Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14220 and BBR_RS14225 are separated by 71 nucleotides BBR_RS14225 and BBR_RS14230 are separated by 57 nucleotides BBR_RS14230 and BBR_RS14235 overlap by 1 nucleotides BBR_RS14235 and BBR_RS14240 are separated by 30 nucleotides
BBR_RS14220: BBR_RS14220 - phosphomannomutase, at 965,609 to 966,412
_RS14220
BBR_RS14225: BBR_RS14225 - ComF family protein, at 966,484 to 967,158
_RS14225
BBR_RS14230: BBR_RS14230 - HAMP domain-containing histidine kinase, at 967,216 to 968,289
_RS14230
BBR_RS14235: BBR_RS14235 - DNA-binding response regulator, at 968,289 to 969,011
_RS14235
BBR_RS14240: BBR_RS14240 - 1,4-alpha-glucan branching protein GlgB, at 969,042 to 971,294
_RS14240
Position (kb) 967
968
969 Strain fitness (log2 ratio) -2
-1
0
1
2 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.234 kb on - strand, within BBR_RS14220 at 966.281 kb on + strand, within BBR_RS14220 at 966.299 kb on - strand, within BBR_RS14220 at 966.367 kb on + strand at 966.367 kb on + strand at 966.386 kb on - strand at 966.391 kb on + strand at 966.409 kb on - strand at 966.509 kb on - strand at 966.528 kb on - strand at 966.530 kb on + strand at 966.546 kb on - strand at 966.546 kb on - strand at 966.551 kb on - strand at 966.551 kb on - strand at 966.627 kb on + strand, within BBR_RS14225 at 966.635 kb on - strand, within BBR_RS14225 at 966.635 kb on - strand, within BBR_RS14225 at 966.732 kb on - strand, within BBR_RS14225 at 966.830 kb on + strand, within BBR_RS14225 at 966.833 kb on - strand, within BBR_RS14225 at 966.840 kb on - strand, within BBR_RS14225 at 966.863 kb on - strand, within BBR_RS14225 at 966.863 kb on - strand, within BBR_RS14225 at 966.926 kb on - strand, within BBR_RS14225 at 967.025 kb on + strand, within BBR_RS14225 at 967.039 kb on - strand, within BBR_RS14225 at 967.053 kb on + strand, within BBR_RS14225 at 967.064 kb on + strand, within BBR_RS14225 at 967.074 kb on + strand, within BBR_RS14225 at 967.106 kb on + strand at 967.208 kb on + strand at 967.278 kb on + strand at 967.284 kb on + strand at 967.332 kb on + strand, within BBR_RS14230 at 967.340 kb on - strand, within BBR_RS14230 at 967.360 kb on + strand, within BBR_RS14230 at 967.365 kb on + strand, within BBR_RS14230 at 967.479 kb on - strand, within BBR_RS14230 at 967.503 kb on + strand, within BBR_RS14230 at 967.509 kb on - strand, within BBR_RS14230 at 967.541 kb on + strand, within BBR_RS14230 at 967.608 kb on - strand, within BBR_RS14230 at 967.609 kb on + strand, within BBR_RS14230 at 967.681 kb on - strand, within BBR_RS14230 at 967.698 kb on - strand, within BBR_RS14230 at 967.707 kb on - strand, within BBR_RS14230 at 967.715 kb on + strand, within BBR_RS14230 at 967.763 kb on - strand, within BBR_RS14230 at 967.763 kb on - strand, within BBR_RS14230 at 967.830 kb on - strand, within BBR_RS14230 at 967.863 kb on + strand, within BBR_RS14230 at 967.915 kb on - strand, within BBR_RS14230 at 967.919 kb on - strand, within BBR_RS14230 at 967.919 kb on - strand, within BBR_RS14230 at 967.920 kb on - strand, within BBR_RS14230 at 967.920 kb on - strand, within BBR_RS14230 at 968.005 kb on - strand, within BBR_RS14230 at 968.014 kb on - strand, within BBR_RS14230 at 968.043 kb on - strand, within BBR_RS14230 at 968.102 kb on - strand, within BBR_RS14230 at 968.117 kb on + strand, within BBR_RS14230 at 969.068 kb on - strand at 969.131 kb on + strand at 969.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 966,222 - BBR_RS14220 0.76 +1.2 966,222 - BBR_RS14220 0.76 -0.6 966,222 - BBR_RS14220 0.76 -0.4 966,234 - BBR_RS14220 0.78 -2.3 966,281 + BBR_RS14220 0.84 -1.0 966,299 - BBR_RS14220 0.86 -2.3 966,367 + -0.4 966,367 + +0.3 966,386 - -0.1 966,391 + +0.2 966,409 - -0.3 966,509 - +0.5 966,528 - +0.8 966,530 + +1.3 966,546 - +0.0 966,546 - -0.6 966,551 - +0.9 966,551 - -0.5 966,627 + BBR_RS14225 0.21 +0.9 966,635 - BBR_RS14225 0.22 -0.0 966,635 - BBR_RS14225 0.22 -0.0 966,732 - BBR_RS14225 0.37 -0.0 966,830 + BBR_RS14225 0.51 -0.5 966,833 - BBR_RS14225 0.52 -0.7 966,840 - BBR_RS14225 0.53 +0.2 966,863 - BBR_RS14225 0.56 -0.4 966,863 - BBR_RS14225 0.56 +0.7 966,926 - BBR_RS14225 0.65 -0.7 967,025 + BBR_RS14225 0.80 +0.5 967,039 - BBR_RS14225 0.82 -0.5 967,053 + BBR_RS14225 0.84 +0.6 967,064 + BBR_RS14225 0.86 +1.2 967,074 + BBR_RS14225 0.87 -0.6 967,106 + +0.4 967,208 + +0.2 967,278 + -1.0 967,284 + +0.7 967,332 + BBR_RS14230 0.11 -1.9 967,340 - BBR_RS14230 0.12 -1.8 967,360 + BBR_RS14230 0.13 -1.4 967,365 + BBR_RS14230 0.14 -0.4 967,479 - BBR_RS14230 0.24 -0.3 967,503 + BBR_RS14230 0.27 -2.5 967,509 - BBR_RS14230 0.27 -2.2 967,541 + BBR_RS14230 0.30 -1.5 967,608 - BBR_RS14230 0.36 -1.4 967,609 + BBR_RS14230 0.37 -0.3 967,681 - BBR_RS14230 0.43 -1.5 967,698 - BBR_RS14230 0.45 -0.3 967,707 - BBR_RS14230 0.46 +0.0 967,715 + BBR_RS14230 0.46 -0.1 967,763 - BBR_RS14230 0.51 +0.7 967,763 - BBR_RS14230 0.51 +2.3 967,830 - BBR_RS14230 0.57 +0.3 967,863 + BBR_RS14230 0.60 -1.9 967,915 - BBR_RS14230 0.65 -1.0 967,919 - BBR_RS14230 0.65 -0.3 967,919 - BBR_RS14230 0.65 -0.3 967,920 - BBR_RS14230 0.66 -2.0 967,920 - BBR_RS14230 0.66 -1.8 968,005 - BBR_RS14230 0.73 +0.0 968,014 - BBR_RS14230 0.74 -1.5 968,043 - BBR_RS14230 0.77 +2.3 968,102 - BBR_RS14230 0.82 +0.3 968,117 + BBR_RS14230 0.84 -1.8 969,068 - +1.2 969,131 + +2.3 969,208 + -0.3
Or see this region's nucleotide sequence