Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13710 and BBR_RS13715 are separated by 56 nucleotides BBR_RS13715 and BBR_RS13720 are separated by 105 nucleotides BBR_RS13720 and BBR_RS13725 are separated by 82 nucleotides
BBR_RS13710: BBR_RS13710 - PHP domain-containing protein, at 848,925 to 849,818
_RS13710
BBR_RS13715: BBR_RS13715 - DUF3107 domain-containing protein, at 849,875 to 850,099
_RS13715
BBR_RS13720: BBR_RS13720 - hypothetical protein, at 850,205 to 851,836
_RS13720
BBR_RS13725: BBR_RS13725 - ATP-dependent helicase, at 851,919 to 853,466
_RS13725
Position (kb) 850
851
852 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 849.271 kb on - strand, within BBR_RS13710 at 849.305 kb on + strand, within BBR_RS13710 at 849.305 kb on + strand, within BBR_RS13710 at 849.338 kb on + strand, within BBR_RS13710 at 849.338 kb on + strand, within BBR_RS13710 at 849.346 kb on - strand, within BBR_RS13710 at 849.346 kb on - strand, within BBR_RS13710 at 849.346 kb on - strand, within BBR_RS13710 at 849.358 kb on + strand, within BBR_RS13710 at 849.358 kb on + strand, within BBR_RS13710 at 849.367 kb on - strand, within BBR_RS13710 at 849.369 kb on - strand, within BBR_RS13710 at 849.369 kb on - strand, within BBR_RS13710 at 849.370 kb on - strand, within BBR_RS13710 at 849.476 kb on + strand, within BBR_RS13710 at 849.476 kb on + strand, within BBR_RS13710 at 849.531 kb on - strand, within BBR_RS13710 at 849.549 kb on + strand, within BBR_RS13710 at 849.617 kb on - strand, within BBR_RS13710 at 849.619 kb on + strand, within BBR_RS13710 at 849.627 kb on + strand, within BBR_RS13710 at 849.646 kb on + strand, within BBR_RS13710 at 849.654 kb on - strand, within BBR_RS13710 at 849.664 kb on - strand, within BBR_RS13710 at 849.682 kb on + strand, within BBR_RS13710 at 849.717 kb on - strand, within BBR_RS13710 at 849.718 kb on + strand, within BBR_RS13710 at 849.726 kb on - strand, within BBR_RS13710 at 849.812 kb on - strand at 849.818 kb on + strand at 849.819 kb on + strand at 849.848 kb on + strand at 849.856 kb on - strand at 849.863 kb on - strand at 849.872 kb on + strand at 849.872 kb on + strand at 849.874 kb on + strand at 849.882 kb on - strand at 849.929 kb on + strand, within BBR_RS13715 at 849.937 kb on - strand, within BBR_RS13715 at 849.944 kb on - strand, within BBR_RS13715 at 849.999 kb on + strand, within BBR_RS13715 at 850.006 kb on - strand, within BBR_RS13715 at 850.055 kb on - strand, within BBR_RS13715 at 850.105 kb on - strand at 850.115 kb on - strand at 850.117 kb on - strand at 850.174 kb on - strand at 850.253 kb on - strand at 850.258 kb on - strand at 850.277 kb on - strand at 850.277 kb on - strand at 850.277 kb on - strand at 850.300 kb on - strand at 850.300 kb on - strand at 850.316 kb on - strand at 850.362 kb on + strand at 850.401 kb on + strand, within BBR_RS13720 at 850.401 kb on + strand, within BBR_RS13720 at 850.401 kb on + strand, within BBR_RS13720 at 850.409 kb on - strand, within BBR_RS13720 at 850.409 kb on - strand, within BBR_RS13720 at 850.495 kb on - strand, within BBR_RS13720 at 850.540 kb on + strand, within BBR_RS13720 at 850.545 kb on + strand, within BBR_RS13720 at 850.553 kb on - strand, within