Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS12590

Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS12585 and BBR_RS12590 are separated by 49 nucleotidesBBR_RS12590 and BBR_RS12595 overlap by 4 nucleotides BBR_RS12585: BBR_RS12585 - O-unit flippase, at 579,997 to 581,460 _RS12585 BBR_RS12590: BBR_RS12590 - beta-1,6-N-acetylglucosaminyltransferase, at 581,510 to 582,394 _RS12590 BBR_RS12595: BBR_RS12595 - membrane spanning protein, at 582,391 to 583,617 _RS12595 Position (kb) 581 582 583Strain fitness (log2 ratio) -2 -1 0 1at 581.428 kb on + strandat 581.494 kb on + strandat 581.494 kb on + strandat 581.501 kb on + strandat 581.502 kb on - strandat 581.502 kb on - strandat 581.509 kb on - strandat 581.509 kb on - strandat 581.509 kb on - strandat 581.509 kb on - strandat 581.509 kb on - strandat 581.509 kb on - strandat 581.582 kb on - strandat 581.804 kb on - strand, within BBR_RS12590at 581.804 kb on - strand, within BBR_RS12590at 581.927 kb on - strand, within BBR_RS12590at 581.952 kb on - strand, within BBR_RS12590at 582.206 kb on - strand, within BBR_RS12590at 582.242 kb on - strand, within BBR_RS12590at 582.340 kb on - strandat 582.350 kb on - strandat 582.432 kb on - strandat 582.477 kb on - strandat 582.562 kb on + strand, within BBR_RS12595

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Per-strain Table

Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
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581,428 + -0.3
581,494 + -0.4
581,494 + -0.2
581,501 + +0.3
581,502 - +1.0
581,502 - +1.4
581,509 - -0.2
581,509 - +0.0
581,509 - -0.7
581,509 - -0.5
581,509 - -0.7
581,509 - +0.8
581,582 - -1.2
581,804 - BBR_RS12590 0.33 -1.0
581,804 - BBR_RS12590 0.33 -0.5
581,927 - BBR_RS12590 0.47 -0.6
581,952 - BBR_RS12590 0.50 -0.8
582,206 - BBR_RS12590 0.79 +0.6
582,242 - BBR_RS12590 0.83 +0.4
582,340 - -2.6
582,350 - +0.4
582,432 - +0.1
582,477 - +0.8
582,562 + BBR_RS12595 0.14 +0.0

Or see this region's nucleotide sequence