Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13885 and BBR_RS13890 overlap by 4 nucleotides BBR_RS13890 and BBR_RS13895 are separated by 228 nucleotides
BBR_RS13885: BBR_RS13885 - N-acetyl-gamma-glutamyl-phosphate reductase, at 894,905 to 895,999
_RS13885
BBR_RS13890: BBR_RS13890 - bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ, at 895,996 to 897,171
_RS13890
BBR_RS13895: BBR_RS13895 - acetylglutamate kinase, at 897,400 to 898,356
_RS13895
Position (kb)
895
896
897
898 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 894.999 kb on + strand at 895.087 kb on + strand, within BBR_RS13885 at 895.087 kb on + strand, within BBR_RS13885 at 895.092 kb on + strand, within BBR_RS13885 at 895.095 kb on - strand, within BBR_RS13885 at 895.095 kb on - strand, within BBR_RS13885 at 895.156 kb on + strand, within BBR_RS13885 at 895.193 kb on + strand, within BBR_RS13885 at 895.281 kb on + strand, within BBR_RS13885 at 895.283 kb on + strand, within BBR_RS13885 at 895.342 kb on + strand, within BBR_RS13885 at 895.342 kb on + strand, within BBR_RS13885 at 895.342 kb on + strand, within BBR_RS13885 at 895.344 kb on - strand, within BBR_RS13885 at 895.371 kb on + strand, within BBR_RS13885 at 895.383 kb on + strand, within BBR_RS13885 at 895.386 kb on + strand, within BBR_RS13885 at 895.415 kb on + strand, within BBR_RS13885 at 895.421 kb on + strand, within BBR_RS13885 at 895.429 kb on - strand, within BBR_RS13885 at 895.465 kb on - strand, within BBR_RS13885 at 895.468 kb on + strand, within BBR_RS13885 at 895.542 kb on - strand, within BBR_RS13885 at 895.542 kb on - strand, within BBR_RS13885 at 895.600 kb on + strand, within BBR_RS13885 at 895.627 kb on + strand, within BBR_RS13885 at 895.663 kb on + strand, within BBR_RS13885 at 895.663 kb on + strand, within BBR_RS13885 at 895.681 kb on - strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.708 kb on + strand, within BBR_RS13885 at 895.716 kb on - strand, within BBR_RS13885 at 895.716 kb on - strand, within BBR_RS13885 at 895.723 kb on + strand, within BBR_RS13885 at 895.763 kb on + strand, within BBR_RS13885 at 895.771 kb on - strand, within BBR_RS13885 at 895.771 kb on - strand, within BBR_RS13885 at 895.771 kb on - strand, within BBR_RS13885 at 895.794 kb on - strand, within BBR_RS13885 at 895.806 kb on + strand, within BBR_RS13885 at 895.848 kb on - strand, within BBR_RS13885 at 895.894 kb on + strand at 895.914 kb on - strand at 895.949 kb on - strand at 895.973 kb on - strand at 895.983 kb on - strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.986 kb on + strand at 895.988 kb on + strand at 895.988 kb on + strand at 895.989 kb on - strand at 895.989 kb on - strand at 896.062 kb on + strand at 896.094 kb on - strand at 896.112 kb on + strand at 896.141 kb on + strand, within BBR_RS13890 at 896.141 kb on + strand, within BBR_RS13890 at 896.144 kb on - strand, within BBR_RS13890 at 896.149 kb on + strand, within BBR_RS13890 at 896.149 kb on + strand, within BBR_RS13890 at 896.152 kb on + strand, within BBR_RS13890 at 896.163 kb on + strand, within BBR_RS13890 at 896.170 kb on + strand, within BBR_RS13890 at 896.223 kb on + strand, within BBR_RS13890 at 896.231 kb on + strand, within BBR_RS13890 at 896.236 kb on - strand, within BBR_RS13890 at 896.251 kb on - strand, within BBR_RS13890 at 896.265 