Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12980 and BBR_RS12985 are separated by 171 nucleotides BBR_RS12985 and BBR_RS12990 are separated by 87 nucleotides BBR_RS12990 and BBR_RS12995 are separated by 105 nucleotides
BBR_RS12980: BBR_RS12980 - ATP-dependent DNA helicase UvrD/REP family, at 679,178 to 683,113
_RS12980
BBR_RS12985: BBR_RS12985 - MFS transporter, at 683,285 to 684,604
_RS12985
BBR_RS12990: BBR_RS12990 - 4-hydroxy-tetrahydrodipicolinate reductase, at 684,692 to 685,447
_RS12990
BBR_RS12995: BBR_RS12995 - 4-hydroxy-tetrahydrodipicolinate synthase, at 685,553 to 686,458
_RS12995
Position (kb) 683
684
685 Strain fitness (log2 ratio) -2
-1
0
1
2 at 682.294 kb on - strand, within BBR_RS12980 at 682.310 kb on + strand, within BBR_RS12980 at 682.328 kb on - strand, within BBR_RS12980 at 682.332 kb on - strand, within BBR_RS12980 at 682.342 kb on + strand, within BBR_RS12980 at 682.342 kb on + strand, within BBR_RS12980 at 682.345 kb on - strand, within BBR_RS12980 at 682.347 kb on + strand, within BBR_RS12980 at 682.365 kb on - strand, within BBR_RS12980 at 682.378 kb on + strand, within BBR_RS12980 at 682.396 kb on - strand, within BBR_RS12980 at 682.412 kb on - strand, within BBR_RS12980 at 682.442 kb on - strand, within BBR_RS12980 at 682.469 kb on - strand, within BBR_RS12980 at 682.470 kb on + strand, within BBR_RS12980 at 682.553 kb on + strand, within BBR_RS12980 at 682.600 kb on - strand, within BBR_RS12980 at 682.608 kb on - strand, within BBR_RS12980 at 682.624 kb on - strand, within BBR_RS12980 at 682.661 kb on + strand, within BBR_RS12980 at 682.674 kb on - strand, within BBR_RS12980 at 682.682 kb on + strand, within BBR_RS12980 at 682.698 kb on - strand, within BBR_RS12980 at 682.700 kb on + strand, within BBR_RS12980 at 682.740 kb on + strand at 682.752 kb on - strand at 682.767 kb on + strand at 682.775 kb on - strand at 682.853 kb on - strand at 682.858 kb on - strand at 682.858 kb on - strand at 682.927 kb on + strand at 682.957 kb on + strand at 682.991 kb on + strand at 683.003 kb on - strand at 683.009 kb on - strand at 683.009 kb on - strand at 683.033 kb on + strand at 683.033 kb on + strand at 683.033 kb on + strand at 683.114 kb on + strand at 683.115 kb on - strand at 683.115 kb on - strand at 683.122 kb on + strand at 683.123 kb on + strand at 683.131 kb on - strand at 683.138 kb on - strand at 683.138 kb on - strand at 683.138 kb on - strand at 683.153 kb on + strand at 683.172 kb on + strand at 683.172 kb on + strand at 683.180 kb on - strand at 683.217 kb on + strand at 683.227 kb on + strand at 683.231 kb on + strand at 683.232 kb on - strand at 683.236 kb on + strand at 683.253 kb on + strand at 683.253 kb on + strand at 683.268 kb on + strand at 683.268 kb on + strand at 683.276 kb on - strand at 683.279 kb on - strand at 683.299 kb on + strand at 683.299 kb on + strand at 683.301 kb on + strand at 683.385 kb on - strand at 683.411 