at 1072.686 kb on - strand, within BBR_RS20845 at 1072.730 kb on - strand, within BBR_RS20845 at 1072.810 kb on - strand, within BBR_RS20845 at 1072.846 kb on - strand, within BBR_RS20845 at 1072.874 kb on + strand, within BBR_RS20845 at 1072.874 kb on + strand, within BBR_RS20845 at 1072.882 kb on - strand, within BBR_RS20845 at 1072.921 kb on + strand, within BBR_RS20845 at 1072.966 kb on - strand, within BBR_RS20845 at 1072.968 kb on + strand, within BBR_RS20845 at 1072.969 kb on - strand, within BBR_RS20845 at 1072.971 kb on - strand, within BBR_RS20845 at 1072.981 kb on - strand, within BBR_RS20845 at 1072.998 kb on - strand, within BBR_RS20845 at 1073.000 kb on + strand, within BBR_RS20845 at 1073.003 kb on - strand, within BBR_RS20845 at 1073.020 kb on + strand, within BBR_RS20845 at 1073.035 kb on - strand, within BBR_RS20845 at 1073.079 kb on - strand, within BBR_RS20845 at 1073.135 kb on + strand, within BBR_RS20845 at 1073.135 kb on + strand, within BBR_RS20845 at 1073.141 kb on - strand, within BBR_RS20845 at 1073.172 kb on - strand at 1073.172 kb on - strand at 1073.182 kb on + strand at 1073.243 kb on + strand at 1073.252 kb on - strand at 1073.270 kb on - strand at 1073.280 kb on - strand at 1073.306 kb on - strand at 1073.347 kb on - strand at 1073.363 kb on - strand at 1073.379 kb on + strand at 1073.379 kb on + strand at 1073.386 kb on - strand at 1073.389 kb on + strand at 1073.408 kb on - strand at 1073.436 kb on + strand at 1073.441 kb on + strand at 1073.544 kb on + strand, within BBR_RS14700 at 1073.669 kb on - strand, within BBR_RS14700 at 1073.679 kb on + strand, within BBR_RS14700 at 1073.687 kb on - strand, within BBR_RS14700 at 1073.693 kb on + strand, within BBR_RS14700 at 1073.747 kb on - strand at 1073.768 kb on + strand at 1073.769 kb on + strand at 1074.044 kb on + strand at 1074.084 kb on + strand at 1074.084 kb on + strand at 1074.084 kb on + strand at 1074.084 kb on + strand at 1074.084 kb on + strand at 1074.089 kb on + strand at 1074.091 kb on + strand at 1074.091 kb on + strand at 1074.092 kb on - strand at 1074.097 kb on - strand at 1074.165 kb on - strand at 1074.180 kb on - strand at 1074.190 kb on + strand at 1074.216 kb on + strand at 1074.361 kb on - strand, within BBR_RS14710 at 1074.405 kb on + strand, within BBR_RS14710 at 1074.413 kb on - strand, within BBR_RS14710 at 1074.413 kb on - strand, within BBR_RS14710 at 1074.443 kb on + strand, within BBR_RS14710 at 1074.451 kb on - strand, within BBR_RS14710 at 1074.451 kb on - strand, within BBR_RS14710 at 1074.451 kb on - strand, within BBR_RS14710 at 1074.505 kb on - strand, within BBR_RS14710 at 1074.512 kb on + strand, within BBR_RS14710 at 1074.519 kb on - strand, within BBR_RS14710 at 1074.521 kb on + strand, within BBR_RS14710 at 1074.565 kb on - strand, within BBR_RS14710 at 1074.567 kb on + strand, within BBR_RS14710 at 1074.567 kb on + strand, within BBR_RS14710 at 1074.575 kb on - strand, within BBR_RS14710 at 1074.632 kb on + strand, within BBR_RS14710 at 1074.632 kb on + strand, within BBR_RS14710 at 1074.751 kb on + strand, within BBR_RS14710
