Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12360 and BBR_RS12365 are separated by 89 nucleotides BBR_RS12365 and BBR_RS12370 are separated by 115 nucleotides BBR_RS12370 and BBR_RS12375 are separated by 5 nucleotides BBR_RS12375 and BBR_RS12380 are separated by 104 nucleotides
BBR_RS12360: BBR_RS12360 - 50S ribosomal protein L32, at 525,074 to 525,268
_RS12360
BBR_RS12365: BBR_RS12365 - DUF177 domain-containing protein, at 525,358 to 525,978
_RS12365
BBR_RS12370: BBR_RS12370 - cell division protein, at 526,094 to 526,879
_RS12370
BBR_RS12375: BBR_RS12375 - pantetheine-phosphate adenylyltransferase, at 526,885 to 527,385
_RS12375
BBR_RS12380: BBR_RS12380 - DUF3039 domain-containing protein, at 527,490 to 527,771
_RS12380
Position (kb)
526
527 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 525.379 kb on + strand at 525.382 kb on - strand at 525.388 kb on - strand at 525.394 kb on - strand at 525.456 kb on + strand, within BBR_RS12365 at 525.456 kb on + strand, within BBR_RS12365 at 525.458 kb on - strand, within BBR_RS12365 at 525.461 kb on + strand, within BBR_RS12365 at 525.464 kb on - strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.476 kb on - strand, within BBR_RS12365 at 525.478 kb on - strand, within BBR_RS12365 at 525.480 kb on + strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.502 kb on - strand, within BBR_RS12365 at 525.528 kb on - strand, within BBR_RS12365 at 525.529 kb on - strand, within BBR_RS12365 at 525.533 kb on - strand, within BBR_RS12365 at 525.602 kb on - strand, within BBR_RS12365 at 525.612 kb on - strand, within BBR_RS12365 at 525.662 kb on + strand, within BBR_RS12365 at 525.667 kb on + strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.676 kb on - strand, within BBR_RS12365 at 525.716 kb on + strand, within BBR_RS12365 at 525.721 kb on + strand, within BBR_RS12365 at 525.735 kb on + strand, within BBR_RS12365 at 525.743 kb on - strand, within BBR_RS12365 at 525.844 kb on - strand, within BBR_RS12365 at 525.850 kb on - strand, within BBR_RS12365 at 525.854 kb on - strand, within BBR_RS12365 at 525.862 kb on - strand, within BBR_RS12365 at 525.890 kb on - strand, within BBR_RS12365 at 525.896 kb on + strand, within BBR_RS12365 at 525.905 kb on + strand, within BBR_RS12365 at 525.939 kb on - strand at 526.027 kb on - strand at 526.034 kb on + strand at 526.065 kb on + strand at 526.070 kb on + strand at 526.073 kb on - strand at 526.073 kb on - strand at 526.078 kb on - strand at 526.078 kb on - strand at 526.082 kb on + strand at 526.082 kb on + strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.110 kb on - strand at 526.131 kb on + strand at 526.733 kb on - strand, within BBR_RS12370 at 526.891 kb on - strand at 526.896 kb on - strand at 526.898 kb on + strand at 526.916 kb on + strand at 527.083 kb on - strand, within BBR_RS12375 at 527.125 kb on - strand, within BBR_RS12375 at 527.212 kb on - strand, within BBR_RS12375 at 527.388 kb on + strand at 527.388 kb on - strand at 527.420 kb on - strand at 527.420 kb on - strand at 527.420 kb on - strand at 527.422 kb on + strand at 527.422 kb on + strand at 527.422 kb on + strand at 527.430 kb on - strand at 527.506 kb on + strand at 527.514 kb on + strand at 527.540 kb on + strand, within BBR_RS12380 at 527.558 kb on - strand, within BBR_RS12380 at 527.561 kb on + strand, within BBR_RS12380 at 527.574 kb on - strand, within BBR_RS12380 at 527.629 kb on - strand, within BBR_RS12380 at 527.636 kb on + strand, within BBR_RS12380 at 527.666 kb on + strand, within BBR_RS12380 at 527.671 kb on + strand, within BBR_RS12380 at 527.685 kb on + strand, within BBR_RS12380 at 527.698 kb on - strand, within BBR_RS12380 at 527.704 kb on + strand, within BBR_RS12380 at 527.704 kb on + strand, within BBR_RS12380 at 527.704 kb on + strand, within BBR_RS12380 at 527.712 kb on + strand, within BBR_RS12380 at 527.712 kb on - strand, within BBR_RS12380 at 527.734 kb on - strand, within BBR_RS12380 at 527.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 525,379 + -0.3 525,382 - -1.0 525,388 - -0.5 525,394 - -0.4 525,456 + BBR_RS12365 0.16 +1.1 525,456 + BBR_RS12365 0.16 +0.6 525,458 - BBR_RS12365 0.16 -1.2 525,461 + BBR_RS12365 0.17 +1.1 525,464 - BBR_RS12365 0.17 +1.1 525,475 + BBR_RS12365 0.19 -0.2 525,475 + BBR_RS12365 0.19 +1.6 525,476 - BBR_RS12365 0.19 -0.0 525,478 - BBR_RS12365 0.19 +1.0 525,480 + BBR_RS12365 0.20 -0.9 525,488 - BBR_RS12365 0.21 +2.1 525,488 - BBR_RS12365 0.21 -0.1 525,502 - BBR_RS12365 0.23 +0.5 525,528 - BBR_RS12365 0.27 -0.9 525,529 - BBR_RS12365 0.28 +2.8 525,533 - BBR_RS12365 0.28 -2.6 525,602 - BBR_RS12365 0.39 +0.9 525,612 - BBR_RS12365 0.41 +0.4 525,662 + BBR_RS12365 0.49 -0.4 525,667 + BBR_RS12365 0.50 +0.1 525,675 - BBR_RS12365 0.51 +0.1 525,675 - BBR_RS12365 0.51 +1.9 525,676 - BBR_RS12365 0.51 +0.7 525,716 + BBR_RS12365 0.58 -0.2 525,721 + BBR_RS12365 0.58 -1.1 525,735 + BBR_RS12365 0.61 +1.4 525,743 - BBR_RS12365 0.62 -0.2 525,844 - BBR_RS12365 0.78 -1.5 525,850 - BBR_RS12365 0.79 -0.4 525,854 - BBR_RS12365 0.80 +0.1 525,862 - BBR_RS12365 0.81 -0.5 525,890 - BBR_RS12365 0.86 -0.2 525,896 + BBR_RS12365 0.87 +0.1 525,905 + BBR_RS12365 0.88 +0.3 525,939 - -0.5 526,027 - -0.3 526,034 + +1.2 526,065 + +0.2 526,070 + +0.2 526,073 - -0.1 526,073 - -0.5 526,078 - +0.4 526,078 - -0.1 526,082 + +0.4 526,082 + +0.2 526,090 - -0.6 526,090 - +0.1 526,090 - +0.7 526,110 - -0.7 526,131 + -0.4 526,733 - BBR_RS12370 0.81 -0.7 526,891 - -0.2 526,896 - +0.2 526,898 + +0.8 526,916 + +0.9 527,083 - BBR_RS12375 0.40 +0.8 527,125 - BBR_RS12375 0.48 +1.2 527,212 - BBR_RS12375 0.65 +1.2 527,388 + +0.4 527,388 - +0.2 527,420 - -1.0 527,420 - +0.2 527,420 - -0.4 527,422 + +0.5 527,422 + +0.1 527,422 + -0.4 527,430 - -0.1 527,506 + -0.1 527,514 + -0.7 527,540 + BBR_RS12380 0.18 +0.7 527,558 - BBR_RS12380 0.24 -0.3 527,561 + BBR_RS12380 0.25 -0.5 527,574 - BBR_RS12380 0.30 -1.2 527,629 - BBR_RS12380 0.49 +0.0 527,636 + BBR_RS12380 0.52 +0.1 527,666 + BBR_RS12380 0.62 +0.4 527,671 + BBR_RS12380 0.64 -0.0 527,685 + BBR_RS12380 0.69 -1.6 527,698 - BBR_RS12380 0.74 +0.5 527,704 + BBR_RS12380 0.76 -0.7 527,704 + BBR_RS12380 0.76 +0.4 527,704 + BBR_RS12380 0.76 -1.0 527,712 + BBR_RS12380 0.79 -0.7 527,712 - BBR_RS12380 0.79 -0.7 527,734 - BBR_RS12380 0.87 +0.6 527,835 - -0.5
Or see this region's nucleotide sequence