Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12895 and BBR_RS12900 are separated by 305 nucleotides BBR_RS12900 and BBR_RS12905 are separated by 5 nucleotides
BBR_RS12895: BBR_RS12895 - quinone-dependent dihydroorotate dehydrogenase, at 644,932 to 646,083
_RS12895
BBR_RS12900: BBR_RS12900 - DeoR/GlpR transcriptional regulator, at 646,389 to 647,192
_RS12900
BBR_RS12905: BBR_RS12905 - galactose-1-phosphate uridylyltransferase, at 647,198 to 648,448
_RS12905
Position (kb) 646
647
648 Strain fitness (log2 ratio) -1
0
1 at 645.411 kb on - strand, within BBR_RS12895 at 645.444 kb on - strand, within BBR_RS12895 at 645.512 kb on + strand, within BBR_RS12895 at 645.539 kb on + strand, within BBR_RS12895 at 645.596 kb on + strand, within BBR_RS12895 at 645.604 kb on - strand, within BBR_RS12895 at 645.609 kb on - strand, within BBR_RS12895 at 645.609 kb on - strand, within BBR_RS12895 at 645.646 kb on + strand, within BBR_RS12895 at 645.698 kb on + strand, within BBR_RS12895 at 645.706 kb on - strand, within BBR_RS12895 at 645.715 kb on + strand, within BBR_RS12895 at 645.723 kb on - strand, within BBR_RS12895 at 645.726 kb on + strand, within BBR_RS12895 at 645.734 kb on - strand, within BBR_RS12895 at 645.770 kb on - strand, within BBR_RS12895 at 645.875 kb on + strand, within BBR_RS12895 at 645.875 kb on + strand, within BBR_RS12895 at 645.875 kb on + strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.885 kb on - strand, within BBR_RS12895 at 645.907 kb on - strand, within BBR_RS12895 at 645.911 kb on + strand, within BBR_RS12895 at 645.914 kb on - strand, within BBR_RS12895 at 645.927 kb on - strand, within BBR_RS12895 at 646.013 kb on - strand at 646.026 kb on + strand at 646.034 kb on - strand at 646.034 kb on - strand at 646.090 kb on - strand at 646.111 kb on + strand at 646.173 kb on - strand at 646.173 kb on - strand at 646.177 kb on - strand at 646.219 kb on - strand at 646.234 kb on + strand at 646.308 kb on - strand at 646.308 kb on - strand at 646.592 kb on - strand, within BBR_RS12900 at 646.962 kb on + strand, within BBR_RS12900 at 647.041 kb on + strand, within BBR_RS12900 at 647.049 kb on + strand, within BBR_RS12900 at 647.235 kb on + strand at 647.277 kb on + strand at 647.285 kb on - strand at 647.417 kb on + strand, within BBR_RS12905 at 647.765 kb on - strand, within BBR_RS12905 at 647.955 kb on - strand, within BBR_RS12905 at 648.176 kb on - strand, within BBR_RS12905
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 645,411 - BBR_RS12895 0.42 -0.1 645,444 - BBR_RS12895 0.44 -0.1 645,512 + BBR_RS12895 0.50 -0.1 645,539 + BBR_RS12895 0.53 -0.0 645,596 + BBR_RS12895 0.58 -1.0 645,604 - BBR_RS12895 0.58 +0.3 645,609 - BBR_RS12895 0.59 -0.8 645,609 - BBR_RS12895 0.59 -0.5 645,646 + BBR_RS12895 0.62 +0.0 645,698 + BBR_RS12895 0.66 +0.5 645,706 - BBR_RS12895 0.67 -0.1 645,715 + BBR_RS12895 0.68 -0.3 645,723 - BBR_RS12895 0.69 +0.7 645,726 + BBR_RS12895 0.69 +0.1 645,734 - BBR_RS12895 0.70 -0.0 645,770 - BBR_RS12895 0.73 -0.8 645,875 + BBR_RS12895 0.82 -1.1 645,875 + BBR_RS12895 0.82 -0.4 645,875 + BBR_RS12895 0.82 +0.9 645,883 - BBR_RS12895 0.83 -0.0 645,883 - BBR_RS12895 0.83 +0.2 645,883 - BBR_RS12895 0.83 +0.0 645,883 - BBR_RS12895 0.83 +0.3 645,885 - BBR_RS12895 0.83 -0.8 645,907 - BBR_RS12895 0.85 -0.6 645,911 + BBR_RS12895 0.85 +0.5 645,914 - BBR_RS12895 0.85 +0.2 645,927 - BBR_RS12895 0.86 +0.2 646,013 - +1.5 646,026 + +0.1 646,034 - +0.6 646,034 - -0.2 646,090 - +0.5 646,111 + -0.2 646,173 - +0.1 646,173 - -1.2 646,177 - -0.9 646,219 - -1.2 646,234 + -0.0 646,308 - -0.0 646,308 - +0.6 646,592 - BBR_RS12900 0.25 -0.2 646,962 + BBR_RS12900 0.71 -1.2 647,041 + BBR_RS12900 0.81 -0.4 647,049 + BBR_RS12900 0.82 -1.2 647,235 + -0.2 647,277 + -0.9 647,285 - -0.6 647,417 + BBR_RS12905 0.18 -0.9 647,765 - BBR_RS12905 0.45 -0.2 647,955 - BBR_RS12905 0.61 +0.5 648,176 - BBR_RS12905 0.78 -0.7
Or see this region's nucleotide sequence