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,072,442 + +0.4 1,072,445 - +0.2 1,072,445 - -0.1 1,072,445 - +0.3 1,072,445 - +0.3 1,072,449 + +1.2 1,072,452 - -0.5 1,072,485 + +0.4 1,072,611 - +0.2 1,072,611 - -0.6 1,072,632 - +0.1 1,072,686 - BBR_RS20845 0.13 +0.3 1,072,730 - BBR_RS20845 0.20 -0.2 1,072,810 - BBR_RS20845 0.33 +0.2 1,072,846 - BBR_RS20845 0.39 +0.9 1,072,874 + BBR_RS20845 0.43 -0.2 1,072,874 + BBR_RS20845 0.43 -0.5 1,072,882 - BBR_RS20845 0.45 +0.8 1,072,921 + BBR_RS20845 0.51 +0.0 1,072,966 - BBR_RS20845 0.58 +0.4 1,072,968 + BBR_RS20845 0.59 +0.1 1,072,969 - BBR_RS20845 0.59 +2.3 1,072,971 - BBR_RS20845 0.59 +0.4 1,072,981 - BBR_RS20845 0.61 -0.2 1,072,998 - BBR_RS20845 0.64 +0.7 1,073,000 + BBR_RS20845 0.64 -0.2 1,073,003 - BBR_RS20845 0.64 +0.0 1,073,020 + BBR_RS20845 0.67 +0.2 1,073,035 - BBR_RS20845 0.70 +0.1 1,073,079 - BBR_RS20845 0.77 +0.5 1,073,135 + BBR_RS20845 0.86 +0.5 1,073,135 + BBR_RS20845 0.86 +0.6 1,073,141 - BBR_RS20845 0.87 +0.5 1,073,172 - +0.1 1,073,172 - +1.0 1,073,182 + +0.5 1,073,243 + +0.6 1,073,252 - +1.1 1,073,270 - +0.1 1,073,280 - -1.2 1,073,306 - +0.5 1,073,347 - +0.3 1,073,363 - +1.2 1,073,379 + -0.9 1,073,379 + +0.3 1,073,386 - +0.4 1,073,389 + +0.4 1,073,408 - +0.7 1,073,436 + +1.1 1,073,441 + +0.6 1,073,544 + BBR_RS14700 0.33 -1.5 1,073,669 - BBR_RS14700 0.71 +0.4 1,073,679 + BBR_RS14700 0.74 -0.0 1,073,687 - BBR_RS14700 0.77 -1.2 1,073,693 + BBR_RS14700 0.79 -1.1 1,073,747 - -0.2 1,073,768 + +0.2 1,073,769 + +1.1 1,074,044 + -0.1 1,074,084 + -0.2 1,074,084 + +0.3 1,074,084 + +0.1 1,074,084 + +1.9 1,074,084 + -1.0 1,074,089 + -0.9 1,074,091 + -0.6 1,074,091 + -0.1 1,074,092 - +1.1 1,074,097 - -0.3 1,074,165 - -0.4 1,074,180 - +0.1 1,074,190 + -0.1 1,074,216 + +0.8 1,074,361 - BBR_RS14710 0.16 -0.4 1,074,405 + BBR_RS14710 0.19 -0.5 1,074,413 - BBR_RS14710 0.20 -0.4 1,074,413 - BBR_RS14710 0.20 -0.0 1,074,443 + BBR_RS14710 0.22 -0.7 1,074,451 - BBR_RS14710 0.23 -1.0 1,074,451 - BBR_RS14710 0.23 +1.6 1,074,451 - BBR_RS14710 0.23 +1.5 1,074,505 - BBR_RS14710 0.27 -0.2 1,074,512 + BBR_RS14710 0.28 +0.1 1,074,519 - BBR_RS14710 0.28 +0.0 1,074,521 + BBR_RS14710 0.28 +0.4 1,074,565 - BBR_RS14710 0.32 -0.3 1,074,567 + BBR_RS14710 0.32 -1.6 1,074,567 + BBR_RS14710 0.32 +0.6 1,074,575 - BBR_RS14710 0.33 +0.4 1,074,632 + BBR_RS14710 0.37 -0.6 1,074,632 + BBR_RS14710 0.37 +0.6 1,074,751 + BBR_RS14710 0.47 +0.8
Or see this region's nucleotide sequence