Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14185 and BBR_RS14190 are separated by 203 nucleotides BBR_RS14190 and BBR_RS14200 are separated by 260 nucleotides BBR_RS14200 and BBR_RS14205 are separated by 45 nucleotides
BBR_RS14185: BBR_RS14185 - cell division protein FtsK, at 956,581 to 958,299
_RS14185
BBR_RS14190: BBR_RS14190 - transcriptional regulator, at 958,503 to 959,837
_RS14190
BBR_RS14200: BBR_RS14200 - WhiB family transcriptional regulator, at 960,098 to 960,397
_RS14200
BBR_RS14205: BBR_RS14205 - hypothetical protein, at 960,443 to 963,535
_RS14205
Position (kb)
958
959
960 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 957.504 kb on + strand, within BBR_RS14185 at 957.512 kb on - strand, within BBR_RS14185 at 957.602 kb on + strand, within BBR_RS14185 at 957.602 kb on + strand, within BBR_RS14185 at 957.656 kb on - strand, within BBR_RS14185 at 957.681 kb on - strand, within BBR_RS14185 at 957.692 kb on + strand, within BBR_RS14185 at 957.702 kb on + strand, within BBR_RS14185 at 957.723 kb on - strand, within BBR_RS14185 at 957.744 kb on - strand, within BBR_RS14185 at 957.746 kb on + strand, within BBR_RS14185 at 957.746 kb on + strand, within BBR_RS14185 at 957.754 kb on - strand, within BBR_RS14185 at 957.772 kb on - strand, within BBR_RS14185 at 957.824 kb on - strand, within BBR_RS14185 at 957.824 kb on - strand, within BBR_RS14185 at 957.829 kb on - strand, within BBR_RS14185 at 957.843 kb on - strand, within BBR_RS14185 at 957.924 kb on + strand, within BBR_RS14185 at 957.966 kb on - strand, within BBR_RS14185 at 957.981 kb on + strand, within BBR_RS14185 at 957.981 kb on + strand, within BBR_RS14185 at 957.981 kb on + strand, within BBR_RS14185 at 958.027 kb on + strand, within BBR_RS14185 at 958.074 kb on - strand, within BBR_RS14185 at 958.105 kb on - strand, within BBR_RS14185 at 958.118 kb on - strand, within BBR_RS14185 at 958.187 kb on - strand at 958.198 kb on + strand at 958.198 kb on + strand at 958.206 kb on - strand at 958.239 kb on + strand at 958.266 kb on + strand at 958.349 kb on - strand at 958.413 kb on + strand at 958.431 kb on + strand at 958.431 kb on + strand at 958.550 kb on - strand at 958.595 kb on + strand at 958.595 kb on + strand at 958.603 kb on - strand at 958.603 kb on - strand at 958.648 kb on - strand, within BBR_RS14190 at 958.751 kb on - strand, within BBR_RS14190 at 958.817 kb on - strand, within BBR_RS14190 at 958.817 kb on - strand, within BBR_RS14190 at 958.904 kb on - strand, within BBR_RS14190 at 958.944 kb on - strand, within BBR_RS14190 at 959.004 kb on + strand, within BBR_RS14190 at 959.012 kb on - strand, within BBR_RS14190 at 959.020 kb on - strand, within BBR_RS14190 at 959.024 kb on + strand, within BBR_RS14190 