Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12290 and BBR_RS12295 are separated by 22 nucleotides BBR_RS12295 and BBR_RS12300 are separated by 23 nucleotides BBR_RS12300 and BBR_RS12305 are separated by 219 nucleotides BBR_RS12305 and BBR_RS12310 are separated by 251 nucleotides
BBR_RS12290: BBR_RS12290 - ribosome maturation factor RimM, at 509,071 to 509,685
_RS12290
BBR_RS12295: BBR_RS12295 - RNA-binding protein, at 509,708 to 509,941
_RS12295
BBR_RS12300: BBR_RS12300 - 30S ribosomal protein S16, at 509,965 to 510,438
_RS12300
BBR_RS12305: BBR_RS12305 - endonuclease/exonuclease/phosphatase family protein, at 510,658 to 511,989
_RS12305
BBR_RS12310: BBR_RS12310 - signal recognition particle protein, at 512,241 to 513,896
_RS12310
Position (kb) 510
511
512 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 509.679 kb on - strand at 509.689 kb on - strand at 509.689 kb on - strand at 509.707 kb on - strand at 509.729 kb on - strand at 509.787 kb on - strand, within BBR_RS12295 at 509.936 kb on - strand at 509.940 kb on - strand at 509.952 kb on - strand at 509.982 kb on - strand at 510.049 kb on - strand, within BBR_RS12300 at 510.049 kb on - strand, within BBR_RS12300 at 510.061 kb on - strand, within BBR_RS12300 at 510.154 kb on - strand, within BBR_RS12300 at 510.185 kb on - strand, within BBR_RS12300 at 510.567 kb on + strand at 510.580 kb on + strand at 510.580 kb on + strand at 510.588 kb on - strand at 510.588 kb on - strand at 510.588 kb on - strand at 510.593 kb on - strand at 510.593 kb on - strand at 510.602 kb on + strand at 510.603 kb on - strand at 510.612 kb on - strand at 510.645 kb on + strand at 510.692 kb on - strand at 510.698 kb on - strand at 510.716 kb on + strand at 510.724 kb on - strand at 510.812 kb on - strand, within BBR_RS12305 at 510.813 kb on - strand, within BBR_RS12305 at 510.858 kb on + strand, within BBR_RS12305 at 511.033 kb on - strand, within BBR_RS12305 at 511.044 kb on - strand, within BBR_RS12305 at 511.050 kb on + strand, within BBR_RS12305 at 511.080 kb on + strand, within BBR_RS12305 at 511.080 kb on + strand, within BBR_RS12305 at 511.080 kb on + strand, within BBR_RS12305 at 511.122 kb on - strand, within BBR_RS12305 at 511.158 kb on - strand, within BBR_RS12305 at 511.222 kb on - strand, within BBR_RS12305 at 511.224 kb on - strand, within BBR_RS12305 at 511.242 kb on - strand, within BBR_RS12305 at 511.254 kb on - strand, within BBR_RS12305 at 511.300 kb on + strand, within BBR_RS12305 at 511.410 kb on + strand, within BBR_RS12305 at 511.436 kb on + strand, within BBR_RS12305 at 511.507 kb on - strand, within BBR_RS12305 at 511.547 kb on + strand, within BBR_RS12305 at 511.571 kb on - strand, within BBR_RS12305 at 511.573 kb on - strand, within BBR_RS12305 at 511.622 kb on + strand, within BBR_RS12305 at 511.622 kb on + strand, within BBR_RS12305 at 511.635 kb on - strand, within BBR_RS12305 at 511.644 kb on - strand, within BBR_RS12305 at 511.701 kb on + strand, within BBR_RS12305 at 511.701 kb on + strand, within BBR_RS12305 at 511.718 kb on + strand, within BBR_RS12305 at 511.744 kb on - strand, within BBR_RS12305 at 511.751 kb on + strand, within BBR_RS12305 at 511.758 kb on + strand, within BBR_RS12305 at 511.775 kb on + strand, within BBR_RS12305 at 511.777 kb on + strand, within BBR_RS12305 at 511.793 kb on + strand, within BBR_RS12305 at 511.852 kb on + strand, within BBR_RS12305 at 511.860 kb on - strand at 511.865 kb on + strand at 