Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12815 and BBR_RS12820 are separated by 301 nucleotides BBR_RS12820 and BBR_RS12825 are separated by 197 nucleotides
BBR_RS12815: BBR_RS12815 - hypothetical protein, at 624,485 to 624,919
_RS12815
BBR_RS12820: BBR_RS12820 - hypothetical protein, at 625,221 to 626,657
_RS12820
BBR_RS12825: BBR_RS12825 - hypothetical protein, at 626,855 to 628,222
_RS12825
Position (kb)
625
626
627 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 624.227 kb on + strand at 624.233 kb on - strand at 624.233 kb on - strand at 624.243 kb on - strand at 624.257 kb on - strand at 624.283 kb on - strand at 624.285 kb on + strand at 624.293 kb on - strand at 624.361 kb on + strand at 624.369 kb on - strand at 624.369 kb on - strand at 624.373 kb on + strand at 624.455 kb on + strand at 624.455 kb on + strand at 624.455 kb on - strand at 624.492 kb on + strand at 624.493 kb on - strand at 624.519 kb on - strand at 624.519 kb on - strand at 624.520 kb on - strand at 624.528 kb on - strand at 624.557 kb on + strand, within BBR_RS12815 at 624.571 kb on + strand, within BBR_RS12815 at 624.571 kb on + strand, within BBR_RS12815 at 624.599 kb on + strand, within BBR_RS12815 at 624.632 kb on - strand, within BBR_RS12815 at 624.670 kb on + strand, within BBR_RS12815 at 624.672 kb on - strand, within BBR_RS12815 at 624.693 kb on + strand, within BBR_RS12815 at 624.696 kb on - strand, within BBR_RS12815 at 624.700 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.717 kb on + strand, within BBR_RS12815 at 624.725 kb on - strand, within BBR_RS12815 at 624.735 kb on - strand, within BBR_RS12815 at 624.761 kb on + strand, within BBR_RS12815 at 624.788 kb on - strand, within BBR_RS12815 at 624.852 kb on - strand, within BBR_RS12815 at 624.859 kb on - strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.881 kb on - strand at 624.881 kb on - strand at 624.899 kb on - strand at 624.928 kb on + strand at 624.936 kb on - strand at 624.936 kb on - strand at 625.094 kb on + strand at 625.095 kb on - strand at 625.095 kb on - strand at 625.100 kb on - strand at 625.117 kb on - strand at 625.137 kb on + strand at 625.148 kb on + strand at 625.168 kb on + strand at 625.168 kb on + strand at 625.221 kb on - strand at 625.257 kb on + strand at 625.347 kb on + strand at 625.353 kb on - strand at 625.400 kb on + strand, within BBR_RS12820 at 625.401 kb on + strand, within BBR_RS12820 at 625.447 kb on + strand, within BBR_RS12820 at 625.475 kb on + strand, within BBR_RS12820 at 625.505 kb on + strand, within BBR_RS12820 at 625.560 kb on - strand, within BBR_RS12820 at 625.662 kb on - strand, within BBR_RS12820 at 625.705 kb on + strand, within BBR_RS12820 at 625.708 kb on - strand, within BBR_RS12820 at 625.727 kb on - strand, within BBR_RS12820 at 625.727 kb on - strand, within BBR_RS12820 at 625.734 kb on + strand, within BBR_RS12820 at 625.734 kb on + strand, within BBR_RS12820 at 625.760 kb on + strand, within BBR_RS12820 at 625.773 kb on + strand, within BBR_RS12820 at 625.798 kb on - strand, within BBR_RS12820 at 625.806 kb on - strand, within BBR_RS12820 at 625.812 kb on - strand, within BBR_RS12820 at 625.831 kb on + strand, within BBR_RS12820 at 625.831 kb on + strand, within BBR_RS12820 at 625.876 kb on + strand, within BBR_RS12820 at 625.883 kb on + strand, within BBR_RS12820 at 626.018 kb on + strand, within BBR_RS12820 at 626.020 kb on + strand, within BBR_RS12820 at 626.020 kb on + strand, within BBR_RS12820 at 626.020 kb on + strand, within BBR_RS12820 at 626.044 kb on + strand, within BBR_RS12820 at 626.047 kb on + strand, within