Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13290 and BBR_RS13295 are separated by 66 nucleotides BBR_RS13295 and BBR_RS13300 overlap by 17 nucleotides BBR_RS13300 and BBR_RS13305 are separated by 8 nucleotides BBR_RS13305 and BBR_RS13310 are separated by 46 nucleotides
BBR_RS13290: BBR_RS13290 - Zn-dependent oxidoreductase, at 757,551 to 758,543
_RS13290
BBR_RS13295: BBR_RS13295 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 758,610 to 759,110
_RS13295
BBR_RS13300: BBR_RS13300 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 759,094 to 760,272
_RS13300
BBR_RS13305: BBR_RS13305 - transcriptional repressor, at 760,281 to 760,709
_RS13305
BBR_RS13310: BBR_RS13310 - hypothetical protein, at 760,756 to 761,466
_RS13310
Position (kb)
759
760
761 Strain fitness (log2 ratio)
-2
-1
0
1 at 758.113 kb on + strand, within BBR_RS13290 at 758.113 kb on + strand, within BBR_RS13290 at 758.113 kb on + strand, within BBR_RS13290 at 758.113 kb on + strand, within BBR_RS13290 at 758.113 kb on + strand, within BBR_RS13290 at 758.121 kb on - strand, within BBR_RS13290 at 758.121 kb on - strand, within BBR_RS13290 at 758.121 kb on - strand, within BBR_RS13290 at 758.121 kb on - strand, within BBR_RS13290 at 758.123 kb on - strand, within BBR_RS13290 at 758.123 kb on - strand, within BBR_RS13290 at 758.125 kb on + strand, within BBR_RS13290 at 758.139 kb on - strand, within BBR_RS13290 at 758.160 kb on + strand, within BBR_RS13290 at 758.161 kb on - strand, within BBR_RS13290 at 758.168 kb on - strand, within BBR_RS13290 at 758.224 kb on - strand, within BBR_RS13290 at 758.236 kb on - strand, within BBR_RS13290 at 758.329 kb on + strand, within BBR_RS13290 at 758.336 kb on + strand, within BBR_RS13290 at 758.337 kb on - strand, within BBR_RS13290 at 758.351 kb on + strand, within BBR_RS13290 at 758.372 kb on + strand, within BBR_RS13290 at 758.372 kb on + strand, within BBR_RS13290 at 758.380 kb on - strand, within BBR_RS13290 at 758.380 kb on - strand, within BBR_RS13290 at 758.457 kb on + strand at 758.545 kb on - strand at 758.559 kb on + strand at 758.566 kb on + strand at 758.566 kb on + strand at 758.574 kb on - strand at 758.574 kb on - strand at 759.413 kb on + strand, within BBR_RS13300 at 759.498 kb on - strand, within BBR_RS13300 at 759.595 kb on - strand, within BBR_RS13300 at 760.274 kb on - strand at 760.281 kb on - strand at 760.337 kb on + strand, within BBR_RS13305 at 760.342 kb on + strand, within BBR_RS13305 at 760.350 kb on - strand, within BBR_RS13305 at 760.350 kb on - strand, within BBR_RS13305 at 760.384 kb on - strand, within BBR_RS13305 at 760.518 kb on + strand, within BBR_RS13305 at 760.602 kb on + strand, within BBR_RS13305 at 760.607 kb on + strand, within BBR_RS13305 at 760.630 kb on + strand, within BBR_RS13305 at 760.630 kb on + strand, within BBR_RS13305 at 760.632 kb on + strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.685 kb on - strand at 760.689 kb on + strand at 760.712 kb on - strand at 760.755 kb on + strand at 760.758 kb on + strand at 760.758 kb on + strand at 760.793 kb on + strand at 760.793 kb on + strand at 760.793 kb on + strand at 760.793 kb on + strand at 760.818 kb on + strand at 760.836 kb on + strand, within BBR_RS13310 at 760.907 kb on + strand, within