Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14320 and BBR_RS14325 are separated by 42 nucleotides BBR_RS14325 and BBR_RS14330 are separated by 73 nucleotides BBR_RS14330 and BBR_RS14335 are separated by 137 nucleotides BBR_RS14335 and BBR_RS14340 are separated by 148 nucleotides BBR_RS14340 and BBR_RS14345 are separated by 3 nucleotides
BBR_RS14320: BBR_RS14320 - Nif3-like dinuclear metal center hexameric protein, at 989,045 to 989,965
_RS14320
BBR_RS14325: BBR_RS14325 - NUDIX hydrolase, at 990,008 to 990,682
_RS14325
BBR_RS14330: BBR_RS14330 - glycogen debranching enzyme GlgX, at 990,756 to 992,867
_RS14330
BBR_RS14335: BBR_RS14335 - hypothetical protein, at 993,005 to 993,205
_RS14335
BBR_RS14340: BBR_RS14340 - helix-turn-helix domain-containing protein, at 993,354 to 993,839
_RS14340
BBR_RS14345: BBR_RS14345 - hypothetical protein, at 993,843 to 994,493
_RS14345
Position (kb) 990
991
992
993 Strain fitness (log2 ratio) -2
-1
0
1
2 at 989.757 kb on + strand, within BBR_RS14320 at 989.759 kb on - strand, within BBR_RS14320 at 989.791 kb on - strand, within BBR_RS14320 at 989.811 kb on - strand, within BBR_RS14320 at 989.850 kb on + strand, within BBR_RS14320 at 989.850 kb on + strand, within BBR_RS14320 at 989.881 kb on + strand at 989.941 kb on + strand at 989.941 kb on + strand at 989.941 kb on + strand at 989.949 kb on - strand at 989.954 kb on + strand at 989.954 kb on + strand at 989.963 kb on - strand at 989.978 kb on - strand at 989.980 kb on - strand at 990.008 kb on - strand at 990.084 kb on + strand, within BBR_RS14325 at 990.114 kb on + strand, within BBR_RS14325 at 990.114 kb on + strand, within BBR_RS14325 at 990.165 kb on - strand, within BBR_RS14325 at 990.204 kb on + strand, within BBR_RS14325 at 990.260 kb on + strand, within BBR_RS14325 at 990.260 kb on + strand, within BBR_RS14325 at 990.269 kb on - strand, within BBR_RS14325 at 990.275 kb on - strand, within BBR_RS14325 at 990.301 kb on - strand, within BBR_RS14325 at 990.309 kb on + strand, within BBR_RS14325 at 990.372 kb on + strand, within BBR_RS14325 at 990.411 kb on - strand, within BBR_RS14325 at 990.444 kb on + strand, within BBR_RS14325 at 990.475 kb on + strand, within BBR_RS14325 at 990.475 kb on + strand, within BBR_RS14325 at 990.489 kb on + strand, within BBR_RS14325 at 990.561 kb on - strand, within BBR_RS14325 at 990.943 kb on - strand at 990.960 kb on + strand at 991.420 kb on - strand, within BBR_RS14330 at 991.487 kb on + strand, within BBR_RS14330 at 991.825 kb on - strand, within BBR_RS14330 at 991.989 kb on - strand, within BBR_RS14330 at 992.029 kb on + strand, within BBR_RS14330 at 992.037 kb on - strand, within BBR_RS14330 at 992.047 kb on - strand, within BBR_RS14330 at 992.078 kb on + strand, within BBR_RS14330 at 992.284 kb on - strand, within BBR_RS14330 at 992.546 kb on - strand, within BBR_RS14330 at 992.573 kb on + strand, within BBR_RS14330 at 992.678 kb on + strand at 992.788 kb on + strand at 992.910 kb on + strand at 992.917 kb on + strand at 992.917 kb on + strand at 992.956 kb on - strand at 992.962 kb on + strand at 993.000 kb on + strand at 993.101 kb on + strand, within BBR_RS14335 at 993.101 kb on + strand, within BBR_RS14335 at 993.125 kb on - strand, within BBR_RS14335 at 993.132 kb on + strand, within BBR_RS14335 at 993.132 kb on + strand, within BBR_RS14335 at 993.140 kb on - strand, within BBR_RS14335 at 993.181 kb on + strand, within BBR_RS14335 at 993.189 kb on - strand at 993.286 kb on + strand at 993.294 kb on - strand at 993.304 kb on - strand at 993.371 kb on + strand at 993.390 kb on + strand at 993.519 kb on - strand, within BBR_RS14340 at 993.732 kb on + strand, within BBR_RS14340 at 993.742 kb on + strand, within BBR_RS14340 at 993.760 kb on - strand, within BBR_RS14340 at 993.760 kb on - strand, within BBR_RS14340 at 993.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 989,757 + BBR_RS14320 0.77 +0.5 989,759 - BBR_RS14320 0.78 -1.2 989,791 - BBR_RS14320 0.81 +0.1 989,811 - BBR_RS14320 0.83 -0.1 989,850 + BBR_RS14320 0.87 -0.5 989,850 + BBR_RS14320 0.87 -0.0 989,881 + -0.4 989,941 + -2.2 989,941 + -0.0 989,941 + -0.1 989,949 - -0.0 989,954 + -0.1 989,954 + -0.2 989,963 - -0.6 989,978 - -0.5 989,980 - -0.1 990,008 - -0.3 990,084 + BBR_RS14325 0.11 +0.7 990,114 + BBR_RS14325 0.16 -0.4 990,114 + BBR_RS14325 0.16 -1.0 990,165 - BBR_RS14325 0.23 -1.1 990,204 + BBR_RS14325 0.29 -0.7 990,260 + BBR_RS14325 0.37 +0.6 990,260 + BBR_RS14325 0.37 -0.7 990,269 - BBR_RS14325 0.39 -1.0 990,275 - BBR_RS14325 0.40 -0.6 990,301 - BBR_RS14325 0.43 -0.2 990,309 + BBR_RS14325 0.45 -0.0 990,372 + BBR_RS14325 0.54 +0.5 990,411 - BBR_RS14325 0.60 -1.6 990,444 + BBR_RS14325 0.65 +0.5 990,475 + BBR_RS14325 0.69 +0.1 990,475 + BBR_RS14325 0.69 +0.2 990,489 + BBR_RS14325 0.71 +0.0 990,561 - BBR_RS14325 0.82 -0.4 990,943 - +0.7 990,960 + +2.0 991,420 - BBR_RS14330 0.31 +1.3 991,487 + BBR_RS14330 0.35 -0.7 991,825 - BBR_RS14330 0.51 +1.3 991,989 - BBR_RS14330 0.58 +0.3 992,029 + BBR_RS14330 0.60 +1.3 992,037 - BBR_RS14330 0.61 +0.7 992,047 - BBR_RS14330 0.61 +2.3 992,078 + BBR_RS14330 0.63 +0.7 992,284 - BBR_RS14330 0.72 +0.3 992,546 - BBR_RS14330 0.85 +0.3 992,573 + BBR_RS14330 0.86 +0.3 992,678 + -0.3 992,788 + +0.7 992,910 + +0.1 992,917 + -0.5 992,917 + -0.0 992,956 - +0.0 992,962 + +0.3 993,000 + -0.1 993,101 + BBR_RS14335 0.48 -0.7 993,101 + BBR_RS14335 0.48 +0.6 993,125 - BBR_RS14335 0.60 +0.5 993,132 + BBR_RS14335 0.63 -1.3 993,132 + BBR_RS14335 0.63 -0.2 993,140 - BBR_RS14335 0.67 +0.2 993,181 + BBR_RS14335 0.88 +0.0 993,189 - +0.5 993,286 + -0.8 993,294 - -0.1 993,304 - +0.1 993,371 + -0.3 993,390 + +0.3 993,519 - BBR_RS14340 0.34 +0.4 993,732 + BBR_RS14340 0.78 +0.0 993,742 + BBR_RS14340 0.80 -0.4 993,760 - BBR_RS14340 0.84 +0.1 993,760 - BBR_RS14340 0.84 -0.1 993,809 - +0.3
Or see this region's nucleotide sequence