Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14475 and BBR_RS14480 are separated by 112 nucleotides BBR_RS14480 and BBR_RS14485 are separated by 181 nucleotides BBR_RS14485 and BBR_RS14490 overlap by 4 nucleotides
BBR_RS14475: BBR_RS14475 - formate C-acetyltransferase, at 1,020,990 to 1,023,365
_RS14475
BBR_RS14480: BBR_RS14480 - pyruvate formate-lyase 1-activating enzyme, at 1,023,478 to 1,024,359
_RS14480
BBR_RS14485: BBR_RS14485 - DUF3000 domain-containing protein, at 1,024,541 to 1,025,173
_RS14485
BBR_RS14490: BBR_RS14490 - ribonuclease D, at 1,025,170 to 1,026,471
_RS14490
Position (kb)
1023
1024
1025 Strain fitness (log2 ratio)
-2
-1
0
1 at 1022.492 kb on - strand, within BBR_RS14475 at 1022.555 kb on - strand, within BBR_RS14475 at 1022.660 kb on - strand, within BBR_RS14475 at 1022.669 kb on + strand, within BBR_RS14475 at 1022.669 kb on + strand, within BBR_RS14475 at 1022.684 kb on + strand, within BBR_RS14475 at 1022.769 kb on + strand, within BBR_RS14475 at 1022.806 kb on + strand, within BBR_RS14475 at 1022.888 kb on + strand, within BBR_RS14475 at 1022.889 kb on + strand, within BBR_RS14475 at 1022.944 kb on - strand, within BBR_RS14475 at 1022.973 kb on + strand, within BBR_RS14475 at 1023.011 kb on + strand, within BBR_RS14475 at 1023.011 kb on + strand, within BBR_RS14475 at 1023.013 kb on + strand, within BBR_RS14475 at 1023.013 kb on + strand, within BBR_RS14475 at 1023.094 kb on - strand, within BBR_RS14475 at 1023.194 kb on + strand at 1023.239 kb on - strand at 1023.302 kb on + strand at 1023.341 kb on + strand at 1023.416 kb on + strand at 1023.416 kb on + strand at 1023.417 kb on - strand at 1023.423 kb on - strand at 1023.435 kb on - strand at 1023.528 kb on + strand at 1023.528 kb on + strand at 1023.528 kb on + strand at 1023.602 kb on - strand, within BBR_RS14480 at 1023.650 kb on + strand, within BBR_RS14480 at 1023.711 kb on - strand, within BBR_RS14480 at 1023.757 kb on - strand, within BBR_RS14480 at 1023.800 kb on - strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.833 kb on + strand, within BBR_RS14480 at 1023.907 kb on + strand, within BBR_RS14480 at 1023.923 kb on - strand, within BBR_RS14480 at 1024.019 kb on + strand, within BBR_RS14480 at 1024.089 kb on + strand, within BBR_RS14480 at 1024.116 kb on - strand, within BBR_RS14480 at 1024.178 kb on - strand, within BBR_RS14480 at 1024.250 kb on + strand, within BBR_RS14480 at 1024.258 kb on - strand, within BBR_RS14480 at 1024.299 kb on - strand at 1024.349 kb on - strand at 1024.361 kb on - strand at 1024.383 kb on - strand at 1024.391 kb on - strand at 1024.437 kb on - strand at 1024.444 kb on + strand at 1024.494 kb on - strand at 1024.569 kb on - strand at 1024.569 kb on - strand at 1024.595 kb on + strand at 1024.605 kb on - strand, within BBR_RS14485 at 1024.617 kb on + strand, within BBR_RS14485 at 1024.617 kb on + strand, within BBR_RS14485 at 1024.617 kb on + strand, within BBR_RS14485 at 1024.692 kb on + strand, within BBR_RS14485 at 1024.699 kb on - strand, within BBR_RS14485 at 1024.726 kb on + strand, within BBR_RS14485 at 1024.838 kb on + strand, within BBR_RS14485 at 1024.905 kb on + strand, within BBR_RS14485 at 1024.994 kb on - strand, within BBR_RS14485 at 1024.999 kb on - strand, within BBR_RS14485 at 1025.071 kb on - strand, within BBR_RS14485 at 1025.071 kb on - strand, within BBR_RS14485 at 1025.078 kb on + strand, within BBR_RS14485 at 1025.088 kb on + strand, within BBR_RS14485 at 1025.096 kb on - strand, within BBR_RS14485 at 1025.096 kb on - strand, within BBR_RS14485 at 1025.147 kb on - strand at 1025.159 kb on + strand at 1025.219 kb on + strand at 1025.222 kb on - strand at 1025.262 kb on + strand at 1025.270 kb on - strand at 1025.298 kb on - strand at 1025.326 kb on - strand, within BBR_RS14490 at 1025.326 kb on - strand, within BBR_RS14490 at 1025.342 kb on + strand, within BBR_RS14490
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,022,492 - BBR_RS14475 0.63 -0.6 1,022,555 - BBR_RS14475 0.66 +0.2 1,022,660 - BBR_RS14475 0.70 -0.8 1,022,669 + BBR_RS14475 0.71 -1.1 1,022,669 + BBR_RS14475 0.71 -0.3 1,022,684 + BBR_RS14475 0.71 -1.7 1,022,769 + BBR_RS14475 0.75 -2.5 1,022,806 + BBR_RS14475 0.76 -0.9 1,022,888 + BBR_RS14475 0.80 -0.5 1,022,889 + BBR_RS14475 0.80 -0.6 1,022,944 - BBR_RS14475 0.82 +0.3 1,022,973 + BBR_RS14475 0.83 -0.2 1,023,011 + BBR_RS14475 0.85 +0.6 1,023,011 + BBR_RS14475 0.85 +0.2 1,023,013 + BBR_RS14475 0.85 +0.5 1,023,013 + BBR_RS14475 0.85 -2.4 1,023,094 - BBR_RS14475 0.89 -0.3 1,023,194 + -0.6 1,023,239 - +0.3 1,023,302 + +0.1 1,023,341 + -0.2 1,023,416 + +0.1 1,023,416 + +1.3 1,023,417 - +0.7 1,023,423 - -0.6 1,023,435 - +0.8 1,023,528 + -0.7 1,023,528 + -0.4 1,023,528 + -0.9 1,023,602 - BBR_RS14480 0.14 -0.1 1,023,650 + BBR_RS14480 0.20 -0.0 1,023,711 - BBR_RS14480 0.26 +0.5 1,023,757 - BBR_RS14480 0.32 -0.9 1,023,800 - BBR_RS14480 0.37 -0.5 1,023,822 + BBR_RS14480 0.39 +0.4 1,023,822 + BBR_RS14480 0.39 -1.3 1,023,822 + BBR_RS14480 0.39 -0.3 1,023,822 + BBR_RS14480 0.39 -0.4 1,023,830 - BBR_RS14480 0.40 -0.3 1,023,830 - BBR_RS14480 0.40 -1.0 1,023,830 - BBR_RS14480 0.40 -0.6 1,023,830 - BBR_RS14480 0.40 -0.7 1,023,833 + BBR_RS14480 0.40 -0.5 1,023,907 + BBR_RS14480 0.49 +0.3 1,023,923 - BBR_RS14480 0.50 +0.2 1,024,019 + BBR_RS14480 0.61 -1.5 1,024,089 + BBR_RS14480 0.69 -0.8 1,024,116 - BBR_RS14480 0.72 +0.2 1,024,178 - BBR_RS14480 0.79 -0.6 1,024,250 + BBR_RS14480 0.88 -0.8 1,024,258 - BBR_RS14480 0.88 -0.9 1,024,299 - +0.0 1,024,349 - +0.2 1,024,361 - -0.6 1,024,383 - -0.1 1,024,391 - -0.3 1,024,437 - -0.2 1,024,444 + +0.2 1,024,494 - -0.1 1,024,569 - -0.1 1,024,569 - -0.1 1,024,595 + +0.1 1,024,605 - BBR_RS14485 0.10 -0.2 1,024,617 + BBR_RS14485 0.12 -1.3 1,024,617 + BBR_RS14485 0.12 +0.3 1,024,617 + BBR_RS14485 0.12 +0.1 1,024,692 + BBR_RS14485 0.24 +0.7 1,024,699 - BBR_RS14485 0.25 +0.5 1,024,726 + BBR_RS14485 0.29 +0.2 1,024,838 + BBR_RS14485 0.47 +0.3 1,024,905 + BBR_RS14485 0.58 +0.3 1,024,994 - BBR_RS14485 0.72 +0.3 1,024,999 - BBR_RS14485 0.72 -0.5 1,025,071 - BBR_RS14485 0.84 +0.2 1,025,071 - BBR_RS14485 0.84 +0.0 1,025,078 + BBR_RS14485 0.85 +0.1 1,025,088 + BBR_RS14485 0.86 -0.1 1,025,096 - BBR_RS14485 0.88 -0.7 1,025,096 - BBR_RS14485 0.88 +0.0 1,025,147 - -0.5 1,025,159 + +0.5 1,025,219 + +0.4 1,025,222 - +0.4 1,025,262 + -0.3 1,025,270 - -0.3 1,025,298 - -0.0 1,025,326 - BBR_RS14490 0.12 -0.1 1,025,326 - BBR_RS14490 0.12 +0.5 1,025,342 + BBR_RS14490 0.13 +0.4
Or see this region's nucleotide sequence