Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12040 and BBR_RS12045 are separated by 101 nucleotides BBR_RS12045 and BBR_RS12050 overlap by 1 nucleotides
BBR_RS12040: BBR_RS12040 - peptidyl-prolyl cis-trans isomerase, at 450,815 to 451,768
_RS12040
BBR_RS12045: BBR_RS12045 - hypothetical protein, at 451,870 to 453,108
_RS12045
BBR_RS12050: BBR_RS12050 - hypothetical protein, at 453,108 to 454,112
_RS12050
Position (kb)
451
452
453
454 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 450.888 kb on + strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.952 kb on - strand, within BBR_RS12040 at 450.963 kb on - strand, within BBR_RS12040 at 450.975 kb on - strand, within BBR_RS12040 at 450.975 kb on - strand, within BBR_RS12040 at 450.995 kb on - strand, within BBR_RS12040 at 451.001 kb on + strand, within BBR_RS12040 at 451.001 kb on + strand, within BBR_RS12040 at 451.016 kb on + strand, within BBR_RS12040 at 451.019 kb on - strand, within BBR_RS12040 at 451.019 kb on - strand, within BBR_RS12040 at 451.024 kb on + strand, within BBR_RS12040 at 451.026 kb on + strand, within BBR_RS12040 at 451.026 kb on + strand, within BBR_RS12040 at 451.057 kb on + strand, within BBR_RS12040 at 451.102 kb on - strand, within BBR_RS12040 at 451.113 kb on + strand, within BBR_RS12040 at 451.115 kb on + strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.158 kb on - strand, within BBR_RS12040 at 451.158 kb on - strand, within BBR_RS12040 at 451.158 kb on - strand, within BBR_RS12040 at 451.160 kb on + strand, within BBR_RS12040 at 451.160 kb on + strand, within BBR_RS12040 at 451.160 kb on + strand, within BBR_RS12040 at 451.176 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.226 kb on + strand, within BBR_RS12040 at 451.226 kb on + strand, within BBR_RS12040 at 451.231 kb on - strand, within BBR_RS12040 at 451.231 kb on - strand, within BBR_RS12040 at 451.232 kb on - strand, within BBR_RS12040 at 451.232 kb on - strand, within BBR_RS12040 at 451.234 kb on + strand, within BBR_RS12040 at 451.234 kb on + strand, within BBR_RS12040 at 451.258 kb on + strand, within BBR_RS12040 at 451.265 kb on - strand, within BBR_RS12040 at 451.349 kb on - strand, within BBR_RS12040 at 451.364 kb on - strand, within BBR_RS12040 at 451.374 kb on - strand, within BBR_RS12040 at 451.377 kb on + strand, within BBR_RS12040 at 451.385 kb on - strand, within BBR_RS12040 at 451.455 kb on - strand, within BBR_RS12040 at 451.532 kb on - strand, within BBR_RS12040 at 451.662 kb on - strand, within BBR_RS12040 at 451.670 kb on - strand, within BBR_RS12040 at 451.748 kb on - strand at 451.750 kb on + strand at 451.770 kb on + strand at 451.770 kb on + strand at 451.778 kb on - strand at 451.821 kb on - strand at 451.843 kb on + strand at 451.853 kb on - strand at 451.896 kb on + strand at 451.961 kb on + strand at 451.971 kb on + strand at 452.010 kb on + strand, within BBR_RS12045 at 452.059 kb on + strand, within BBR_RS12045 at 452.071 kb on + strand, within BBR_RS12045 at 452.857 kb on + strand, within BBR_RS12045 at 452.932 kb on + strand, within BBR_RS12045 at 452.932 kb on + strand, within BBR_RS12045 at 452.948 kb on + strand, within