BBR_RS13310 at 760.937 kb on + strand, within BBR_RS13310 at 760.945 kb on - strand, within BBR_RS13310 at 761.001 kb on - strand, within BBR_RS13310 at 761.006 kb on - strand, within BBR_RS13310 at 761.031 kb on + strand, within BBR_RS13310 at 761.046 kb on + strand, within BBR_RS13310 at 761.054 kb on - strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.090 kb on - strand, within BBR_RS13310 at 761.090 kb on - strand, within BBR_RS13310 at 761.109 kb on - strand, within BBR_RS13310 at 761.188 kb on + strand, within BBR_RS13310 at 761.196 kb on + strand, within BBR_RS13310 at 761.198 kb on - strand, within BBR_RS13310 at 761.198 kb on - strand, within BBR_RS13310 at 761.206 kb on + strand, within BBR_RS13310 at 761.207 kb on - strand, within BBR_RS13310
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 758,113 + BBR_RS13290 0.57 +0.6 758,113 + BBR_RS13290 0.57 +0.8 758,113 + BBR_RS13290 0.57 +0.7 758,113 + BBR_RS13290 0.57 +0.0 758,113 + BBR_RS13290 0.57 -0.1 758,121 - BBR_RS13290 0.57 -0.4 758,121 - BBR_RS13290 0.57 -0.6 758,121 - BBR_RS13290 0.57 -0.3 758,121 - BBR_RS13290 0.57 +0.3 758,123 - BBR_RS13290 0.58 +1.3 758,123 - BBR_RS13290 0.58 +0.5 758,125 + BBR_RS13290 0.58 +0.5 758,139 - BBR_RS13290 0.59 -0.0 758,160 + BBR_RS13290 0.61 +0.0 758,161 - BBR_RS13290 0.61 -0.1 758,168 - BBR_RS13290 0.62 +0.4 758,224 - BBR_RS13290 0.68 -0.4 758,236 - BBR_RS13290 0.69 +0.5 758,329 + BBR_RS13290 0.78 +0.0 758,336 + BBR_RS13290 0.79 -0.6 758,337 - BBR_RS13290 0.79 -1.8 758,351 + BBR_RS13290 0.81 -1.9 758,372 + BBR_RS13290 0.83 +0.2 758,372 + BBR_RS13290 0.83 +0.2 758,380 - BBR_RS13290 0.83 -0.6 758,380 - BBR_RS13290 0.83 -0.4 758,457 + -0.3 758,545 - -1.1 758,559 + +0.4 758,566 + -0.1 758,566 + +0.2 758,574 - -0.1 758,574 - +0.9 759,413 + BBR_RS13300 0.27 +0.0 759,498 - BBR_RS13300 0.34 +0.3 759,595 - BBR_RS13300 0.42 -0.3 760,274 - +0.1 760,281 - -1.0 760,337 + BBR_RS13305 0.13 -0.8 760,342 + BBR_RS13305 0.14 +0.2 760,350 - BBR_RS13305 0.16 -0.2 760,350 - BBR_RS13305 0.16 +0.2 760,384 - BBR_RS13305 0.24 -1.3 760,518 + BBR_RS13305 0.55 -0.5 760,602 + BBR_RS13305 0.75 -0.9 760,607 + BBR_RS13305 0.76 -0.5 760,630 + BBR_RS13305 0.81 -0.4 760,630 + BBR_RS13305 0.81 -1.1 760,632 + BBR_RS13305 0.82 -0.2 760,638 - BBR_RS13305 0.83 +0.5 760,638 - BBR_RS13305 0.83 -2.1 760,638 - BBR_RS13305 0.83 +0.6 760,638 - BBR_RS13305 0.83 -1.4 760,685 - -0.9 760,689 + +0.4 760,712 - -0.0 760,755 + +0.6 760,758 + -0.9 760,758 + +0.3 760,793 + +0.3 760,793 + +0.7 760,793 + -0.0 760,793 + +0.7 760,818 + +0.4 760,836 + BBR_RS13310 0.11 +0.1 760,907 + BBR_RS13310 0.21 +0.3 760,937 + BBR_RS13310 0.25 -0.0 760,945 - BBR_RS13310 0.27 -0.3 761,001 - BBR_RS13310 0.34 -0.9 761,006 - BBR_RS13310 0.35 -0.2 761,031 + BBR_RS13310 0.39 -0.1 761,046 + BBR_RS13310 0.41 +0.1 761,054 - BBR_RS13310 0.42 -0.4 761,082 + BBR_RS13310 0.46 +0.0 761,082 + BBR_RS13310 0.46 +0.5 761,082 + BBR_RS13310 0.46 +0.5 761,082 + BBR_RS13310 0.46 +0.3 761,090 - BBR_RS13310 0.47 +0.1 761,090 - BBR_RS13310 0.47 -0.1 761,109 - BBR_RS13310 0.50 +0.4 761,188 + BBR_RS13310 0.61 -0.1 761,196 + BBR_RS13310 0.62 +0.9 761,198 - BBR_RS13310 0.62 -0.1 761,198 - BBR_RS13310 0.62 -0.1 761,206 + BBR_RS13310 0.63 +0.1 761,207 - BBR_RS13310 0.63 +0.4
Or see this region's nucleotide sequence