511.875 kb on - strand at 511.886 kb on + strand at 511.925 kb on - strand at 511.941 kb on - strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.951 kb on - strand at 511.951 kb on - strand at 511.965 kb on + strand at 511.970 kb on + strand at 511.970 kb on + strand at 511.978 kb on - strand at 512.039 kb on - strand at 512.051 kb on + strand at 512.057 kb on - strand at 512.062 kb on - strand at 512.062 kb on - strand at 512.063 kb on - strand at 512.087 kb on - strand at 512.112 kb on - strand at 512.159 kb on - strand at 512.188 kb on - strand at 512.235 kb on + strand at 512.258 kb on - strand at 512.285 kb on - strand at 512.288 kb on + strand at 512.296 kb on - strand at 512.321 kb on + strand at 512.329 kb on - strand at 512.329 kb on - strand at 512.329 kb on - strand at 512.341 kb on + strand at 512.422 kb on + strand, within BBR_RS12310 at 512.435 kb on - strand, within BBR_RS12310 at 512.559 kb on - strand, within BBR_RS12310
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 509,679 - +0.3 509,689 - +0.1 509,689 - +0.7 509,707 - -0.3 509,729 - +1.2 509,787 - BBR_RS12295 0.34 -0.8 509,936 - -0.0 509,940 - -0.4 509,952 - -0.9 509,982 - +0.5 510,049 - BBR_RS12300 0.18 -0.9 510,049 - BBR_RS12300 0.18 -0.3 510,061 - BBR_RS12300 0.20 -0.8 510,154 - BBR_RS12300 0.40 -0.4 510,185 - BBR_RS12300 0.46 -0.8 510,567 + -0.7 510,580 + +0.5 510,580 + +0.6 510,588 - -2.2 510,588 - -0.2 510,588 - +0.2 510,593 - -0.4 510,593 - +0.0 510,602 + +0.1 510,603 - +0.1 510,612 - +0.6 510,645 + +0.4 510,692 - -0.2 510,698 - -0.8 510,716 + -0.6 510,724 - +1.7 510,812 - BBR_RS12305 0.12 +0.7 510,813 - BBR_RS12305 0.12 -0.6 510,858 + BBR_RS12305 0.15 -0.7 511,033 - BBR_RS12305 0.28 +0.7 511,044 - BBR_RS12305 0.29 -0.9 511,050 + BBR_RS12305 0.29 +0.1 511,080 + BBR_RS12305 0.32 +0.1 511,080 + BBR_RS12305 0.32 -1.1 511,080 + BBR_RS12305 0.32 -0.4 511,122 - BBR_RS12305 0.35 -0.6 511,158 - BBR_RS12305 0.38 +0.1 511,222 - BBR_RS12305 0.42 -0.6 511,224 - BBR_RS12305 0.42 -0.5 511,242 - BBR_RS12305 0.44 -0.3 511,254 - BBR_RS12305 0.45 -1.2 511,300 + BBR_RS12305 0.48 +0.5 511,410 + BBR_RS12305 0.56 -0.8 511,436 + BBR_RS12305 0.58 -0.3 511,507 - BBR_RS12305 0.64 +0.4 511,547 + BBR_RS12305 0.67 -0.7 511,571 - BBR_RS12305 0.69 -0.9 511,573 - BBR_RS12305 0.69 +0.0 511,622 + BBR_RS12305 0.72 -0.6 511,622 + BBR_RS12305 0.72 +0.6 511,635 - BBR_RS12305 0.73 +1.0 511,644 - BBR_RS12305 0.74 -0.9 511,701 + BBR_RS12305 0.78 -0.8 511,701 + BBR_RS12305 0.78 -0.8 511,718 + BBR_RS12305 0.80 -2.9 511,744 - BBR_RS12305 0.82 +0.3 511,751 + BBR_RS12305 0.82 -0.0 511,758 + BBR_RS12305 0.83 -0.6 511,775 + BBR_RS12305 0.84 -0.8 511,777 + BBR_RS12305 0.84 -2.3 511,793 + BBR_RS12305 0.85 -2.4 511,852 + BBR_RS12305 0.90 +0.1 511,860 - -0.8 511,865 + -1.5 511,875 - -0.2 511,886 + -1.7 511,925 - -1.3 511,941 - -0.1 511,943 + -1.7 511,943 + -0.6 511,943 + -3.1 511,943 + -1.5 511,943 + -1.0 511,943 + -0.3 511,943 + -0.6 511,943 + -1.3 511,951 - -0.7 511,951 - +0.1 511,965 + +0.4 511,970 + -0.4 511,970 + -1.4 511,978 - -1.0 512,039 - -0.7 512,051 + +0.7 512,057 - +0.3 512,062 - -0.1 512,062 - -0.5 512,063 - -1.3 512,087 - -0.2 512,112 - +0.1 512,159 - +0.8 512,188 - +0.8 512,235 + +0.0 512,258 - +0.1 512,285 - -0.5 512,288 + +0.9 512,296 - +0.9 512,321 + +0.5 512,329 - +0.5 512,329 - -0.2 512,329 - +0.8 512,341 + +0.7 512,422 + BBR_RS12310 0.11 +0.6 512,435 - BBR_RS12310 0.12 +0.6 512,559 - BBR_RS12310 0.19 -1.2
Or see this region's nucleotide sequence