Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12550 and BBR_RS12555 are separated by 275 nucleotides BBR_RS12555 and BBR_RS12560 are separated by 391 nucleotides
BBR_RS12550: BBR_RS12550 - sugar transferase, at 573,158 to 574,876
_RS12550
BBR_RS12555: BBR_RS12555 - protein-tyrosine-phosphatase, at 575,152 to 575,757
_RS12555
BBR_RS12560: BBR_RS12560 - transposase, at 576,149 to 576,799
_RS12560
Position (kb)
575
576 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 574.155 kb on - strand, within BBR_RS12550 at 574.240 kb on + strand, within BBR_RS12550 at 574.246 kb on - strand, within BBR_RS12550 at 574.287 kb on - strand, within BBR_RS12550 at 574.326 kb on + strand, within BBR_RS12550 at 574.345 kb on + strand, within BBR_RS12550 at 574.353 kb on - strand, within BBR_RS12550 at 574.390 kb on + strand, within BBR_RS12550 at 574.398 kb on - strand, within BBR_RS12550 at 574.398 kb on - strand, within BBR_RS12550 at 574.432 kb on - strand, within BBR_RS12550 at 574.442 kb on - strand, within BBR_RS12550 at 574.442 kb on - strand, within BBR_RS12550 at 574.461 kb on - strand, within BBR_RS12550 at 574.478 kb on + strand, within BBR_RS12550 at 574.481 kb on + strand, within BBR_RS12550 at 574.496 kb on + strand, within BBR_RS12550 at 574.496 kb on - strand, within BBR_RS12550 at 574.501 kb on + strand, within BBR_RS12550 at 574.501 kb on + strand, within BBR_RS12550 at 574.501 kb on + strand, within BBR_RS12550 at 574.509 kb on - strand, within BBR_RS12550 at 574.516 kb on + strand, within BBR_RS12550 at 574.528 kb on - strand, within BBR_RS12550 at 574.529 kb on - strand, within BBR_RS12550 at 574.534 kb on - strand, within BBR_RS12550 at 574.536 kb on - strand, within BBR_RS12550 at 574.536 kb on - strand, within BBR_RS12550 at 574.545 kb on + strand, within BBR_RS12550 at 574.577 kb on - strand, within BBR_RS12550 at 574.603 kb on - strand, within BBR_RS12550 at 574.604 kb on + strand, within BBR_RS12550 at 574.612 kb on - strand, within BBR_RS12550 at 574.642 kb on + strand, within BBR_RS12550 at 574.642 kb on + strand, within BBR_RS12550 at 574.664 kb on + strand, within BBR_RS12550 at 574.706 kb on - strand at 574.745 kb on - strand at 574.785 kb on - strand at 574.785 kb on - strand at 574.787 kb on + strand at 574.804 kb on + strand at 574.806 kb on + strand at 574.806 kb on + strand at 574.806 kb on + strand at 574.807 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.818 kb on + strand at 574.824 kb on - strand at 574.885 kb on + strand at 574.903 kb on - strand at 574.903 kb on - strand at 574.903 kb on - strand at 574.903 kb on - strand at 574.943 kb on + strand at 574.951 kb on - strand at 575.139 kb on - strand at 575.141 kb on + strand at 575.194 kb on + strand at 575.265 kb on + strand, within BBR_RS12555 at 575.265 kb on + strand, within BBR_RS12555 at 575.271 kb on + strand, within BBR_RS12555 at 575.287 kb on + strand, within BBR_RS12555 at 575.287 kb on + strand, within BBR_RS12555 at 575.292 kb on + strand, within BBR_RS12555 at 575.337 kb on + strand, within BBR_RS12555 at 575.349 kb on + strand, within BBR_RS12555 at 575.356 kb on + strand, within BBR_RS12555 at 575.430 kb on + strand, within BBR_RS12555 at 575.430 kb on + strand, within BBR_RS12555 at 575.503 kb on + strand, within BBR_RS12555 at 575.508 kb on + strand, within BBR_RS12555 at 575.523 kb on - strand, within BBR_RS12555 at 575.553 kb on - strand, within BBR_RS12555 at 575.657 kb on - strand, within BBR_RS12555 at 575.672 kb on - strand, within BBR_RS12555 at 575.686 kb on + strand, within BBR_RS12555 at 575.750 kb on - strand at 575.760 kb on - strand at 575.776 kb on + strand at 575.805 kb on + strand at 575.805 kb on + strand at 575.949 kb on - strand at 576.027 kb on + strand at 576.049 kb on + strand at 576.064 kb on + strand at 576.064 kb on - strand at 576.072 kb on - strand at 576.083 kb on + strand at 576.091 kb on - strand at 576.100 kb on + strand at 