Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14365 and BBR_RS14370 are separated by 27 nucleotides BBR_RS14370 and BBR_RS14375 are separated by 227 nucleotides
BBR_RS14365: BBR_RS14365 - hypothetical protein, at 996,344 to 997,516
_RS14365
BBR_RS14370: BBR_RS14370 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 997,544 to 998,878
_RS14370
BBR_RS14375: BBR_RS14375 - acetate kinase, at 999,106 to 1,000,335
_RS14375
Position (kb) 997
998
999 Strain fitness (log2 ratio) -2
-1
0
1
2 at 996.565 kb on + strand, within BBR_RS14365 at 996.578 kb on - strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.730 kb on - strand, within BBR_RS14365 at 996.742 kb on + strand, within BBR_RS14365 at 996.833 kb on + strand, within BBR_RS14365 at 996.848 kb on + strand, within BBR_RS14365 at 996.928 kb on + strand, within BBR_RS14365 at 996.932 kb on - strand, within BBR_RS14365 at 997.011 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.029 kb on - strand, within BBR_RS14365 at 997.143 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.293 kb on + strand, within BBR_RS14365 at 997.344 kb on - strand, within BBR_RS14365 at 997.346 kb on + strand, within BBR_RS14365 at 997.423 kb on + strand at 997.423 kb on + strand at 997.510 kb on + strand at 997.510 kb on + strand at 997.548 kb on - strand at 997.564 kb on + strand at 997.750 kb on + strand, within BBR_RS14370 at 997.777 kb on - strand, within BBR_RS14370 at 997.779 kb on + strand, within BBR_RS14370 at 997.793 kb on - strand, within BBR_RS14370 at 997.853 kb on + strand, within BBR_RS14370 at 997.861 kb on - strand, within BBR_RS14370 at 997.872 kb on + strand, within BBR_RS14370 at 997.911 kb on + strand, within BBR_RS14370 at 997.954 kb on - strand, within BBR_RS14370 at 997.964 kb on - strand, within BBR_RS14370 at 998.056 kb on + strand, within BBR_RS14370 at 998.150 kb on + strand, within BBR_RS14370 at 998.150 kb on + strand, within BBR_RS14370 at 998.186 kb on + strand, within BBR_RS14370 at 998.187 kb on - strand, within BBR_RS14370 at 998.348 kb on + strand, within BBR_RS14370 at 998.358 kb on + strand, within BBR_RS14370 at 998.363 kb on - strand, within BBR_RS14370 at 998.555 kb on + strand, within BBR_RS14370 at 998.712 kb on + strand, within BBR_RS14370 at 998.718 kb on - strand, within BBR_RS14370 at 998.873 kb on + strand at 998.878 kb on + strand at 998.907 kb on + strand at 998.931 kb on + strand at 998.960 kb on - strand at 998.969 kb on + strand at 998.979 kb on + strand at 998.979 kb on + strand at 999.026 kb on + strand at 999.071 kb on - strand at 999.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 996,565 + BBR_RS14365 0.19 -0.4 996,578 - BBR_RS14365 0.20 +0.0 996,616 + BBR_RS14365 0.23 -0.1 996,616 + BBR_RS14365 0.23 +0.9 996,730 - BBR_RS14365 0.33 +0.2 996,742 + BBR_RS14365 0.34 +0.0 996,833 + BBR_RS14365 0.42 +0.1 996,848 + BBR_RS14365 0.43 -0.1 996,928 + BBR_RS14365 0.50 +1.1 996,932 - BBR_RS14365 0.50 -0.5 997,011 + BBR_RS14365 0.57 -0.2 997,021 + BBR_RS14365 0.58 +1.0 997,021 + BBR_RS14365 0.58 -0.8 997,029 - BBR_RS14365 0.58 -1.2 997,143 - BBR_RS14365 0.68 -0.9 997,150 - BBR_RS14365 0.69 -0.7 997,150 - BBR_RS14365 0.69 -0.9 997,293 + BBR_RS14365 0.81 +2.3 997,344 - BBR_RS14365 0.85 -0.9 997,346 + BBR_RS14365 0.85 -0.1 997,423 + +0.6 997,423 + +0.1 997,510 + -0.4 997,510 + -1.4 997,548 - -0.5 997,564 + -1.1 997,750 + BBR_RS14370 0.15 -0.8 997,777 - BBR_RS14370 0.17 -1.5 997,779 + BBR_RS14370 0.18 +0.7 997,793 - BBR_RS14370 0.19 +2.3 997,853 + BBR_RS14370 0.23 +1.3 997,861 - BBR_RS14370 0.24 +0.2 997,872 + BBR_RS14370 0.25 +2.1 997,911 + BBR_RS14370 0.27 +0.3 997,954 - BBR_RS14370 0.31 -0.3 997,964 - BBR_RS14370 0.31 +0.0 998,056 + BBR_RS14370 0.38 -0.6 998,150 + BBR_RS14370 0.45 -1.1 998,150 + BBR_RS14370 0.45 -0.7 998,186 + BBR_RS14370 0.48 -0.7 998,187 - BBR_RS14370 0.48 +0.5 998,348 + BBR_RS14370 0.60 -2.0 998,358 + BBR_RS14370 0.61 +0.5 998,363 - BBR_RS14370 0.61 +1.0 998,555 + BBR_RS14370 0.76 -0.1 998,712 + BBR_RS14370 0.87 -1.5 998,718 - BBR_RS14370 0.88 +0.1 998,873 + -0.1 998,878 + -0.7 998,907 + +2.3 998,931 + +0.2 998,960 - +1.2 998,969 + -0.3 998,979 + +0.3 998,979 + +0.2 999,026 + -0.6 999,071 - -0.7 999,119 + -0.2
Or see this region's nucleotide sequence