at 959.024 kb on - strand, within BBR_RS14190 at 959.029 kb on + strand, within BBR_RS14190 at 959.093 kb on - strand, within BBR_RS14190 at 959.119 kb on + strand, within BBR_RS14190 at 959.119 kb on + strand, within BBR_RS14190 at 959.119 kb on + strand, within BBR_RS14190 at 959.176 kb on - strand, within BBR_RS14190 at 959.176 kb on - strand, within BBR_RS14190 at 959.185 kb on - strand, within BBR_RS14190 at 959.194 kb on + strand, within BBR_RS14190 at 959.205 kb on + strand, within BBR_RS14190 at 959.205 kb on + strand, within BBR_RS14190 at 959.280 kb on - strand, within BBR_RS14190 at 959.280 kb on - strand, within BBR_RS14190 at 959.282 kb on + strand, within BBR_RS14190 at 959.358 kb on + strand, within BBR_RS14190 at 959.373 kb on - strand, within BBR_RS14190 at 959.394 kb on + strand, within BBR_RS14190 at 959.427 kb on + strand, within BBR_RS14190 at 959.444 kb on + strand, within BBR_RS14190 at 959.444 kb on - strand, within BBR_RS14190 at 959.450 kb on + strand, within BBR_RS14190 at 959.452 kb on - strand, within BBR_RS14190 at 959.481 kb on - strand, within BBR_RS14190 at 959.491 kb on - strand, within BBR_RS14190 at 959.527 kb on - strand, within BBR_RS14190 at 959.575 kb on + strand, within BBR_RS14190 at 959.597 kb on + strand, within BBR_RS14190 at 959.597 kb on + strand, within BBR_RS14190 at 959.605 kb on - strand, within BBR_RS14190 at 959.612 kb on + strand, within BBR_RS14190 at 959.620 kb on - strand, within BBR_RS14190 at 959.637 kb on - strand, within BBR_RS14190 at 959.652 kb on - strand, within BBR_RS14190 at 959.688 kb on - strand, within BBR_RS14190 at 959.735 kb on - strand at 959.758 kb on - strand at 959.908 kb on - strand at 959.929 kb on - strand at 959.932 kb on + strand at 959.964 kb on - strand at 960.142 kb on + strand, within BBR_RS14200 at 960.142 kb on + strand, within BBR_RS14200 at 960.150 kb on - strand, within BBR_RS14200 at 960.154 kb on + strand, within BBR_RS14200 at 960.154 kb on + strand, within BBR_RS14200 at 960.162 kb on - strand, within BBR_RS14200 at 960.162 kb on - strand, within BBR_RS14200 at 960.195 kb on + strand, within BBR_RS14200 at 960.205 kb on + strand, within BBR_RS14200 at 960.342 kb on + strand, within BBR_RS14200 at 960.350 kb on - strand, within BBR_RS14200 at 960.379 kb on + strand at 960.397 kb on + strand at 960.439 kb on - strand at 960.516 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 957,504 + BBR_RS14185 0.54 +0.1 957,512 - BBR_RS14185 0.54 -0.5 957,602 + BBR_RS14185 0.59 -0.1 957,602 + BBR_RS14185 0.59 +0.4 957,656 - BBR_RS14185 0.63 +0.5 957,681 - BBR_RS14185 0.64 +0.9 957,692 + BBR_RS14185 0.65 -0.7 957,702 + BBR_RS14185 0.65 +0.4 957,723 - BBR_RS14185 0.66 -0.3 957,744 - BBR_RS14185 0.68 +0.4 957,746 + BBR_RS14185 0.68 +0.1 957,746 + BBR_RS14185 0.68 -0.4 957,754 - BBR_RS14185 0.68 +0.1 957,772 - BBR_RS14185 0.69 +1.3 957,824 - BBR_RS14185 0.72 +0.0 957,824 - BBR_RS14185 0.72 -1.1 957,829 - BBR_RS14185 0.73 +0.0 957,843 - BBR_RS14185 0.73 -0.0 957,924 + BBR_RS14185 0.78 +0.8 957,966 - BBR_RS14185 0.81 +0.2 957,981 + BBR_RS14185 0.81 +0.2 957,981 + BBR_RS14185 0.81 -0.3 957,981 + BBR_RS14185 0.81 -0.8 958,027 + BBR_RS14185 0.84 +0.4 958,074 - BBR_RS14185 0.87 +0.0 958,105 - BBR_RS14185 0.89 -0.2 958,118 - BBR_RS14185 0.89 -0.0 958,187 - +1.0 958,198 + +0.2 958,198 + +0.7 958,206 - -0.7 958,239 + +0.2 958,266 + +0.9 958,349 - -0.0 958,413 + +0.1 958,431 + -0.4 958,431 + +0.4 958,550 - +0.3 958,595 + -2.0 958,595 + -1.2 958,603 - -0.4 958,603 - -2.1 958,648 - BBR_RS14190 0.11 -2.9 958,751 - BBR_RS14190 0.19 -1.8 958,817 - BBR_RS14190 0.24 -1.2 958,817 - BBR_RS14190 0.24 -2.4 958,904 - BBR_RS14190 0.30 -0.5 958,944 - BBR_RS14190 0.33 -1.2 959,004 + BBR_RS14190 0.38 -0.9 959,012 - BBR_RS14190 0.38 -0.6 959,020 - BBR_RS14190 0.39 -1.0 959,024 + BBR_RS14190 0.39 -1.9 959,024 - BBR_RS14190 0.39 -2.0 959,029 + BBR_RS14190 0.39 -0.9 959,093 - BBR_RS14190 0.44 -1.1 959,119 + BBR_RS14190 0.46 -0.7 959,119 + BBR_RS14190 0.46 -1.1 959,119 + BBR_RS14190 0.46 -0.3 959,176 - BBR_RS14190 0.50 -1.3 959,176 - BBR_RS14190 0.50 -0.9 959,185 - BBR_RS14190 0.51 -1.8 959,194 + BBR_RS14190 0.52 -1.6 959,205 + BBR_RS14190 0.53 -1.4 959,205 + BBR_RS14190 0.53 -3.4 959,280 - BBR_RS14190 0.58 -1.3 959,280 - BBR_RS14190 0.58 -0.7 959,282 + BBR_RS14190 0.58 -1.8 959,358 + BBR_RS14190 0.64 -1.0 959,373 - BBR_RS14190 0.65 -1.5 959,394 + BBR_RS14190 0.67 -2.0 959,427 + BBR_RS14190 0.69 -0.2 959,444 + BBR_RS14190 0.70 -1.8 959,444 - BBR_RS14190 0.70 -1.5 959,450 + BBR_RS14190 0.71 -1.0 959,452 - BBR_RS14190 0.71 -1.3 959,481 - BBR_RS14190 0.73 -0.0 959,491 - BBR_RS14190 0.74 -1.4 959,527 - BBR_RS14190 0.77 -1.2 959,575 + BBR_RS14190 0.80 -0.1 959,597 + BBR_RS14190 0.82 -0.5 959,597 + BBR_RS14190 0.82 +0.1 959,605 - BBR_RS14190 0.83 -0.3 959,612 + BBR_RS14190 0.83 -0.8 959,620 - BBR_RS14190 0.84 -1.4 959,637 - BBR_RS14190 0.85 +0.1 959,652 - BBR_RS14190 0.86 -0.1 959,688 - BBR_RS14190 0.89 -0.3 959,735 - +0.0 959,758 - -1.1 959,908 - -0.6 959,929 - -0.3 959,932 + +0.0 959,964 - -0.5 960,142 + BBR_RS14200 0.15 +1.9 960,142 + BBR_RS14200 0.15 -0.9 960,150 - BBR_RS14200 0.17 +0.6 960,154 + BBR_RS14200 0.19 -1.3 960,154 + BBR_RS14200 0.19 -0.1 960,162 - BBR_RS14200 0.21 +0.8 960,162 - BBR_RS14200 0.21 -0.3 960,195 + BBR_RS14200 0.32 -0.7 960,205 + BBR_RS14200 0.36 -0.7 960,342 + BBR_RS14200 0.81 -2.0 960,350 - BBR_RS14200 0.84 +0.5 960,379 + -0.3 960,397 + +0.8 960,439 - -3.5 960,516 + +1.3
Or see this region's nucleotide sequence