kb on + strand, within BBR_RS13890 at 896.265 kb on + strand, within BBR_RS13890 at 896.304 kb on + strand, within BBR_RS13890 at 896.306 kb on + strand, within BBR_RS13890 at 896.321 kb on + strand, within BBR_RS13890 at 896.326 kb on + strand, within BBR_RS13890 at 896.409 kb on + strand, within BBR_RS13890 at 896.414 kb on + strand, within BBR_RS13890 at 896.425 kb on + strand, within BBR_RS13890 at 896.462 kb on + strand, within BBR_RS13890 at 896.462 kb on + strand, within BBR_RS13890 at 896.464 kb on + strand, within BBR_RS13890 at 896.472 kb on + strand, within BBR_RS13890 at 896.506 kb on + strand, within BBR_RS13890 at 896.513 kb on - strand, within BBR_RS13890 at 896.540 kb on + strand, within BBR_RS13890 at 896.550 kb on - strand, within BBR_RS13890 at 896.569 kb on + strand, within BBR_RS13890 at 896.574 kb on + strand, within BBR_RS13890 at 896.574 kb on + strand, within BBR_RS13890 at 896.579 kb on + strand, within BBR_RS13890 at 896.584 kb on + strand, within BBR_RS13890 at 896.589 kb on + strand, within BBR_RS13890 at 896.600 kb on + strand, within BBR_RS13890 at 896.604 kb on + strand, within BBR_RS13890 at 896.661 kb on + strand, within BBR_RS13890 at 896.720 kb on + strand, within BBR_RS13890 at 896.839 kb on + strand, within BBR_RS13890 at 896.845 kb on + strand, within BBR_RS13890 at 896.902 kb on + strand, within BBR_RS13890 at 896.920 kb on + strand, within BBR_RS13890 at 896.930 kb on + strand, within BBR_RS13890 at 896.976 kb on + strand, within BBR_RS13890 at 897.025 kb on + strand, within BBR_RS13890 at 897.033 kb on - strand, within BBR_RS13890 at 897.044 kb on - strand, within BBR_RS13890 at 897.047 kb on + strand, within BBR_RS13890 at 897.055 kb on - strand at 897.073 kb on + strand at 897.124 kb on + strand at 897.147 kb on + strand at 897.155 kb on - strand at 897.185 kb on + strand at 897.185 kb on + strand at 897.218 kb on - strand at 897.299 kb on + strand at 897.336 kb on - strand at 897.344 kb on + strand at 897.352 kb on + strand at 897.360 kb on - strand at 897.409 kb on + strand at 897.475 kb on - strand at 897.492 kb on + strand at 897.514 kb on + strand, within BBR_RS13895 at 897.585 kb on + strand, within BBR_RS13895 at 897.667 kb on + strand, within BBR_RS13895 at 897.668 kb on - strand, within BBR_RS13895 at 897.697 kb on + strand, within BBR_RS13895 at 897.742 kb on + strand, within BBR_RS13895 at 897.775 kb on + strand, within BBR_RS13895 at 897.834 kb on + strand, within BBR_RS13895 at 897.880 kb on + strand, within BBR_RS13895 at 897.918 kb on - strand, within BBR_RS13895 at 897.984 kb on + strand, within BBR_RS13895 at 898.000 kb on - strand, within BBR_RS13895 at 898.130 kb on + strand, within BBR_RS13895
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 894,999 + +0.2 895,087 + BBR_RS13885 0.17 +0.6 895,087 + BBR_RS13885 0.17 -0.3 895,092 + BBR_RS13885 0.17 +1.2 895,095 - BBR_RS13885 0.17 +0.1 895,095 - BBR_RS13885 0.17 -0.7 895,156 + BBR_RS13885 0.23 -0.3 895,193 + BBR_RS13885 0.26 -0.5 895,281 + BBR_RS13885 0.34 -0.1 895,283 + BBR_RS13885 0.35 +1.3 895,342 + BBR_RS13885 0.40 +0.7 895,342 + BBR_RS13885 0.40 +0.1 895,342 + BBR_RS13885 0.40 +0.7 895,344 - BBR_RS13885 0.40 +0.3 895,371 + BBR_RS13885 0.43 +0.0 895,383 + BBR_RS13885 0.44 -0.0 895,386 + BBR_RS13885 0.44 +0.2 895,415 + BBR_RS13885 0.47 -0.3 895,421 + BBR_RS13885 0.47 -0.4 895,429 - BBR_RS13885 0.48 +0.7 895,465 - BBR_RS13885 0.51 +1.1 895,468 + BBR_RS13885 0.51 +0.3 895,542 - BBR_RS13885 0.58 -2.1 895,542 - BBR_RS13885 0.58 -2.0 895,600 + BBR_RS13885 0.63 +0.5 895,627 + BBR_RS13885 0.66 +0.2 895,663 + BBR_RS13885 0.69 +0.4 895,663 + BBR_RS13885 0.69 +0.3 895,681 - BBR_RS13885 0.71 -1.0 895,708 + BBR_RS13885 0.73 +0.8 895,708 + BBR_RS13885 0.73 -0.0 895,708 + BBR_RS13885 0.73 +0.2 895,708 + BBR_RS13885 0.73 -0.3 895,716 - BBR_RS13885 0.74 -0.5 895,716 - BBR_RS13885 0.74 +0.1 895,723 + BBR_RS13885 0.75 -0.7 895,763 + BBR_RS13885 0.78 +1.3 895,771 - BBR_RS13885 0.79 -0.4 895,771 - BBR_RS13885 0.79 -0.6 895,771 - BBR_RS13885 0.79 +0.1 895,794 - BBR_RS13885 0.81 -1.9 895,806 + BBR_RS13885 0.82 +0.6 895,848 - BBR_RS13885 0.86 -0.0 895,894 + +1.9 895,914 - +0.9 895,949 - -0.6 895,973 - +0.3 895,983 - -1.0 895,986 + +0.2 895,986 + -0.8 895,986 + -0.2 895,986 + -0.2 895,986 + +0.2 895,986 + +0.7 895,988 + -0.7 895,988 + -0.3 895,989 - -0.3 895,989 - -0.8 896,062 + -0.7 896,094 - -0.8 896,112 + -1.4 896,141 + BBR_RS13890 0.12 -1.4 896,141 + BBR_RS13890 0.12 -1.3 896,144 - BBR_RS13890 0.13 -1.4 896,149 + BBR_RS13890 0.13 -1.3 896,149 + BBR_RS13890 0.13 -1.5 896,152 + BBR_RS13890 0.13 -1.4 896,163 + BBR_RS13890 0.14 -1.6 896,170 + BBR_RS13890 0.15 -0.9 896,223 + BBR_RS13890 0.19 -0.6 896,231 + BBR_RS13890 0.20 -1.2 896,236 - BBR_RS13890 0.20 +0.7 896,251 - BBR_RS13890 0.22 +0.7 896,265 + BBR_RS13890 0.23 -1.7 896,265 + BBR_RS13890 0.23 -1.8 896,304 + BBR_RS13890 0.26 -0.7 896,306 + BBR_RS13890 0.26 -2.0 896,321 + BBR_RS13890 0.28 -2.1 896,326 + BBR_RS13890 0.28 -1.8 896,409 + BBR_RS13890 0.35 -0.6 896,414 + BBR_RS13890 0.36 -1.6 896,425 + BBR_RS13890 0.36 -1.5 896,462 + BBR_RS13890 0.40 -1.9 896,462 + BBR_RS13890 0.40 -1.3 896,464 + BBR_RS13890 0.40 +0.4 896,472 + BBR_RS13890 0.40 -0.4 896,506 + BBR_RS13890 0.43 -1.5 896,513 - BBR_RS13890 0.44 -1.1 896,540 + BBR_RS13890 0.46 -2.3 896,550 - BBR_RS13890 0.47 +0.5 896,569 + BBR_RS13890 0.49 -1.0 896,574 + BBR_RS13890 0.49 -2.2 896,574 + BBR_RS13890 0.49 -1.6 896,579 + BBR_RS13890 0.50 -0.8 896,584 + BBR_RS13890 0.50 -2.4 896,589 + BBR_RS13890 0.50 -0.6 896,600 + BBR_RS13890 0.51 -1.4 896,604 + BBR_RS13890 0.52 -1.8 896,661 + BBR_RS13890 0.57 -0.3 896,720 + BBR_RS13890 0.62 -1.1 896,839 + BBR_RS13890 0.72 -2.0 896,845 + BBR_RS13890 0.72 -1.0 896,902 + BBR_RS13890 0.77 -2.0 896,920 + BBR_RS13890 0.79 -1.9 896,930 + BBR_RS13890 0.79 -0.9 896,976 + BBR_RS13890 0.83 -1.1 897,025 + BBR_RS13890 0.88 -0.2 897,033 - BBR_RS13890 0.88 -0.6 897,044 - BBR_RS13890 0.89 -2.1 897,047 + BBR_RS13890 0.89 -0.6 897,055 - -1.1 897,073 + -1.8 897,124 + -0.8 897,147 + -1.4 897,155 - -0.2 897,185 + -0.3 897,185 + -0.8 897,218 - -0.0 897,299 + -0.5 897,336 - -0.0 897,344 + +0.1 897,352 + +0.6 897,360 - -0.4 897,409 + +0.1 897,475 - -0.2 897,492 + -1.4 897,514 + BBR_RS13895 0.12 -0.4 897,585 + BBR_RS13895 0.19 -0.3 897,667 + BBR_RS13895 0.28 +0.7 897,668 - BBR_RS13895 0.28 +0.7 897,697 + BBR_RS13895 0.31 -0.1 897,742 + BBR_RS13895 0.36 +1.1 897,775 + BBR_RS13895 0.39 +0.4 897,834 + BBR_RS13895 0.45 +0.0 897,880 + BBR_RS13895 0.50 -0.2 897,918 - BBR_RS13895 0.54 +0.6 897,984 + BBR_RS13895 0.61 +0.7 898,000 - BBR_RS13895 0.63 +1.3 898,130 + BBR_RS13895 0.76 +0.4
Or see this region's nucleotide sequence