Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13035 and BBR_RS13040 are separated by 78 nucleotides BBR_RS13040 and BBR_RS13045 are separated by 113 nucleotides BBR_RS13045 and BBR_RS13050 are separated by 121 nucleotides
BBR_RS13035: BBR_RS13035 - ABC transporter permease, at 697,844 to 698,767
_RS13035
BBR_RS13040: BBR_RS13040 - CHAP domain-containing protein, at 698,846 to 700,192
_RS13040
BBR_RS13045: BBR_RS13045 - SsrA-binding protein, at 700,306 to 700,782
_RS13045
BBR_RS13050: BBR_RS13050 - ABC transporter substrate-binding protein, at 700,904 to 701,857
_RS13050
Position (kb) 698
699
700
701 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 697.895 kb on - strand at 697.906 kb on + strand at 697.911 kb on + strand at 698.029 kb on - strand, within BBR_RS13035 at 698.048 kb on - strand, within BBR_RS13035 at 698.221 kb on - strand, within BBR_RS13035 at 698.318 kb on - strand, within BBR_RS13035 at 698.420 kb on - strand, within BBR_RS13035 at 698.779 kb on - strand at 698.781 kb on + strand at 698.810 kb on + strand at 698.876 kb on - strand at 699.154 kb on + strand, within BBR_RS13040 at 699.290 kb on + strand, within BBR_RS13040 at 699.429 kb on - strand, within BBR_RS13040 at 699.481 kb on + strand, within BBR_RS13040 at 699.671 kb on + strand, within BBR_RS13040 at 699.773 kb on - strand, within BBR_RS13040 at 699.833 kb on + strand, within BBR_RS13040 at 699.836 kb on - strand, within BBR_RS13040 at 699.868 kb on + strand, within BBR_RS13040 at 700.022 kb on - strand, within BBR_RS13040 at 700.191 kb on + strand at 700.281 kb on - strand at 700.281 kb on - strand at 700.288 kb on + strand at 700.739 kb on - strand at 700.813 kb on + strand at 700.819 kb on + strand at 700.837 kb on + strand at 700.853 kb on - strand at 700.943 kb on + strand at 700.951 kb on - strand at 700.951 kb on - strand at 700.951 kb on - strand at 700.961 kb on - strand at 700.961 kb on - strand at 700.994 kb on + strand at 700.995 kb on - strand at 701.016 kb on - strand, within BBR_RS13050 at 701.030 kb on + strand, within BBR_RS13050 at 701.137 kb on - strand, within BBR_RS13050 at 701.163 kb on + strand, within BBR_RS13050
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 697,895 - +0.7 697,906 + -1.2 697,911 + +1.3 698,029 - BBR_RS13035 0.20 -0.7 698,048 - BBR_RS13035 0.22 +2.3 698,221 - BBR_RS13035 0.41 +0.7 698,318 - BBR_RS13035 0.51 +0.7 698,420 - BBR_RS13035 0.62 -1.1 698,779 - -0.3 698,781 + +1.6 698,810 + +0.1 698,876 - +2.3 699,154 + BBR_RS13040 0.23 +1.3 699,290 + BBR_RS13040 0.33 -3.2 699,429 - BBR_RS13040 0.43 -1.3 699,481 + BBR_RS13040 0.47 -1.1 699,671 + BBR_RS13040 0.61 +0.0 699,773 - BBR_RS13040 0.69 +2.3 699,833 + BBR_RS13040 0.73 -1.0 699,836 - BBR_RS13040 0.73 -3.9 699,868 + BBR_RS13040 0.76 +2.3 700,022 - BBR_RS13040 0.87 +2.3 700,191 + +0.6 700,281 - +0.3 700,281 - +0.7 700,288 + +0.7 700,739 - -0.4 700,813 + +0.5 700,819 + +0.2 700,837 + +0.3 700,853 - -0.0 700,943 + +0.5 700,951 - +0.6 700,951 - -0.7 700,951 - -0.2 700,961 - -0.6 700,961 - +0.5 700,994 + -0.1 700,995 - +0.3 701,016 - BBR_RS13050 0.12 -1.0 701,030 + BBR_RS13050 0.13 +0.2 701,137 - BBR_RS13050 0.24 +0.6 701,163 + BBR_RS13050 0.27 -0.9
Or see this region's nucleotide sequence