Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13030 and BBR_RS13035 are separated by 3 nucleotides BBR_RS13035 and BBR_RS13040 are separated by 78 nucleotides
BBR_RS13030: BBR_RS13030 - cell division ATP-binding protein FtsE, at 696,644 to 697,840
_RS13030
BBR_RS13035: BBR_RS13035 - ABC transporter permease, at 697,844 to 698,767
_RS13035
BBR_RS13040: BBR_RS13040 - CHAP domain-containing protein, at 698,846 to 700,192
_RS13040
Position (kb) 697
698
699 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 696.853 kb on - strand, within BBR_RS13030 at 696.885 kb on + strand, within BBR_RS13030 at 697.152 kb on + strand, within BBR_RS13030 at 697.165 kb on - strand, within BBR_RS13030 at 697.436 kb on + strand, within BBR_RS13030 at 697.539 kb on - strand, within BBR_RS13030 at 697.610 kb on + strand, within BBR_RS13030 at 697.618 kb on + strand, within BBR_RS13030 at 697.652 kb on + strand, within BBR_RS13030 at 697.746 kb on + strand at 697.746 kb on + strand at 697.777 kb on + strand at 697.782 kb on + strand at 697.782 kb on + strand at 697.787 kb on + strand at 697.802 kb on + strand at 697.833 kb on - strand at 697.835 kb on + strand at 697.840 kb on + strand at 697.895 kb on - strand at 697.906 kb on + strand at 697.911 kb on + strand at 698.029 kb on - strand, within BBR_RS13035 at 698.048 kb on - strand, within BBR_RS13035 at 698.221 kb on - strand, within BBR_RS13035 at 698.318 kb on - strand, within BBR_RS13035 at 698.420 kb on - strand, within BBR_RS13035 at 698.779 kb on - strand at 698.781 kb on + strand at 698.810 kb on + strand at 698.876 kb on - strand at 699.154 kb on + strand, within BBR_RS13040 at 699.290 kb on + strand, within BBR_RS13040 at 699.429 kb on - strand, within BBR_RS13040 at 699.481 kb on + strand, within BBR_RS13040 at 699.671 kb on + strand, within BBR_RS13040
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 696,853 - BBR_RS13030 0.17 +2.3 696,885 + BBR_RS13030 0.20 +2.3 697,152 + BBR_RS13030 0.42 +2.3 697,165 - BBR_RS13030 0.44 +0.7 697,436 + BBR_RS13030 0.66 +1.3 697,539 - BBR_RS13030 0.75 -1.1 697,610 + BBR_RS13030 0.81 -0.7 697,618 + BBR_RS13030 0.81 +0.3 697,652 + BBR_RS13030 0.84 -2.2 697,746 + -0.5 697,746 + -0.4 697,777 + -0.6 697,782 + -0.4 697,782 + -1.0 697,787 + +0.2 697,802 + -0.5 697,833 - -0.2 697,835 + -0.8 697,840 + -0.8 697,895 - +0.7 697,906 + -1.2 697,911 + +1.3 698,029 - BBR_RS13035 0.20 -0.7 698,048 - BBR_RS13035 0.22 +2.3 698,221 - BBR_RS13035 0.41 +0.7 698,318 - BBR_RS13035 0.51 +0.7 698,420 - BBR_RS13035 0.62 -1.1 698,779 - -0.3 698,781 + +1.6 698,810 + +0.1 698,876 - +2.3 699,154 + BBR_RS13040 0.23 +1.3 699,290 + BBR_RS13040 0.33 -3.2 699,429 - BBR_RS13040 0.43 -1.3 699,481 + BBR_RS13040 0.47 -1.1 699,671 + BBR_RS13040 0.61 +0.0
Or see this region's nucleotide sequence