BBR_RS13720 at 850.553 kb on - strand, within BBR_RS13720 at 850.602 kb on + strand, within BBR_RS13720 at 850.602 kb on + strand, within BBR_RS13720 at 850.604 kb on - strand, within BBR_RS13720 at 850.604 kb on - strand, within BBR_RS13720 at 850.681 kb on + strand, within BBR_RS13720 at 850.689 kb on - strand, within BBR_RS13720 at 850.689 kb on - strand, within BBR_RS13720 at 850.701 kb on - strand, within BBR_RS13720 at 850.701 kb on - strand, within BBR_RS13720 at 850.724 kb on - strand, within BBR_RS13720 at 850.806 kb on + strand, within BBR_RS13720 at 850.806 kb on + strand, within BBR_RS13720 at 850.851 kb on + strand, within BBR_RS13720 at 850.945 kb on + strand, within BBR_RS13720 at 851.839 kb on + strand at 851.844 kb on - strand at 851.847 kb on - strand at 851.855 kb on - strand at 851.866 kb on - strand at 851.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 849,271 - BBR_RS13710 0.39 -0.5 849,305 + BBR_RS13710 0.43 +0.2 849,305 + BBR_RS13710 0.43 +0.3 849,338 + BBR_RS13710 0.46 +0.1 849,338 + BBR_RS13710 0.46 -0.2 849,346 - BBR_RS13710 0.47 +0.6 849,346 - BBR_RS13710 0.47 -0.5 849,346 - BBR_RS13710 0.47 +0.2 849,358 + BBR_RS13710 0.48 +0.7 849,358 + BBR_RS13710 0.48 +0.0 849,367 - BBR_RS13710 0.49 +0.3 849,369 - BBR_RS13710 0.50 +0.2 849,369 - BBR_RS13710 0.50 +0.3 849,370 - BBR_RS13710 0.50 -0.2 849,476 + BBR_RS13710 0.62 -0.3 849,476 + BBR_RS13710 0.62 +0.5 849,531 - BBR_RS13710 0.68 +0.4 849,549 + BBR_RS13710 0.70 -0.4 849,617 - BBR_RS13710 0.77 -0.5 849,619 + BBR_RS13710 0.78 +0.2 849,627 + BBR_RS13710 0.79 -0.1 849,646 + BBR_RS13710 0.81 -1.1 849,654 - BBR_RS13710 0.82 +0.9 849,664 - BBR_RS13710 0.83 +0.1 849,682 + BBR_RS13710 0.85 +0.5 849,717 - BBR_RS13710 0.89 +0.6 849,718 + BBR_RS13710 0.89 +0.0 849,726 - BBR_RS13710 0.90 +0.2 849,812 - +0.3 849,818 + -0.0 849,819 + +0.3 849,848 + +0.6 849,856 - -0.1 849,863 - -0.5 849,872 + -0.2 849,872 + -0.4 849,874 + +1.4 849,882 - +0.8 849,929 + BBR_RS13715 0.24 -0.5 849,937 - BBR_RS13715 0.28 -0.1 849,944 - BBR_RS13715 0.31 +0.1 849,999 + BBR_RS13715 0.55 -0.1 850,006 - BBR_RS13715 0.58 +0.4 850,055 - BBR_RS13715 0.80 +0.6 850,105 - -0.8 850,115 - -1.0 850,117 - -1.0 850,174 - -0.4 850,253 - -0.8 850,258 - +0.2 850,277 - -1.0 850,277 - -0.5 850,277 - +0.1 850,300 - -0.0 850,300 - -0.4 850,316 - -1.2 850,362 + +0.2 850,401 + BBR_RS13720 0.12 -0.3 850,401 + BBR_RS13720 0.12 -0.3 850,401 + BBR_RS13720 0.12 -1.9 850,409 - BBR_RS13720 0.12 -1.7 850,409 - BBR_RS13720 0.12 -0.0 850,495 - BBR_RS13720 0.18 +0.2 850,540 + BBR_RS13720 0.21 -0.9 850,545 + BBR_RS13720 0.21 +1.9 850,553 - BBR_RS13720 0.21 -0.5 850,553 - BBR_RS13720 0.21 -0.6 850,602 + BBR_RS13720 0.24 -2.4 850,602 + BBR_RS13720 0.24 -0.3 850,604 - BBR_RS13720 0.24 -0.2 850,604 - BBR_RS13720 0.24 -1.6 850,681 + BBR_RS13720 0.29 -1.4 850,689 - BBR_RS13720 0.30 -1.0 850,689 - BBR_RS13720 0.30 -0.4 850,701 - BBR_RS13720 0.30 -0.6 850,701 - BBR_RS13720 0.30 -0.8 850,724 - BBR_RS13720 0.32 -3.1 850,806 + BBR_RS13720 0.37 -0.7 850,806 + BBR_RS13720 0.37 -2.5 850,851 + BBR_RS13720 0.40 -0.2 850,945 + BBR_RS13720 0.45 +0.3 851,839 + +2.0 851,844 - -0.4 851,847 - -0.1 851,855 - -0.2 851,866 - -0.2 851,874 - +0.0
Or see this region's nucleotide sequence