BBR_RS12820 at 626.047 kb on + strand, within BBR_RS12820 at 626.047 kb on + strand, within BBR_RS12820 at 626.047 kb on + strand, within BBR_RS12820 at 626.055 kb on - strand, within BBR_RS12820 at 626.147 kb on + strand, within BBR_RS12820 at 626.195 kb on + strand, within BBR_RS12820 at 626.197 kb on + strand, within BBR_RS12820 at 626.203 kb on - strand, within BBR_RS12820 at 626.219 kb on + strand, within BBR_RS12820 at 626.240 kb on + strand, within BBR_RS12820 at 626.258 kb on + strand, within BBR_RS12820 at 626.294 kb on + strand, within BBR_RS12820 at 626.344 kb on + strand, within BBR_RS12820 at 626.344 kb on + strand, within BBR_RS12820 at 626.411 kb on + strand, within BBR_RS12820 at 626.430 kb on + strand, within BBR_RS12820 at 626.448 kb on + strand, within BBR_RS12820 at 626.496 kb on + strand, within BBR_RS12820 at 626.496 kb on + strand, within BBR_RS12820 at 626.503 kb on + strand, within BBR_RS12820 at 626.509 kb on - strand, within BBR_RS12820 at 626.549 kb on + strand at 626.619 kb on - strand at 626.622 kb on + strand at 626.667 kb on - strand at 626.703 kb on - strand at 626.711 kb on - strand at 626.717 kb on + strand at 626.717 kb on + strand at 626.718 kb on + strand at 626.806 kb on + strand at 626.806 kb on + strand at 626.821 kb on + strand at 626.824 kb on - strand at 626.824 kb on - strand at 626.829 kb on - strand at 626.829 kb on - strand at 626.886 kb on + strand at 626.964 kb on + strand at 626.964 kb on + strand at 626.967 kb on - strand at 627.011 kb on - strand, within BBR_RS12825 at 627.029 kb on - strand, within BBR_RS12825 at 627.037 kb on + strand, within BBR_RS12825 at 627.077 kb on + strand, within BBR_RS12825 at 627.077 kb on + strand, within BBR_RS12825 at 627.085 kb on - strand, within BBR_RS12825 at 627.095 kb on - strand, within BBR_RS12825 at 627.163 kb on - strand, within BBR_RS12825 at 627.170 kb on - strand, within BBR_RS12825 at 627.316 kb on + strand, within BBR_RS12825 at 627.316 kb on + strand, within BBR_RS12825 at 627.332 kb on - strand, within BBR_RS12825 at 627.356 kb on - strand, within BBR_RS12825 at 627.360 kb on + strand, within BBR_RS12825 at 627.435 kb on - strand, within BBR_RS12825 at 627.460 kb on + strand, within BBR_RS12825 at 627.468 kb on - strand, within BBR_RS12825 at 627.514 kb on + strand, within BBR_RS12825 at 627.554 kb on + strand, within BBR_RS12825 at 627.568 kb on + strand, within BBR_RS12825 at 627.568 kb on + strand, within BBR_RS12825 at 627.575 kb on + strand, within BBR_RS12825 at 627.594 kb on - strand, within BBR_RS12825 at 627.594 kb on - strand, within BBR_RS12825 at 627.596 kb on + strand, within BBR_RS12825 at 627.604 kb on + strand, within BBR_RS12825 at 627.612 kb on - strand, within BBR_RS12825 at 627.612 kb on - strand, within BBR_RS12825 at 627.622 kb on + strand, within BBR_RS12825 at 627.635 kb on + strand, within BBR_RS12825 at 627.643 kb on - strand, within BBR_RS12825
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 624,227 + +0.5 624,233 - +0.8 624,233 - -0.0 624,243 - +1.2 624,257 - +0.6 624,283 - -0.0 624,285 + -0.3 624,293 - +0.2 624,361 + -0.0 624,369 - +0.3 624,369 - +0.2 624,373 + +0.7 624,455 + -0.1 624,455 + +0.4 624,455 - +0.7 624,492 + +0.2 624,493 - +0.5 624,519 - -0.4 624,519 - +0.2 624,520 - -0.4 624,528 - -1.0 624,557 + BBR_RS12815 0.17 -0.3 624,571 + BBR_RS12815 0.20 +0.1 624,571 + BBR_RS12815 0.20 -0.2 624,599 + BBR_RS12815 0.26 +0.7 624,632 - BBR_RS12815 0.34 +0.4 624,670 + BBR_RS12815 0.43 +0.3 624,672 - BBR_RS12815 0.43 +0.2 624,693 + BBR_RS12815 0.48 -0.2 624,696 - BBR_RS12815 0.49 +0.5 624,700 - BBR_RS12815 