576.121 kb on + strand at 576.182 kb on + strand at 576.192 kb on + strand at 576.208 kb on - strand at 576.210 kb on - strand at 576.210 kb on - strand at 576.210 kb on - strand at 576.240 kb on - strand, within BBR_RS12560 at 576.324 kb on - strand, within BBR_RS12560 at 576.415 kb on - strand, within BBR_RS12560 at 576.415 kb on - strand, within BBR_RS12560 at 576.415 kb on - strand, within BBR_RS12560 at 576.425 kb on - strand, within BBR_RS12560 at 576.455 kb on + strand, within BBR_RS12560 at 576.466 kb on + strand, within BBR_RS12560 at 576.555 kb on + strand, within BBR_RS12560 at 576.555 kb on + strand, within BBR_RS12560 at 576.577 kb on + strand, within BBR_RS12560 at 576.596 kb on - strand, within BBR_RS12560 at 576.649 kb on - strand, within BBR_RS12560 at 576.676 kb on + strand, within BBR_RS12560 at 576.728 kb on - strand, within BBR_RS12560 at 576.754 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 574,155 - BBR_RS12550 0.58 -0.6 574,240 + BBR_RS12550 0.63 -0.3 574,246 - BBR_RS12550 0.63 -0.2 574,287 - BBR_RS12550 0.66 -0.3 574,326 + BBR_RS12550 0.68 -0.1 574,345 + BBR_RS12550 0.69 +0.1 574,353 - BBR_RS12550 0.70 -1.1 574,390 + BBR_RS12550 0.72 -0.3 574,398 - BBR_RS12550 0.72 -0.5 574,398 - BBR_RS12550 0.72 -0.6 574,432 - BBR_RS12550 0.74 -0.5 574,442 - BBR_RS12550 0.75 -0.3 574,442 - BBR_RS12550 0.75 -0.2 574,461 - BBR_RS12550 0.76 +0.4 574,478 + BBR_RS12550 0.77 +0.0 574,481 + BBR_RS12550 0.77 -1.2 574,496 + BBR_RS12550 0.78 -0.1 574,496 - BBR_RS12550 0.78 -0.6 574,501 + BBR_RS12550 0.78 +0.5 574,501 + BBR_RS12550 0.78 +0.4 574,501 + BBR_RS12550 0.78 +0.4 574,509 - BBR_RS12550 0.79 +0.3 574,516 + BBR_RS12550 0.79 -0.2 574,528 - BBR_RS12550 0.80 -1.1 574,529 - BBR_RS12550 0.80 +0.1 574,534 - BBR_RS12550 0.80 -1.2 574,536 - BBR_RS12550 0.80 +0.0 574,536 - BBR_RS12550 0.80 -0.9 574,545 + BBR_RS12550 0.81 -0.3 574,577 - BBR_RS12550 0.83 -0.8 574,603 - BBR_RS12550 0.84 -0.4 574,604 + BBR_RS12550 0.84 -0.3 574,612 - BBR_RS12550 0.85 +0.2 574,642 + BBR_RS12550 0.86 -0.6 574,642 + BBR_RS12550 0.86 -0.0 574,664 + BBR_RS12550 0.88 -1.5 574,706 - -0.5 574,745 - -0.6 574,785 - +0.1 574,785 - +0.5 574,787 + -0.0 574,804 + -0.2 574,806 + -1.6 574,806 + -0.4 574,806 + -0.2 574,807 - -0.9 574,814 - -0.3 574,814 - -0.8 574,814 - +0.6 574,814 - -0.1 574,814 - +0.4 574,814 - +0.3 574,818 + +0.2 574,824 - +0.7 574,885 + +0.1 574,903 - +0.0 574,903 - -0.3 574,903 - +0.9 574,903 - -0.0 574,943 + +0.0 574,951 - +0.8 575,139 - +0.8 575,141 + +0.1 575,194 + +0.1 575,265 + BBR_RS12555 0.19 -1.8 575,265 + BBR_RS12555 0.19 -0.9 575,271 + BBR_RS12555 0.20 -1.0 575,287 + BBR_RS12555 0.22 +0.3 575,287 + BBR_RS12555 0.22 -0.3 575,292 + BBR_RS12555 0.23 +0.1 575,337 + BBR_RS12555 0.31 +0.2 575,349 + BBR_RS12555 0.33 -1.5 575,356 + BBR_RS12555 0.34 -0.2 575,430 + BBR_RS12555 0.46 -0.2 575,430 + BBR_RS12555 0.46 -0.9 575,503 + BBR_RS12555 0.58 -0.9 575,508 + BBR_RS12555 0.59 -0.4 575,523 - BBR_RS12555 0.61 +0.3 575,553 - BBR_RS12555 0.66 -0.7 575,657 - BBR_RS12555 0.83 -0.8 575,672 - BBR_RS12555 0.86 +0.4 575,686 + BBR_RS12555 0.88 -0.9 575,750 - -0.5 575,760 - -0.2 575,776 + +0.4 575,805 + +0.1 575,805 + +1.0 575,949 - -0.8 576,027 + +0.9 576,049 + -0.6 576,064 + +0.4 576,064 - +0.0 576,072 - +2.1 576,083 + -1.5 576,091 - -0.9 576,100 + -0.2 576,121 + -0.3 576,182 + +0.1 576,192 + +0.2 576,208 - +0.5 576,210 - -0.1 576,210 - +0.5 576,210 - +0.1 576,240 - BBR_RS12560 0.14 +0.2 576,324 - BBR_RS12560 0.27 -0.4 576,415 - BBR_RS12560 0.41 +0.4 576,415 - BBR_RS12560 0.41 -1.1 576,415 - BBR_RS12560 0.41 -0.1 576,425 - BBR_RS12560 0.42 -0.0 576,455 + BBR_RS12560 0.47 +0.3 576,466 + BBR_RS12560 0.49 -0.2 576,555 + BBR_RS12560 0.62 +0.3 576,555 + BBR_RS12560 0.62 +0.5 576,577 + BBR_RS12560 0.66 +0.5 576,596 - BBR_RS12560 0.69 -0.6 576,649 - BBR_RS12560 0.77 -0.9 576,676 + BBR_RS12560 0.81 -1.4 576,728 - BBR_RS12560 0.89 +0.2 576,754 - -0.1
Or see this region's nucleotide sequence