Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14190 and BBR_RS14200 are separated by 260 nucleotides BBR_RS14200 and BBR_RS14205 are separated by 45 nucleotides BBR_RS14205 and BBR_RS14210 overlap by 4 nucleotides
BBR_RS14190: BBR_RS14190 - transcriptional regulator, at 958,503 to 959,837
_RS14190
BBR_RS14200: BBR_RS14200 - WhiB family transcriptional regulator, at 960,098 to 960,397
_RS14200
BBR_RS14205: BBR_RS14205 - hypothetical protein, at 960,443 to 963,535
_RS14205
BBR_RS14210: BBR_RS14210 - hypothetical protein, at 963,532 to 965,073
_RS14210
Position (kb) 960
961
962
963
964 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 959.444 kb on + strand, within BBR_RS14190 at 959.444 kb on - strand, within BBR_RS14190 at 959.450 kb on + strand, within BBR_RS14190 at 959.452 kb on - strand, within BBR_RS14190 at 959.481 kb on - strand, within BBR_RS14190 at 959.491 kb on - strand, within BBR_RS14190 at 959.527 kb on - strand, within BBR_RS14190 at 959.575 kb on + strand, within BBR_RS14190 at 959.597 kb on + strand, within BBR_RS14190 at 959.597 kb on + strand, within BBR_RS14190 at 959.605 kb on - strand, within BBR_RS14190 at 959.612 kb on + strand, within BBR_RS14190 at 959.620 kb on - strand, within BBR_RS14190 at 959.637 kb on - strand, within BBR_RS14190 at 959.652 kb on - strand, within BBR_RS14190 at 959.688 kb on - strand, within BBR_RS14190 at 959.735 kb on - strand at 959.758 kb on - strand at 959.908 kb on - strand at 959.929 kb on - strand at 959.932 kb on + strand at 959.964 kb on - strand at 960.142 kb on + strand, within BBR_RS14200 at 960.142 kb on + strand, within BBR_RS14200 at 960.150 kb on - strand, within BBR_RS14200 at 960.154 kb on + strand, within BBR_RS14200 at 960.154 kb on + strand, within BBR_RS14200 at 960.162 kb on - strand, within BBR_RS14200 at 960.162 kb on - strand, within BBR_RS14200 at 960.195 kb on + strand, within BBR_RS14200 at 960.205 kb on + strand, within BBR_RS14200 at 960.342 kb on + strand, within BBR_RS14200 at 960.350 kb on - strand, within BBR_RS14200 at 960.379 kb on + strand at 960.397 kb on + strand at 960.439 kb on - strand at 960.516 kb on + strand at 960.946 kb on - strand, within BBR_RS14205 at 961.366 kb on + strand, within BBR_RS14205 at 961.838 kb on - strand, within BBR_RS14205 at 962.195 kb on - strand, within BBR_RS14205 at 962.742 kb on - strand, within BBR_RS14205 at 962.794 kb on + strand, within BBR_RS14205 at 962.802 kb on - strand, within BBR_RS14205 at 962.827 kb on + strand, within BBR_RS14205 at 962.830 kb on + strand, within BBR_RS14205 at 962.836 kb on + strand, within BBR_RS14205 at 962.946 kb on + strand, within BBR_RS14205 at 963.245 kb on + strand at 963.275 kb on - strand at 963.383 kb on + strand at 963.391 kb on + strand at 963.391 kb on + strand at 963.392 kb on + strand at 963.399 kb on - strand at 963.414 kb on + strand at 963.422 kb on - strand at 963.456 kb on - strand at 963.483 kb on - strand at 963.495 kb on + strand at 963.516 kb on + strand at 963.555 kb on + strand at 963.709 kb on - strand, within BBR_RS14210 at 963.993 kb on - strand, within BBR_RS14210 at 964.075 kb on + strand, within BBR_RS14210 at 964.448 kb on - strand, within BBR_RS14210 at 964.526 kb on - strand, within BBR_RS14210
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 959,444 + BBR_RS14190 0.70 -1.8 959,444 - BBR_RS14190 0.70 -1.5 959,450 + BBR_RS14190 0.71 -1.0 959,452 - BBR_RS14190 0.71 -1.3 959,481 - BBR_RS14190 0.73 -0.0 959,491 - BBR_RS14190 0.74 -1.4 959,527 - BBR_RS14190 0.77 -1.2 959,575 + BBR_RS14190 0.80 -0.1 959,597 + BBR_RS14190 0.82 -0.5 959,597 + BBR_RS14190 0.82 +0.1 959,605 - BBR_RS14190 0.83 -0.3 959,612 + BBR_RS14190 0.83 -0.8 959,620 - BBR_RS14190 0.84 -1.4 959,637 - BBR_RS14190 0.85 +0.1 959,652 - BBR_RS14190 0.86 -0.1 959,688 - BBR_RS14190 0.89 -0.3 959,735 - +0.0 959,758 - -1.1 959,908 - -0.6 959,929 - -0.3 959,932 + +0.0 959,964 - -0.5 960,142 + BBR_RS14200 0.15 +1.9 960,142 + BBR_RS14200 0.15 -0.9 960,150 - BBR_RS14200 0.17 +0.6 960,154 + BBR_RS14200 0.19 -1.3 960,154 + BBR_RS14200 0.19 -0.1 960,162 - BBR_RS14200 0.21 +0.8 960,162 - BBR_RS14200 0.21 -0.3 960,195 + BBR_RS14200 0.32 -0.7 960,205 + BBR_RS14200 0.36 -0.7 960,342 + BBR_RS14200 0.81 -2.0 960,350 - BBR_RS14200 0.84 +0.5 960,379 + -0.3 960,397 + +0.8 960,439 - -3.5 960,516 + +1.3 960,946 - BBR_RS14205 0.16 -0.3 961,366 + BBR_RS14205 0.30 +2.3 961,838 - BBR_RS14205 0.45 +1.3 962,195 - BBR_RS14205 0.57 -0.3 962,742 - BBR_RS14205 0.74 +0.7 962,794 + BBR_RS14205 0.76 -2.4 962,802 - BBR_RS14205 0.76 +1.3 962,827 + BBR_RS14205 0.77 -1.5 962,830 + BBR_RS14205 0.77 -2.4 962,836 + BBR_RS14205 0.77 -1.5 962,946 + BBR_RS14205 0.81 +0.7 963,245 + -1.8 963,275 - -2.2 963,383 + -1.2 963,391 + -0.5 963,391 + +0.0 963,392 + +0.1 963,399 - -0.6 963,414 + -0.4 963,422 - +0.0 963,456 - +0.5 963,483 - -0.4 963,495 + +0.2 963,516 + -2.6 963,555 + +0.3 963,709 - BBR_RS14210 0.11 -0.3 963,993 - BBR_RS14210 0.30 -1.5 964,075 + BBR_RS14210 0.35 +0.0 964,448 - BBR_RS14210 0.59 -0.3 964,526 - BBR_RS14210 0.64 -1.4
Or see this region's nucleotide sequence