kb on - strand at 683.415 kb on + strand at 683.478 kb on - strand, within BBR_RS12985 at 683.483 kb on + strand, within BBR_RS12985 at 683.503 kb on - strand, within BBR_RS12985 at 683.505 kb on + strand, within BBR_RS12985 at 683.523 kb on - strand, within BBR_RS12985 at 683.543 kb on + strand, within BBR_RS12985 at 683.604 kb on - strand, within BBR_RS12985 at 683.607 kb on + strand, within BBR_RS12985 at 683.610 kb on - strand, within BBR_RS12985 at 683.614 kb on + strand, within BBR_RS12985 at 683.663 kb on + strand, within BBR_RS12985 at 683.682 kb on - strand, within BBR_RS12985 at 683.714 kb on - strand, within BBR_RS12985 at 683.754 kb on - strand, within BBR_RS12985 at 683.771 kb on - strand, within BBR_RS12985 at 683.881 kb on + strand, within BBR_RS12985 at 683.917 kb on + strand, within BBR_RS12985 at 683.941 kb on - strand, within BBR_RS12985 at 683.965 kb on - strand, within BBR_RS12985 at 683.975 kb on - strand, within BBR_RS12985 at 684.010 kb on + strand, within BBR_RS12985 at 684.027 kb on - strand, within BBR_RS12985 at 684.039 kb on - strand, within BBR_RS12985 at 684.053 kb on + strand, within BBR_RS12985 at 684.059 kb on + strand, within BBR_RS12985 at 684.073 kb on - strand, within BBR_RS12985 at 684.074 kb on + strand, within BBR_RS12985 at 684.108 kb on + strand, within BBR_RS12985 at 684.116 kb on - strand, within BBR_RS12985 at 684.133 kb on + strand, within BBR_RS12985 at 684.147 kb on + strand, within BBR_RS12985 at 684.155 kb on - strand, within BBR_RS12985 at 684.165 kb on + strand, within BBR_RS12985 at 684.204 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.341 kb on - strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.431 kb on - strand, within BBR_RS12985 at 684.433 kb on + strand, within BBR_RS12985 at 684.441 kb on - strand, within BBR_RS12985 at 684.468 kb on - strand, within BBR_RS12985 at 684.497 kb on - strand at 684.497 kb on - strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.552 kb on + strand at 684.558 kb on - strand at 684.573 kb on - strand at 684.584 kb on - strand at 684.599 kb on + strand at 684.599 kb on + strand at 684.607 kb on - strand at 684.607 kb on - strand at 684.694 kb on + strand at 684.735 kb on + strand at 684.749 kb on + strand at 684.749 kb on + strand at 684.803 kb on + strand, within BBR_RS12990 at 684.808 kb on + strand, within BBR_RS12990 at 684.831 kb on - strand, within BBR_RS12990 at 684.856 kb on + strand, within BBR_RS12990 at 684.874 kb on - strand, within BBR_RS12990 at 684.879 kb on - strand, within BBR_RS12990 at 684.887 kb on - strand, within BBR_RS12990 at 684.908 kb on - strand, within BBR_RS12990 at 684.925 kb on + strand, within BBR_RS12990 at 684.926 kb on - strand, within BBR_RS12990 at 684.929 kb on - strand, within BBR_RS12990 at 684.952 kb on + strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.990 kb on + strand, within BBR_RS12990 at 684.997 kb on + strand, within