Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS12295

Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
508,825	-		BBR_RS12285	0.84	+0.5
508,896	+				+0.4
508,915	-				-0.6
508,924	-				+0.4
508,933	+				+0.1
508,941	-				-0.4
508,993	+				+0.4
509,004	+				+0.5
509,005	+				+0.1
509,043	+				-1.0
509,043	+				-1.2
509,062	+				-0.8
509,063	+				-0.7
509,070	-				-0.4
509,084	-				-0.1
509,090	-				-0.1
509,641	-				+0.4
509,641	-				+0.5
509,641	-				-0.2
509,679	-				+0.3
509,689	-				+0.1
509,689	-				+0.7
509,707	-				-0.3
509,729	-				+1.2
509,787	-		BBR_RS12295	0.34	-0.8
509,936	-				-0.0
509,940	-				-0.4
509,952	-				-0.9
509,982	-				+0.5
510,049	-		BBR_RS12300	0.18	-0.9
510,049	-		BBR_RS12300	0.18	-0.3
510,061	-		BBR_RS12300	0.20	-0.8
510,154	-		BBR_RS12300	0.40	-0.4
510,185	-		BBR_RS12300	0.46	-0.8
510,567	+				-0.7
510,580	+				+0.5
510,580	+				+0.6
510,588	-				-2.2
510,588	-				-0.2
510,588	-				+0.2
510,593	-				-0.4
510,593	-				+0.0
510,602	+				+0.1
510,603	-				+0.1
510,612	-				+0.6
510,645	+				+0.4
510,692	-				-0.2
510,698	-				-0.8
510,716	+				-0.6
510,724	-				+1.7
510,812	-		BBR_RS12305	0.12	+0.7
510,813	-		BBR_RS12305	0.12	-0.6
510,858	+		BBR_RS12305	0.15	-0.7