Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20675 and BBR_RS20680 overlap by 16 nucleotides BBR_RS20680 and BBR_RS12235 are separated by 86 nucleotides BBR_RS12235 and BBR_RS12240 are separated by 129 nucleotides BBR_RS12240 and BBR_RS20685 overlap by 40 nucleotides BBR_RS20685 and BBR_RS12245 are separated by 40 nucleotides
BBR_RS20675: BBR_RS20675 - hypothetical protein, at 493,926 to 494,420
_RS20675
BBR_RS20680: BBR_RS20680 - hypothetical protein, at 494,405 to 494,641
_RS20680
BBR_RS12235: BBR_RS12235 - hypothetical protein, at 494,728 to 495,414
_RS12235
BBR_RS12240: BBR_RS12240 - 30S ribosomal protein S15, at 495,544 to 495,813
_RS12240
BBR_RS20685: BBR_RS20685 - hypothetical protein, at 495,774 to 496,049
_RS20685
BBR_RS12245: BBR_RS12245 - polyribonucleotide nucleotidyltransferase, at 496,090 to 498,801
_RS12245
Position (kb) 494
495
496 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 493.742 kb on + strand at 493.742 kb on + strand at 493.763 kb on + strand at 493.771 kb on - strand at 493.773 kb on + strand at 493.773 kb on + strand at 493.814 kb on + strand at 493.820 kb on - strand at 493.825 kb on - strand at 493.825 kb on - strand at 493.850 kb on + strand at 493.876 kb on + strand at 493.884 kb on - strand at 493.884 kb on - strand at 493.901 kb on + strand at 493.910 kb on + strand at 493.910 kb on + strand at 493.910 kb on + strand at 493.911 kb on - strand at 493.918 kb on - strand at 493.920 kb on + strand at 493.920 kb on - strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.992 kb on + strand, within BBR_RS20675 at 494.001 kb on + strand, within BBR_RS20675 at 494.001 kb on + strand, within BBR_RS20675 at 494.021 kb on - strand, within BBR_RS20675 at 494.037 kb on - strand, within BBR_RS20675 at 494.114 kb on + strand, within BBR_RS20675 at 494.127 kb on - strand, within BBR_RS20675 at 494.129 kb on + strand, within BBR_RS20675 at 494.163 kb on - strand, within BBR_RS20675 at 494.163 kb on - strand, within BBR_RS20675 at 494.224 kb on - strand, within BBR_RS20675 at 494.248 kb on + strand, within BBR_RS20675 at 494.266 kb on + strand, within BBR_RS20675 at 494.390 kb on + strand at 494.390 kb on + strand at 494.390 kb on + strand at 494.390 kb on + strand at 494.395 kb on + strand at 494.395 kb on + strand at 494.423 kb on + strand at 494.440 kb on - strand, within BBR_RS20680 at 494.440 kb on - strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.478 kb on - strand, within BBR_RS20680 at 494.482 kb on + strand, within BBR_RS20680 at 494.491 kb on - strand, within BBR_RS20680 at 494.588 kb on + strand, within BBR_RS20680 at 494.604 kb on + strand, within BBR_RS20680 at 494.618 kb on - strand at 494.626 kb on + strand at 494.626 kb on + strand at 494.628 kb on - strand at 494.634 kb on - strand at 494.636 kb on - strand at 494.636 kb on - strand at 494.657 kb on - strand at 494.672 kb on + strand at 494.676 kb on + strand at 494.684 kb on - strand at 494.716 kb on + strand at 494.726 kb on + strand at 494.731 kb on + strand at 494.733 kb on + strand at 494.755 kb on + strand at 494.787 kb on + strand at 494.787 kb on + strand at 494.871 kb on + strand, within BBR_RS12235 at 495.834 kb on + strand, within BBR_RS20685 at 495.840 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.892 kb on + strand, within BBR_RS20685 at 495.893 kb on + strand, within BBR_RS20685 at 495.893 kb on + strand, within BBR_RS20685 at 495.911 kb on + strand, within BBR_RS20685 at 495.927 kb on - strand, within BBR_RS20685 at 495.941 kb on - strand, within BBR_RS20685 at 495.988 kb on + strand, within BBR_RS20685 at 496.029 kb on + strand at 496.031 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 493,742 + +0.3 493,742 + -0.2 493,763 + +0.0 493,771 - +1.0 493,773 + +0.4 493,773 + -0.6 493,814 + +0.0 493,820 - -1.1 493,825 - -0.2 493,825 - -0.6 493,850 + -0.7 493,876 + -0.3 493,884 - -0.8 493,884 - +0.3 493,901 + +0.2 493,910 + -0.1 493,910 + -0.7 493,910 + -0.7 493,911 - -0.5 493,918 - -0.1 493,920 + -0.4 493,920 - +0.5 493,959 - +0.4 493,959 - +0.1 493,992 + BBR_RS20675 0.13 +0.0 494,001 + BBR_RS20675 0.15 +0.2 494,001 + BBR_RS20675 0.15 +0.3 494,021 - BBR_RS20675 0.19 -0.6 494,037 - BBR_RS20675 0.22 +0.9 494,114 + BBR_RS20675 0.38 -0.1 494,127 - BBR_RS20675 0.41 -0.4 494,129 + BBR_RS20675 0.41 +0.9 494,163 - BBR_RS20675 0.48 -1.3 494,163 - BBR_RS20675 0.48 -0.4 494,224 - BBR_RS20675 0.60 +0.2 494,248 + BBR_RS20675 0.65 -0.1 494,266 + BBR_RS20675 0.69 -0.5 494,390 + +0.0 494,390 + +0.4 494,390 + -0.8 494,390 + +0.1 494,395 + -0.6 494,395 + +0.2 494,423 + +0.2 494,440 - BBR_RS20680 0.15 +0.5 494,440 - BBR_RS20680 0.15 +0.1 494,470 + BBR_RS20680 0.27 +0.6 494,470 + BBR_RS20680 0.27 -0.1 494,470 + BBR_RS20680 0.27 +0.5 494,478 - BBR_RS20680 0.31 -0.2 494,482 + BBR_RS20680 0.32 +0.1 494,491 - BBR_RS20680 0.36 -0.1 494,588 + BBR_RS20680 0.77 +0.4 494,604 + BBR_RS20680 0.84 +0.3 494,618 - +1.1 494,626 + -0.1 494,626 + -0.4 494,628 - +0.1 494,634 - +0.5 494,636 - -0.1 494,636 - +0.9 494,657 - +0.2 494,672 + -1.3 494,676 + +0.5 494,684 - -1.2 494,716 + -1.9 494,726 + -2.8 494,731 + -0.2 494,733 + +0.3 494,755 + -1.6 494,787 + -0.1 494,787 + -1.0 494,871 + BBR_RS12235 0.21 -3.8 495,834 + BBR_RS20685 0.22 -0.4 495,840 + BBR_RS20685 0.24 +0.2 495,845 + BBR_RS20685 0.26 +0.2 495,845 + BBR_RS20685 0.26 +0.8 495,845 + BBR_RS20685 0.26 -0.3 495,892 + BBR_RS20685 0.43 +0.2 495,893 + BBR_RS20685 0.43 +0.6 495,893 + BBR_RS20685 0.43 +0.1 495,911 + BBR_RS20685 0.50 +1.2 495,927 - BBR_RS20685 0.55 +0.8 495,941 - BBR_RS20685 0.61 +0.8 495,988 + BBR_RS20685 0.78 -0.2 496,029 + +0.1 496,031 + +0.4
Or see this region's nucleotide sequence