Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12605 and BBR_RS12610 overlap by 4 nucleotides BBR_RS12610 and BBR_RS20720 overlap by 14 nucleotides BBR_RS20720 and BBR_RS12620 are separated by 27 nucleotides
BBR_RS12600: BBR_RS12600 - serine acetyltransferase, at 583,628 to 584,092
_RS12600
BBR_RS12605: BBR_RS12605 - glycosyltransferase family 2 protein, at 584,085 to 585,095
_RS12605
BBR_RS12610: BBR_RS12610 - capsular polysaccharide biosynthesis protein, at 585,092 to 586,027
_RS12610
BBR_RS20720: BBR_RS20720 - DUF4422 domain-containing protein, at 586,014 to 586,844
_RS20720
BBR_RS12620: BBR_RS12620 - glycosyltransferase family 1 protein, at 586,872 to 587,945
_RS12620
Position (kb) 585
586
587 Strain fitness (log2 ratio) -2
-1
0
1 at 584.150 kb on + strand at 584.171 kb on - strand at 584.201 kb on - strand, within BBR_RS12605 at 584.441 kb on - strand, within BBR_RS12605 at 584.474 kb on + strand, within BBR_RS12605 at 584.482 kb on - strand, within BBR_RS12605 at 584.482 kb on - strand, within BBR_RS12605 at 584.504 kb on + strand, within BBR_RS12605 at 584.565 kb on - strand, within BBR_RS12605 at 584.742 kb on - strand, within BBR_RS12605 at 585.106 kb on + strand at 585.197 kb on - strand, within BBR_RS12610 at 585.216 kb on - strand, within BBR_RS12610 at 585.274 kb on - strand, within BBR_RS12610 at 585.282 kb on - strand, within BBR_RS12610 at 585.342 kb on + strand, within BBR_RS12610 at 585.355 kb on - strand, within BBR_RS12610 at 585.434 kb on + strand, within BBR_RS12610 at 585.505 kb on + strand, within BBR_RS12610 at 585.605 kb on - strand, within BBR_RS12610 at 585.672 kb on - strand, within BBR_RS12610 at 585.769 kb on - strand, within BBR_RS12610 at 585.778 kb on + strand, within BBR_RS12610 at 585.786 kb on - strand, within BBR_RS12610 at 585.832 kb on + strand, within BBR_RS12610 at 585.896 kb on - strand, within BBR_RS12610 at 585.953 kb on - strand at 585.953 kb on - strand at 585.973 kb on - strand at 585.981 kb on + strand at 585.989 kb on - strand at 585.989 kb on - strand at 585.991 kb on - strand at 586.002 kb on + strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.043 kb on - strand at 586.056 kb on - strand at 586.061 kb on + strand at 586.083 kb on - strand at 586.101 kb on - strand, within BBR_RS20720 at 586.153 kb on + strand, within BBR_RS20720 at 586.153 kb on + strand, within BBR_RS20720 at 586.161 kb on - strand, within BBR_RS20720 at 586.171 kb on + strand, within BBR_RS20720 at 586.240 kb on - strand, within BBR_RS20720 at 586.251 kb on - strand, within BBR_RS20720 at 586.272 kb on - strand, within BBR_RS20720 at 586.366 kb on + strand, within BBR_RS20720 at 586.458 kb on - strand, within BBR_RS20720 at 586.458 kb on - strand, within BBR_RS20720 at 586.502 kb on + strand, within BBR_RS20720 at 586.597 kb on + strand, within BBR_RS20720 at 586.636 kb