Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14465 and BBR_RS14470 are separated by 19 nucleotides BBR_RS14470 and BBR_RS14475 are separated by 274 nucleotides BBR_RS14475 and BBR_RS14480 are separated by 112 nucleotides
BBR_RS14465: BBR_RS14465 - NAD+ synthase, at 1,018,768 to 1,020,465
_RS14465
BBR_RS14470: BBR_RS14470 - hypothetical protein, at 1,020,485 to 1,020,715
_RS14470
BBR_RS14475: BBR_RS14475 - formate C-acetyltransferase, at 1,020,990 to 1,023,365
_RS14475
BBR_RS14480: BBR_RS14480 - pyruvate formate-lyase 1-activating enzyme, at 1,023,478 to 1,024,359
_RS14480
Position (kb)
1020
1021
1022
1023
1024 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1020.511 kb on + strand, within BBR_RS14470 at 1020.534 kb on + strand, within BBR_RS14470 at 1020.542 kb on - strand, within BBR_RS14470 at 1020.570 kb on + strand, within BBR_RS14470 at 1020.576 kb on - strand, within BBR_RS14470 at 1020.578 kb on - strand, within BBR_RS14470 at 1020.586 kb on + strand, within BBR_RS14470 at 1020.613 kb on + strand, within BBR_RS14470 at 1020.618 kb on - strand, within BBR_RS14470 at 1020.618 kb on - strand, within BBR_RS14470 at 1020.620 kb on + strand, within BBR_RS14470 at 1020.623 kb on - strand, within BBR_RS14470 at 1020.634 kb on + strand, within BBR_RS14470 at 1020.763 kb on + strand at 1020.767 kb on + strand at 1020.775 kb on - strand at 1020.911 kb on + strand at 1020.911 kb on + strand at 1021.051 kb on + strand at 1021.084 kb on - strand at 1021.118 kb on + strand at 1021.125 kb on + strand at 1021.125 kb on + strand at 1021.198 kb on + strand at 1021.245 kb on - strand, within BBR_RS14475 at 1021.294 kb on - strand, within BBR_RS14475 at 1021.470 kb on + strand, within BBR_RS14475 at 1021.484 kb on + strand, within BBR_RS14475 at 1021.509 kb on + strand, within BBR_RS14475 at 1021.567 kb on + strand, within BBR_RS14475 at 1021.567 kb on + strand, within BBR_RS14475 at 1021.572 kb on + strand, within BBR_RS14475 at 1021.608 kb on - strand, within BBR_RS14475 at 1021.612 kb on + strand, within BBR_RS14475 at 1021.621 kb on + strand, within BBR_RS14475 at 1021.700 kb on + strand, within BBR_RS14475 at 1021.744 kb on + strand, within BBR_RS14475 at 1021.749 kb on + strand, within BBR_RS14475 at 1021.764 kb on + strand, within BBR_RS14475 at 1021.847 kb on - strand, within BBR_RS14475 at 1021.847 kb on - strand, within BBR_RS14475 at 1021.900 kb on - strand, within BBR_RS14475 at 1021.926 kb on - strand, within BBR_RS14475 at 1022.080 kb on + strand, within BBR_RS14475 at 1022.090 kb on + strand, within BBR_RS14475 at 1022.104 kb on + strand, within BBR_RS14475 at 1022.114 kb on + strand, within BBR_RS14475 at 1022.130 kb on + strand, within BBR_RS14475 at 1022.138 kb on - strand, within BBR_RS14475 at 1022.250 kb on + strand, within BBR_RS14475 at 1022.339 kb on + strand, within BBR_RS14475 at 1022.344 kb on + strand, within BBR_RS14475 at 1022.346 kb on + strand, within BBR_RS14475 at 1022.359 kb on - strand, within BBR_RS14475 at 1022.359 kb on - strand, within BBR_RS14475 at 1022.397 kb on + strand, within BBR_RS14475 at 1022.416 kb on - strand, within BBR_RS14475 at 1022.445 kb on + strand, within BBR_RS14475 at 1022.492 kb on - strand, within BBR_RS14475 at 1022.555 kb on - strand, within BBR_RS14475 at 1022.660 kb on - strand, within BBR_RS14475 at 1022.669 kb on + strand, within BBR_RS14475 at 1022.669 kb on + strand, within BBR_RS14475 at 1022.684 kb on + strand, within BBR_RS14475 at 1022.769 kb on + strand, within BBR_RS14475 at 1022.806 kb on + strand, within BBR_RS14475 at 1022.888 kb on + strand, within BBR_RS14475 at 1022.889 kb on + strand, within BBR_RS14475 at 1022.944 kb on - strand, within BBR_RS14475 at 1022.973 kb on + strand, within BBR_RS14475 at 1023.011 kb on + strand, within BBR_RS14475 at 1023.011 kb on + strand, within BBR_RS14475 at 1023.013 kb on + strand, within BBR_RS14475 at 1023.013 kb on + strand, within BBR_RS14475 at 1023.094 kb on - strand, within BBR_RS14475 at 1023.194 kb on + strand at 1023.239 kb on - strand at 1023.302 kb on + strand at 1023.341 kb on + strand at 1023.416 kb on + strand at 1023.416 kb on + strand at 1023.417 kb on - strand at 1023.423 kb on - strand at 1023.435 kb on - strand at 1023.528 kb on + strand at 1023.528 kb on + strand at 1023.528 kb on + strand at 1023.602 kb on - strand, within BBR_RS14480 at 1023.650 kb on + strand, within BBR_RS14480 at 1023.711 kb on - strand, within BBR_RS14480 at 1023.757 kb on - strand, within BBR_RS14480 at 1023.800 kb on - strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.822 kb on + strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.830 kb on - strand, within BBR_RS14480 at 1023.833 kb on + strand, within BBR_RS14480 at 1023.907 kb on + strand, within BBR_RS14480 at 1023.923 kb on - strand, within BBR_RS14480 at 1024.019 kb on + strand, within BBR_RS14480 at 1024.089 kb on + strand, within BBR_RS14480 at 1024.116 kb on - strand, within BBR_RS14480 at 1024.178 kb on - strand, within BBR_RS14480 at 1024.250 kb on + strand, within BBR_RS14480 at 1024.258 kb on - strand, within BBR_RS14480 at 1024.299 kb on - strand at 1024.349 kb on - strand at 1024.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,020,511 + BBR_RS14470 0.11 -1.0 1,020,534 + BBR_RS14470 0.21 +0.8 1,020,542 - BBR_RS14470 0.25 +0.8 1,020,570 + BBR_RS14470 0.37 +0.0 1,020,576 - BBR_RS14470 0.39 +0.7 1,020,578 - BBR_RS14470 0.40 +1.1 1,020,586 + BBR_RS14470 0.44 +0.7 1,020,613 + BBR_RS14470 0.55 +0.9 1,020,618 - BBR_RS14470 0.58 -0.6 1,020,618 - BBR_RS14470 0.58 -0.0 1,020,620 + BBR_RS14470 0.58 +0.2 1,020,623 - BBR_RS14470 0.60 +0.7 1,020,634 + BBR_RS14470 0.65 +0.1 1,020,763 + +0.4 1,020,767 + +0.8 1,020,775 - +1.8 1,020,911 + -0.2 1,020,911 + -0.4 1,021,051 + -0.2 1,021,084 - -1.1 1,021,118 + -0.6 1,021,125 + +0.1 1,021,125 + +0.7 1,021,198 + -1.8 1,021,245 - BBR_RS14475 0.11 +0.0 1,021,294 - BBR_RS14475 0.13 -1.0 1,021,470 + BBR_RS14475 0.20 -1.3 1,021,484 + BBR_RS14475 0.21 +0.0 1,021,509 + BBR_RS14475 0.22 -1.1 1,021,567 + BBR_RS14475 0.24 +0.2 1,021,567 + BBR_RS14475 0.24 -0.8 1,021,572 + BBR_RS14475 0.24 -0.8 1,021,608 - BBR_RS14475 0.26 -2.0 1,021,612 + BBR_RS14475 0.26 -0.2 1,021,621 + BBR_RS14475 0.27 -1.1 1,021,700 + BBR_RS14475 0.30 -0.8 1,021,744 + BBR_RS14475 0.32 -0.3 1,021,749 + BBR_RS14475 0.32 -1.2 1,021,764 + BBR_RS14475 0.33 -1.2 1,021,847 - BBR_RS14475 0.36 -0.4 1,021,847 - BBR_RS14475 0.36 +1.2 1,021,900 - BBR_RS14475 0.38 -0.8 1,021,926 - BBR_RS14475 0.39 -1.4 1,022,080 + BBR_RS14475 0.46 +0.9 1,022,090 + BBR_RS14475 0.46 -1.6 1,022,104 + BBR_RS14475 0.47 -1.7 1,022,114 + BBR_RS14475 0.47 +1.3 1,022,130 + BBR_RS14475 0.48 +0.9 1,022,138 - BBR_RS14475 0.48 -0.3 1,022,250 + BBR_RS14475 0.53 -1.2 1,022,339 + BBR_RS14475 0.57 -1.3 1,022,344 + BBR_RS14475 0.57 -1.4 1,022,346 + BBR_RS14475 0.57 -0.3 1,022,359 - BBR_RS14475 0.58 -0.5 1,022,359 - BBR_RS14475 0.58 -0.5 1,022,397 + BBR_RS14475 0.59 -0.8 1,022,416 - BBR_RS14475 0.60 +0.7 1,022,445 + BBR_RS14475 0.61 +0.0 1,022,492 - BBR_RS14475 0.63 -0.6 1,022,555 - BBR_RS14475 0.66 +0.2 1,022,660 - BBR_RS14475 0.70 -0.8 1,022,669 + BBR_RS14475 0.71 -1.1 1,022,669 + BBR_RS14475 0.71 -0.3 1,022,684 + BBR_RS14475 0.71 -1.7 1,022,769 + BBR_RS14475 0.75 -2.5 1,022,806 + BBR_RS14475 0.76 -0.9 1,022,888 + BBR_RS14475 0.80 -0.5 1,022,889 + BBR_RS14475 0.80 -0.6 1,022,944 - BBR_RS14475 0.82 +0.3 1,022,973 + BBR_RS14475 0.83 -0.2 1,023,011 + BBR_RS14475 0.85 +0.6 1,023,011 + BBR_RS14475 0.85 +0.2 1,023,013 + BBR_RS14475 0.85 +0.5 1,023,013 + BBR_RS14475 0.85 -2.4 1,023,094 - BBR_RS14475 0.89 -0.3 1,023,194 + -0.6 1,023,239 - +0.3 1,023,302 + +0.1 1,023,341 + -0.2 1,023,416 + +0.1 1,023,416 + +1.3 1,023,417 - +0.7 1,023,423 - -0.6 1,023,435 - +0.8 1,023,528 + -0.7 1,023,528 + -0.4 1,023,528 + -0.9 1,023,602 - BBR_RS14480 0.14 -0.1 1,023,650 + BBR_RS14480 0.20 -0.0 1,023,711 - BBR_RS14480 0.26 +0.5 1,023,757 - BBR_RS14480 0.32 -0.9 1,023,800 - BBR_RS14480 0.37 -0.5 1,023,822 + BBR_RS14480 0.39 +0.4 1,023,822 + BBR_RS14480 0.39 -1.3 1,023,822 + BBR_RS14480 0.39 -0.3 1,023,822 + BBR_RS14480 0.39 -0.4 1,023,830 - BBR_RS14480 0.40 -0.3 1,023,830 - BBR_RS14480 0.40 -1.0 1,023,830 - BBR_RS14480 0.40 -0.6 1,023,830 - BBR_RS14480 0.40 -0.7 1,023,833 + BBR_RS14480 0.40 -0.5 1,023,907 + BBR_RS14480 0.49 +0.3 1,023,923 - BBR_RS14480 0.50 +0.2 1,024,019 + BBR_RS14480 0.61 -1.5 1,024,089 + BBR_RS14480 0.69 -0.8 1,024,116 - BBR_RS14480 0.72 +0.2 1,024,178 - BBR_RS14480 0.79 -0.6 1,024,250 + BBR_RS14480 0.88 -0.8 1,024,258 - BBR_RS14480 0.88 -0.9 1,024,299 - +0.0 1,024,349 - +0.2 1,024,361 - -0.6
Or see this region's nucleotide sequence