0.49 -0.1 624,701 - BBR_RS12815 0.50 +0.0 624,701 - BBR_RS12815 0.50 +0.1 624,701 - BBR_RS12815 0.50 +0.3 624,717 + BBR_RS12815 0.53 +0.4 624,725 - BBR_RS12815 0.55 +0.2 624,735 - BBR_RS12815 0.57 -1.1 624,761 + BBR_RS12815 0.63 +1.9 624,788 - BBR_RS12815 0.70 +0.8 624,852 - BBR_RS12815 0.84 +0.8 624,859 - BBR_RS12815 0.86 -0.4 624,873 + BBR_RS12815 0.89 +0.3 624,873 + BBR_RS12815 0.89 +0.5 624,873 + BBR_RS12815 0.89 +1.1 624,881 - +0.5 624,881 - -0.3 624,899 - -0.9 624,928 + +0.1 624,936 - +0.2 624,936 - +0.0 625,094 + -0.7 625,095 - +0.0 625,095 - -0.1 625,100 - +0.3 625,117 - -0.5 625,137 + +0.0 625,148 + +0.4 625,168 + +0.3 625,168 + -0.6 625,221 - +0.0 625,257 + -0.2 625,347 + -0.9 625,353 - -1.5 625,400 + BBR_RS12820 0.12 -1.3 625,401 + BBR_RS12820 0.13 -1.4 625,447 + BBR_RS12820 0.16 -1.9 625,475 + BBR_RS12820 0.18 -0.3 625,505 + BBR_RS12820 0.20 -0.2 625,560 - BBR_RS12820 0.24 -0.3 625,662 - BBR_RS12820 0.31 -0.3 625,705 + BBR_RS12820 0.34 -1.0 625,708 - BBR_RS12820 0.34 +0.5 625,727 - BBR_RS12820 0.35 -2.2 625,727 - BBR_RS12820 0.35 -0.1 625,734 + BBR_RS12820 0.36 -1.1 625,734 + BBR_RS12820 0.36 -1.0 625,760 + BBR_RS12820 0.38 -0.7 625,773 + BBR_RS12820 0.38 -0.8 625,798 - BBR_RS12820 0.40 -2.0 625,806 - BBR_RS12820 0.41 -0.4 625,812 - BBR_RS12820 0.41 -1.2 625,831 + BBR_RS12820 0.42 -0.4 625,831 + BBR_RS12820 0.42 -0.0 625,876 + BBR_RS12820 0.46 -2.2 625,883 + BBR_RS12820 0.46 -0.7 626,018 + BBR_RS12820 0.55 -0.6 626,020 + BBR_RS12820 0.56 -0.4 626,020 + BBR_RS12820 0.56 -0.9 626,020 + BBR_RS12820 0.56 +0.3 626,044 + BBR_RS12820 0.57 -0.6 626,047 + BBR_RS12820 0.57 -1.1 626,047 + BBR_RS12820 0.57 -0.9 626,047 + BBR_RS12820 0.57 -1.2 626,047 + BBR_RS12820 0.57 -0.6 626,055 - BBR_RS12820 0.58 -1.2 626,147 + BBR_RS12820 0.64 -1.6 626,195 + BBR_RS12820 0.68 -1.2 626,197 + BBR_RS12820 0.68 -0.1 626,203 - BBR_RS12820 0.68 -1.3 626,219 + BBR_RS12820 0.69 -0.6 626,240 + BBR_RS12820 0.71 -1.9 626,258 + BBR_RS12820 0.72 -1.1 626,294 + BBR_RS12820 0.75 -0.8 626,344 + BBR_RS12820 0.78 -1.2 626,344 + BBR_RS12820 0.78 -0.1 626,411 + BBR_RS12820 0.83 -1.8 626,430 + BBR_RS12820 0.84 -2.0 626,448 + BBR_RS12820 0.85 -0.4 626,496 + BBR_RS12820 0.89 -0.6 626,496 + BBR_RS12820 0.89 -0.1 626,503 + BBR_RS12820 0.89 -0.6 626,509 - BBR_RS12820 0.90 -0.6 626,549 + +0.1 626,619 - +1.9 626,622 + +0.1 626,667 - +0.5 626,703 - +0.7 626,711 - -0.1 626,717 + +0.3 626,717 + -0.2 626,718 + +0.5 626,806 + +0.6 626,806 + +0.5 626,821 + -0.2 626,824 - +0.3 626,824 - +0.3 626,829 - +0.1 626,829 - -0.2 626,886 + +0.5 626,964 + +0.5 626,964 + +0.4 626,967 - -0.7 627,011 - BBR_RS12825 0.11 +0.2 627,029 - BBR_RS12825 0.13 -0.1 627,037 + BBR_RS12825 0.13 +0.8 627,077 + BBR_RS12825 0.16 +0.0 627,077 + BBR_RS12825 0.16 +0.3 627,085 - BBR_RS12825 0.17 -0.0 627,095 - BBR_RS12825 0.18 -0.4 627,163 - BBR_RS12825 0.23 +0.6 627,170 - BBR_RS12825 0.23 +0.0 627,316 + BBR_RS12825 0.34 +0.4 627,316 + BBR_RS12825 0.34 +0.7 627,332 - BBR_RS12825 0.35 +0.3 627,356 - BBR_RS12825 0.37 -0.7 627,360 + BBR_RS12825 0.37 +1.1 627,435 - BBR_RS12825 0.42 +0.2 627,460 + BBR_RS12825 0.44 +0.3 627,468 - BBR_RS12825 0.45 -0.3 627,514 + BBR_RS12825 0.48 +0.3 627,554 + BBR_RS12825 0.51 -0.6 627,568 + BBR_RS12825 0.52 +0.5 627,568 + BBR_RS12825 0.52 +0.7 627,575 + BBR_RS12825 0.53 +0.6 627,594 - BBR_RS12825 0.54 -0.4 627,594 - BBR_RS12825 0.54 -1.1 627,596 + BBR_RS12825 0.54 -0.3 627,604 + BBR_RS12825 0.55 +0.2 627,612 - BBR_RS12825 0.55 +0.1 627,612 - BBR_RS12825 0.55 -1.6 627,622 + BBR_RS12825 0.56 -0.6 627,635 + BBR_RS12825 0.57 +0.8 627,643 - BBR_RS12825 0.58 -0.1
Or see this region's nucleotide sequence