BBR_RS12990 at 685.015 kb on - strand, within BBR_RS12990 at 685.077 kb on + strand, within BBR_RS12990 at 685.091 kb on + strand, within BBR_RS12990 at 685.096 kb on + strand, within BBR_RS12990 at 685.105 kb on + strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.123 kb on + strand, within BBR_RS12990 at 685.131 kb on - strand, within BBR_RS12990 at 685.139 kb on - strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.178 kb on - strand, within BBR_RS12990 at 685.229 kb on - strand, within BBR_RS12990 at 685.249 kb on + strand, within BBR_RS12990 at 685.257 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.289 kb on - strand, within BBR_RS12990 at 685.297 kb on - strand, within BBR_RS12990 at 685.409 kb on + strand at 685.412 kb on - strand at 685.412 kb on - strand at 685.434 kb on + strand at 685.456 kb on - strand at 685.517 kb on + strand at 685.520 kb on + strand at 685.541 kb on + strand at 685.549 kb on + strand at 685.549 kb on + strand at 685.551 kb on + strand at 685.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 682,294 - BBR_RS12980 0.79 -1.2 682,310 + BBR_RS12980 0.80 -0.4 682,328 - BBR_RS12980 0.80 +0.1 682,332 - BBR_RS12980 0.80 +0.9 682,342 + BBR_RS12980 0.80 +0.4 682,342 + BBR_RS12980 0.80 -0.4 682,345 - BBR_RS12980 0.80 -1.1 682,347 + BBR_RS12980 0.81 -0.5 682,365 - BBR_RS12980 0.81 -0.7 682,378 + BBR_RS12980 0.81 -1.0 682,396 - BBR_RS12980 0.82 -1.9 682,412 - BBR_RS12980 0.82 +0.1 682,442 - BBR_RS12980 0.83 -1.6 682,469 - BBR_RS12980 0.84 -2.2 682,470 + BBR_RS12980 0.84 -0.3 682,553 + BBR_RS12980 0.86 +0.4 682,600 - BBR_RS12980 0.87 -1.0 682,608 - BBR_RS12980 0.87 -0.3 682,624 - BBR_RS12980 0.88 -0.6 682,661 + BBR_RS12980 0.88 +0.1 682,674 - BBR_RS12980 0.89 -0.3 682,682 + BBR_RS12980 0.89 -0.5 682,698 - BBR_RS12980 0.89 +0.5 682,700 + BBR_RS12980 0.89 +1.2 682,740 + +0.1 682,752 - -0.9 682,767 + +0.7 682,775 - +0.5 682,853 - +1.5 682,858 - -0.6 682,858 - -1.5 682,927 + -0.3 682,957 + +0.9 682,991 + +0.9 683,003 - -1.3 683,009 - +0.3 683,009 - -0.1 683,033 + +0.2 683,033 + -0.3 683,033 + -1.0 683,114 + -0.6 683,115 - -0.2 683,115 - +0.1 683,122 + +0.6 683,123 + +0.2 683,131 - +0.2 683,138 - +0.2 683,138 - +0.1 683,138 - +0.0 683,153 + +0.0 683,172 + +0.3 683,172 + -0.5 683,180 - +0.3 683,217 + -0.2 683,227 + -0.7 683,231 + +0.5 683,232 - -0.1 683,236 + -0.0 683,253 + -0.1 683,253 + +0.2 683,268 + +0.3 683,268 + +0.7 683,276 - -0.6 683,279 - +0.3 683,299 + -1.2 683,299 + -1.0 683,301 + -0.5 683,385 - +0.0 683,411 - -1.2 683,415 + +0.1 683,478 - BBR_RS12985 0.15 -1.3 683,483 + BBR_RS12985 0.15 +0.1 683,503 - BBR_RS12985 0.17 +0.3 683,505 + BBR_RS12985 0.17 -1.1 683,523 - BBR_RS12985 0.18 -1.8 683,543 + BBR_RS12985 0.20 +0.1 683,604 - BBR_RS12985 0.24 +0.1 683,607 + BBR_RS12985 0.24 +1.6 683,610 - BBR_RS12985 0.25 -0.6 683,614 + BBR_RS12985 0.25 -1.6 683,663 + BBR_RS12985 0.29 -0.1 683,682 - BBR_RS12985 0.30 -0.6 683,714 - BBR_RS12985 0.33 -0.9 