on - strand, within BBR_RS20720 at 586.636 kb on - strand, within BBR_RS20720 at 586.679 kb on - strand, within BBR_RS20720 at 586.789 kb on + strand at 586.789 kb on - strand at 586.819 kb on - strand at 586.819 kb on - strand at 586.855 kb on - strand at 586.888 kb on + strand at 586.888 kb on + strand at 586.896 kb on - strand at 586.901 kb on - strand at 586.906 kb on + strand at 586.906 kb on - strand at 586.908 kb on + strand at 586.916 kb on - strand at 586.921 kb on - strand at 586.924 kb on - strand at 586.951 kb on + strand at 586.959 kb on - strand at 586.969 kb on - strand at 586.969 kb on - strand at 586.982 kb on - strand, within BBR_RS12620 at 586.983 kb on - strand, within BBR_RS12620 at 586.983 kb on - strand, within BBR_RS12620 at 587.001 kb on - strand, within BBR_RS12620
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 584,150 + -2.5 584,171 - -0.4 584,201 - BBR_RS12605 0.11 -0.2 584,441 - BBR_RS12605 0.35 -0.3 584,474 + BBR_RS12605 0.38 -0.5 584,482 - BBR_RS12605 0.39 -0.1 584,482 - BBR_RS12605 0.39 -0.6 584,504 + BBR_RS12605 0.41 -1.0 584,565 - BBR_RS12605 0.47 -1.5 584,742 - BBR_RS12605 0.65 +1.4 585,106 + +0.8 585,197 - BBR_RS12610 0.11 -0.0 585,216 - BBR_RS12610 0.13 +0.0 585,274 - BBR_RS12610 0.19 -0.7 585,282 - BBR_RS12610 0.20 -1.0 585,342 + BBR_RS12610 0.27 -1.3 585,355 - BBR_RS12610 0.28 +0.0 585,434 + BBR_RS12610 0.37 -1.1 585,505 + BBR_RS12610 0.44 +1.6 585,605 - BBR_RS12610 0.55 -0.5 585,672 - BBR_RS12610 0.62 -0.1 585,769 - BBR_RS12610 0.72 -0.3 585,778 + BBR_RS12610 0.73 +0.2 585,786 - BBR_RS12610 0.74 -0.7 585,832 + BBR_RS12610 0.79 -0.7 585,896 - BBR_RS12610 0.86 -0.3 585,953 - -1.0 585,953 - -0.3 585,973 - -1.2 585,981 + -0.9 585,989 - +1.7 585,989 - -2.0 585,991 - -1.2 586,002 + -0.8 586,010 - +0.2 586,010 - +0.2 586,010 - +0.6 586,010 - +0.4 586,010 - +0.2 586,043 - +0.2 586,056 - +1.7 586,061 + +1.6 586,083 - -0.3 586,101 - BBR_RS20720 0.10 -0.6 586,153 + BBR_RS20720 0.17 -0.8 586,153 + BBR_RS20720 0.17 +0.8 586,161 - BBR_RS20720 0.18 -0.9 586,171 + BBR_RS20720 0.19 +0.2 586,240 - BBR_RS20720 0.27 -1.1 586,251 - BBR_RS20720 0.29 -0.3 586,272 - BBR_RS20720 0.31 -0.4 586,366 + BBR_RS20720 0.42 +0.4 586,458 - BBR_RS20720 0.53 -1.2 586,458 - BBR_RS20720 0.53 +0.2 586,502 + BBR_RS20720 0.59 +0.1 586,597 + BBR_RS20720 0.70 -1.7 586,636 - BBR_RS20720 0.75 -0.4 586,636 - BBR_RS20720 0.75 -1.6 586,679 - BBR_RS20720 0.80 -1.4 586,789 + +1.6 586,789 - -0.4 586,819 - -0.5 586,819 - -1.3 586,855 - +0.1 586,888 + -0.8 586,888 + -0.5 586,896 - -0.5 586,901 - -0.7 586,906 + +0.3 586,906 - -0.1 586,908 + -1.2 586,916 - -0.3 586,921 - -0.8 586,924 - -0.6 586,951 + -0.5 586,959 - -0.4 586,969 - -0.6 586,969 - -0.6 586,982 - BBR_RS12620 0.10 +0.0 586,983 - BBR_RS12620 0.10 -0.4 586,983 - BBR_RS12620 0.10 -0.5 587,001 - BBR_RS12620 0.12 +0.2
Or see this region's nucleotide sequence