Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12595 and BBR_RS12600 are separated by 10 nucleotides BBR_RS12600 and BBR_RS12605 overlap by 8 nucleotides BBR_RS12605 and BBR_RS12610 overlap by 4 nucleotides BBR_RS12610 and BBR_RS20720 overlap by 14 nucleotides
BBR_RS12595: BBR_RS12595 - membrane spanning protein, at 582,391 to 583,617
_RS12595
BBR_RS12600: BBR_RS12600 - serine acetyltransferase, at 583,628 to 584,092
_RS12600
BBR_RS12605: BBR_RS12605 - glycosyltransferase family 2 protein, at 584,085 to 585,095
_RS12605
BBR_RS12610: BBR_RS12610 - capsular polysaccharide biosynthesis protein, at 585,092 to 586,027
_RS12610
BBR_RS20720: BBR_RS20720 - DUF4422 domain-containing protein, at 586,014 to 586,844
_RS20720
Position (kb) 584
585
586 Strain fitness (log2 ratio) -2
-1
0
1 at 583.729 kb on - strand, within BBR_RS12600 at 583.741 kb on + strand, within BBR_RS12600 at 583.763 kb on - strand, within BBR_RS12600 at 583.765 kb on - strand, within BBR_RS12600 at 583.767 kb on - strand, within BBR_RS12600 at 583.771 kb on + strand, within BBR_RS12600 at 583.798 kb on + strand, within BBR_RS12600 at 583.814 kb on - strand, within BBR_RS12600 at 583.898 kb on - strand, within BBR_RS12600 at 583.941 kb on - strand, within BBR_RS12600 at 583.980 kb on + strand, within BBR_RS12600 at 583.988 kb on - strand, within BBR_RS12600 at 584.002 kb on - strand, within BBR_RS12600 at 584.025 kb on - strand, within BBR_RS12600 at 584.034 kb on - strand, within BBR_RS12600 at 584.034 kb on - strand, within BBR_RS12600 at 584.090 kb on - strand at 584.090 kb on - strand at 584.150 kb on + strand at 584.171 kb on - strand at 584.201 kb on - strand, within BBR_RS12605 at 584.441 kb on - strand, within BBR_RS12605 at 584.474 kb on + strand, within BBR_RS12605 at 584.482 kb on - strand, within BBR_RS12605 at 584.482 kb on - strand, within BBR_RS12605 at 584.504 kb on + strand, within BBR_RS12605 at 584.565 kb on - strand, within BBR_RS12605 at 584.742 kb on - strand, within BBR_RS12605 at 585.106 kb on + strand at 585.197 kb on - strand, within BBR_RS12610 at 585.216 kb on - strand, within BBR_RS12610 at 585.274 kb on - strand, within BBR_RS12610 at 585.282 kb on - strand, within BBR_RS12610 at 585.342 kb on + strand, within BBR_RS12610 at 585.355 kb on - strand, within BBR_RS12610 at 585.434 kb on + strand, within BBR_RS12610 at 585.505 kb on + strand, within BBR_RS12610 at 585.605 kb on - strand, within BBR_RS12610 at 585.672 kb on - strand, within BBR_RS12610 at 585.769 kb on - strand, within BBR_RS12610 at 585.778 kb on + strand, within BBR_RS12610 at 585.786 kb on - strand, within BBR_RS12610 at 585.832 kb on + strand, within BBR_RS12610 at 585.896 kb on - strand, within BBR_RS12610 at 585.953 kb on - strand at 585.953 kb on - strand at 585.973 kb on - strand at 585.981 kb on + strand at 585.989 kb on - strand at 585.989 kb on - strand at 585.991 kb on - strand at 586.002 kb on + strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.043 kb on - strand at 586.056 kb on - strand at 586.061 kb on + strand at 586.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 583,729 - BBR_RS12600 0.22 +0.4 583,741 + BBR_RS12600 0.24 +0.2 583,763 - BBR_RS12600 0.29 -0.5 583,765 - BBR_RS12600 0.29 +0.2 583,767 - BBR_RS12600 0.30 -1.3 583,771 + BBR_RS12600 0.31 -0.0 583,798 + BBR_RS12600 0.37 -0.7 583,814 - BBR_RS12600 0.40 +0.5 583,898 - BBR_RS12600 0.58 +0.1 583,941 - BBR_RS12600 0.67 -0.1 583,980 + BBR_RS12600 0.76 +0.4 583,988 - BBR_RS12600 0.77 -0.1 584,002 - BBR_RS12600 0.80 +0.1 584,025 - BBR_RS12600 0.85 -1.5 584,034 - BBR_RS12600 0.87 +0.4 584,034 - BBR_RS12600 0.87 +0.2 584,090 - -0.2 584,090 - -0.2 584,150 + -2.5 584,171 - -0.4 584,201 - BBR_RS12605 0.11 -0.2 584,441 - BBR_RS12605 0.35 -0.3 584,474 + BBR_RS12605 0.38 -0.5 584,482 - BBR_RS12605 0.39 -0.1 584,482 - BBR_RS12605 0.39 -0.6 584,504 + BBR_RS12605 0.41 -1.0 584,565 - BBR_RS12605 0.47 -1.5 584,742 - BBR_RS12605 0.65 +1.4 585,106 + +0.8 585,197 - BBR_RS12610 0.11 -0.0 585,216 - BBR_RS12610 0.13 +0.0 585,274 - BBR_RS12610 0.19 -0.7 585,282 - BBR_RS12610 0.20 -1.0 585,342 + BBR_RS12610 0.27 -1.3 585,355 - BBR_RS12610 0.28 +0.0 585,434 + BBR_RS12610 0.37 -1.1 585,505 + BBR_RS12610 0.44 +1.6 585,605 - BBR_RS12610 0.55 -0.5 585,672 - BBR_RS12610 0.62 -0.1 585,769 - BBR_RS12610 0.72 -0.3 585,778 + BBR_RS12610 0.73 +0.2 585,786 - BBR_RS12610 0.74 -0.7 585,832 + BBR_RS12610 0.79 -0.7 585,896 - BBR_RS12610 0.86 -0.3 585,953 - -1.0 585,953 - -0.3 585,973 - -1.2 585,981 + -0.9 585,989 - +1.7 585,989 - -2.0 585,991 - -1.2 586,002 + -0.8 586,010 - +0.2 586,010 - +0.2 586,010 - +0.6 586,010 - +0.4 586,010 - +0.2 586,043 - +0.2 586,056 - +1.7 586,061 + +1.6 586,083 - -0.3
Or see this region's nucleotide sequence