683,754 - BBR_RS12985 0.36 -0.6 683,771 - BBR_RS12985 0.37 -1.6 683,881 + BBR_RS12985 0.45 -1.0 683,917 + BBR_RS12985 0.48 -0.4 683,941 - BBR_RS12985 0.50 -1.6 683,965 - BBR_RS12985 0.52 -1.1 683,975 - BBR_RS12985 0.52 +0.0 684,010 + BBR_RS12985 0.55 -0.5 684,027 - BBR_RS12985 0.56 -0.7 684,039 - BBR_RS12985 0.57 -2.2 684,053 + BBR_RS12985 0.58 +0.3 684,059 + BBR_RS12985 0.59 -0.5 684,073 - BBR_RS12985 0.60 +1.6 684,074 + BBR_RS12985 0.60 -1.1 684,108 + BBR_RS12985 0.62 -0.4 684,116 - BBR_RS12985 0.63 +0.3 684,133 + BBR_RS12985 0.64 +0.7 684,147 + BBR_RS12985 0.65 +0.6 684,155 - BBR_RS12985 0.66 -0.1 684,165 + BBR_RS12985 0.67 -0.6 684,204 + BBR_RS12985 0.70 -0.3 684,283 + BBR_RS12985 0.76 -0.3 684,283 + BBR_RS12985 0.76 -0.5 684,323 - BBR_RS12985 0.79 +0.7 684,323 - BBR_RS12985 0.79 +0.5 684,341 - BBR_RS12985 0.80 -0.7 684,428 + BBR_RS12985 0.87 -0.1 684,428 + BBR_RS12985 0.87 +0.0 684,428 + BBR_RS12985 0.87 -0.5 684,431 - BBR_RS12985 0.87 -0.4 684,433 + BBR_RS12985 0.87 +0.0 684,441 - BBR_RS12985 0.88 -0.8 684,468 - BBR_RS12985 0.90 -1.8 684,497 - -1.1 684,497 - -0.5 684,550 + +2.3 684,550 + +0.4 684,550 + -0.3 684,552 + +0.1 684,558 - -0.1 684,573 - -1.0 684,584 - -1.1 684,599 + -0.1 684,599 + +0.5 684,607 - +0.6 684,607 - -0.5 684,694 + -0.2 684,735 + +1.0 684,749 + +1.7 684,749 + +0.5 684,803 + BBR_RS12990 0.15 +1.0 684,808 + BBR_RS12990 0.15 +0.3 684,831 - BBR_RS12990 0.18 +0.1 684,856 + BBR_RS12990 0.22 -0.6 684,874 - BBR_RS12990 0.24 -1.0 684,879 - BBR_RS12990 0.25 +0.4 684,887 - BBR_RS12990 0.26 -0.1 684,908 - BBR_RS12990 0.29 +0.5 684,925 + BBR_RS12990 0.31 -1.1 684,926 - BBR_RS12990 0.31 -0.2 684,929 - BBR_RS12990 0.31 +0.6 684,952 + BBR_RS12990 0.34 -0.3 684,960 - BBR_RS12990 0.35 +0.2 684,960 - BBR_RS12990 0.35 +0.2 684,960 - BBR_RS12990 0.35 +0.9 684,960 - BBR_RS12990 0.35 -0.2 684,990 + BBR_RS12990 0.39 -0.3 684,997 + BBR_RS12990 0.40 +0.2 685,015 - BBR_RS12990 0.43 -0.1 685,077 + BBR_RS12990 0.51 -1.0 685,091 + BBR_RS12990 0.53 -0.7 685,096 + BBR_RS12990 0.53 -0.8 685,105 + BBR_RS12990 0.55 +1.0 685,113 - BBR_RS12990 0.56 +0.7 685,113 - BBR_RS12990 0.56 -0.1 685,123 + BBR_RS12990 0.57 +0.3 685,131 - BBR_RS12990 0.58 +0.1 685,139 - BBR_RS12990 0.59 +0.5 685,145 + BBR_RS12990 0.60 +0.7 685,145 + BBR_RS12990 0.60 +0.6 685,145 + BBR_RS12990 0.60 -0.7 685,145 + BBR_RS12990 0.60 +0.1 685,145 + BBR_RS12990 0.60 -0.5 685,153 - BBR_RS12990 0.61 -0.8 685,153 - BBR_RS12990 0.61 -0.1 685,153 - BBR_RS12990 0.61 +0.5 685,178 - BBR_RS12990 0.64 -0.7 685,229 - BBR_RS12990 0.71 +0.6 685,249 + BBR_RS12990 0.74 +0.9 685,257 - BBR_RS12990 0.75 -0.4 685,280 - BBR_RS12990 0.78 +0.3 685,280 - BBR_RS12990 0.78 +0.2 685,289 - BBR_RS12990 0.79 -0.0 685,297 - BBR_RS12990 0.80 +0.0 685,409 + -0.4 685,412 - +0.1 685,412 - -0.1 685,434 + -0.1 685,456 - +0.4 685,517 + +0.4 685,520 + -0.5 685,541 + -0.4 685,549 + +0.5 685,549 + -0.3 685,551 + -0.6 685,557 - -1.5
Or see this region's nucleotide sequence