BBR_RS12045 at 452.948 kb on + strand, within BBR_RS12045 at 452.950 kb on + strand, within BBR_RS12045 at 452.950 kb on + strand, within BBR_RS12045 at 452.953 kb on + strand, within BBR_RS12045 at 452.958 kb on + strand, within BBR_RS12045 at 452.966 kb on - strand, within BBR_RS12045 at 453.021 kb on + strand at 453.021 kb on + strand at 453.021 kb on + strand at 453.051 kb on + strand at 453.051 kb on - strand at 453.051 kb on - strand at 453.066 kb on - strand at 453.093 kb on + strand at 453.097 kb on + strand at 453.107 kb on + strand at 453.110 kb on - strand at 454.097 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 450,888 + -0.9 450,901 - -0.4 450,901 - -0.8 450,901 - -0.5 450,901 - -0.3 450,901 - -0.3 450,901 - -0.2 450,952 - BBR_RS12040 0.14 -0.3 450,963 - BBR_RS12040 0.16 +0.4 450,975 - BBR_RS12040 0.17 +0.2 450,975 - BBR_RS12040 0.17 -0.6 450,995 - BBR_RS12040 0.19 +0.5 451,001 + BBR_RS12040 0.19 -0.7 451,001 + BBR_RS12040 0.19 -1.1 451,016 + BBR_RS12040 0.21 -0.7 451,019 - BBR_RS12040 0.21 +0.0 451,019 - BBR_RS12040 0.21 +0.4 451,024 + BBR_RS12040 0.22 -0.5 451,026 + BBR_RS12040 0.22 -1.4 451,026 + BBR_RS12040 0.22 +0.4 451,057 + BBR_RS12040 0.25 -0.2 451,102 - BBR_RS12040 0.30 +0.4 451,113 + BBR_RS12040 0.31 -0.6 451,115 + BBR_RS12040 0.31 -1.3 451,121 - BBR_RS12040 0.32 +0.8 451,121 - BBR_RS12040 0.32 +0.9 451,121 - BBR_RS12040 0.32 -0.0 451,121 - BBR_RS12040 0.32 -0.1 451,158 - BBR_RS12040 0.36 +0.1 451,158 - BBR_RS12040 0.36 -0.5 451,158 - BBR_RS12040 0.36 -1.5 451,160 + BBR_RS12040 0.36 +0.9 451,160 + BBR_RS12040 0.36 -0.9 451,160 + BBR_RS12040 0.36 -0.2 451,176 + BBR_RS12040 0.38 +0.3 451,224 + BBR_RS12040 0.43 +0.7 451,224 + BBR_RS12040 0.43 -0.6 451,224 + BBR_RS12040 0.43 -0.2 451,224 + BBR_RS12040 0.43 -0.0 451,226 + BBR_RS12040 0.43 +0.2 451,226 + BBR_RS12040 0.43 -0.3 451,231 - BBR_RS12040 0.44 +0.2 451,231 - BBR_RS12040 0.44 +0.3 451,232 - BBR_RS12040 0.44 +0.2 451,232 - BBR_RS12040 0.44 +0.5 451,234 + BBR_RS12040 0.44 -1.4 451,234 + BBR_RS12040 0.44 -0.9 451,258 + BBR_RS12040 0.46 -0.3 451,265 - BBR_RS12040 0.47 -0.6 451,349 - BBR_RS12040 0.56 +0.8 451,364 - BBR_RS12040 0.58 +0.8 451,374 - BBR_RS12040 0.59 -0.2 451,377 + BBR_RS12040 0.59 +0.4 451,385 - BBR_RS12040 0.60 +2.4 451,455 - BBR_RS12040 0.67 +0.1 451,532 - BBR_RS12040 0.75 -1.1 451,662 - BBR_RS12040 0.89 +1.4 451,670 - BBR_RS12040 0.90 +0.8 451,748 - -0.2 451,750 + +1.4 451,770 + +0.4 451,770 + +0.3 451,778 - +0.0 451,821 - +1.1 451,843 + +0.0 451,853 - +0.1 451,896 + -0.6 451,961 + -0.2 451,971 + -0.5 452,010 + BBR_RS12045 0.11 -1.6 452,059 + BBR_RS12045 0.15 -2.2 452,071 + BBR_RS12045 0.16 -0.2 452,857 + BBR_RS12045 0.80 -1.9 452,932 + BBR_RS12045 0.86 -1.8 452,932 + BBR_RS12045 0.86 -1.6 452,948 + BBR_RS12045 0.87 -2.0 452,948 + BBR_RS12045 0.87 -0.6 452,950 + BBR_RS12045 0.87 -3.1 452,950 + BBR_RS12045 0.87 -1.2 452,953 + BBR_RS12045 0.87 +0.6 452,958 + BBR_RS12045 0.88 -1.7 452,966 - BBR_RS12045 0.88 +0.1 453,021 + -1.2 453,021 + -1.6 453,021 + -0.4 453,051 + -0.4 453,051 - -1.5 453,051 - -1.8 453,066 - -1.3 453,093 + -0.2 453,097 + -0.2 453,107 + -0.2 453,110 - +0.2 454,097 + -0.1
Or see this region's nucleotide sequence