Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11795 and BBR_RS11800 are separated by 175 nucleotides BBR_RS11800 and BBR_RS11805 are separated by 59 nucleotides BBR_RS11805 and BBR_RS11810 are separated by 233 nucleotides BBR_RS11810 and BBR_RS11815 are separated by 85 nucleotides
BBR_RS11795: BBR_RS11795 - DNA-binding response regulator, at 405,623 to 406,393
_RS11795
BBR_RS11800: BBR_RS11800 - hypothetical protein, at 406,569 to 407,273
_RS11800
BBR_RS11805: BBR_RS11805 - hypothetical protein, at 407,333 to 407,911
_RS11805
BBR_RS11810: BBR_RS11810 - inorganic pyrophosphatase, at 408,145 to 408,639
_RS11810
BBR_RS11815: BBR_RS11815 - DUF3416 domain-containing protein, at 408,725 to 410,953
_RS11815
Position (kb) 407
408 Strain fitness (log2 ratio) -2
-1
0
1
2 at 406.411 kb on + strand at 406.411 kb on + strand at 406.419 kb on - strand at 406.420 kb on + strand at 406.424 kb on + strand at 406.428 kb on - strand at 406.428 kb on - strand at 406.467 kb on - strand at 406.502 kb on - strand at 406.517 kb on - strand at 406.520 kb on - strand at 406.520 kb on - strand at 406.520 kb on - strand at 407.261 kb on - strand at 407.271 kb on - strand at 407.281 kb on - strand at 407.281 kb on - strand at 407.305 kb on - strand at 407.305 kb on - strand at 407.323 kb on + strand at 407.326 kb on + strand at 407.331 kb on - strand at 407.331 kb on - strand at 407.360 kb on + strand at 407.362 kb on + strand at 407.368 kb on + strand at 407.408 kb on + strand, within BBR_RS11805 at 407.448 kb on + strand, within BBR_RS11805 at 407.468 kb on + strand, within BBR_RS11805 at 407.485 kb on + strand, within BBR_RS11805 at 407.497 kb on - strand, within BBR_RS11805 at 407.528 kb on - strand, within BBR_RS11805 at 407.553 kb on - strand, within BBR_RS11805 at 407.721 kb on - strand, within BBR_RS11805 at 407.736 kb on + strand, within BBR_RS11805 at 407.781 kb on - strand, within BBR_RS11805 at 407.790 kb on + strand, within BBR_RS11805 at 407.809 kb on - strand, within BBR_RS11805 at 407.809 kb on - strand, within BBR_RS11805 at 407.826 kb on + strand, within BBR_RS11805 at 407.826 kb on + strand, within BBR_RS11805 at 407.832 kb on - strand, within BBR_RS11805 at 407.833 kb on - strand, within BBR_RS11805 at 407.848 kb on - strand, within BBR_RS11805 at 407.879 kb on + strand at 407.882 kb on + strand at 407.887 kb on - strand at 407.889 kb on + strand at 407.896 kb on - strand at 407.907 kb on + strand at 407.939 kb on + strand at 407.939 kb on + strand at 407.954 kb on - strand at 407.964 kb on - strand at 407.970 kb on - strand at 407.977 kb on + strand at 408.000 kb on + strand at 408.008 kb on + strand at 408.008 kb on - strand at 408.008 kb on - strand at 408.008 kb on - strand at 408.010 kb on - strand at 408.016 kb on - strand at 408.044 kb on - strand at 408.052 kb on + strand at 408.070 kb on + strand at 408.072 kb on + strand at 408.080 kb on - strand at 408.140 kb on - strand at 408.670 kb on - strand at 408.670 kb on - strand at 408.670 kb on - strand at 408.672 kb on - strand at 408.673 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.678 kb on + strand at 408.678 kb on + strand at 408.680 kb on + strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.686 kb on - strand at 408.686 kb on - strand at 408.686 kb on - strand at 408.693 kb on + strand at 408.699 kb on + strand at 408.703 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.716 kb on + strand at 408.790 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 406,411 + -0.9 406,411 + -0.7 406,419 - +0.5 406,420 + -1.1 406,424 + -1.3 406,428 - +0.0 406,428 - -0.7 406,467 - -0.0 406,502 - -0.2 406,517 - -0.3 406,520 - -0.0 406,520 - -0.3 406,520 - -1.2 407,261 - -0.0 407,271 - -1.0 407,281 - -0.9 407,281 - -1.0 407,305 - -0.5 407,305 - -0.6 407,323 + +0.1 407,326 + -0.3 407,331 - -0.4 407,331 - +0.4 407,360 + -0.8 407,362 + -0.4 407,368 + -1.1 407,408 + BBR_RS11805 0.13 -0.8 407,448 + BBR_RS11805 0.20 -0.5 407,468 + BBR_RS11805 0.23 -0.3 407,485 + BBR_RS11805 0.26 -0.2 407,497 - BBR_RS11805 0.28 -1.0 407,528 - BBR_RS11805 0.34 -1.3 407,553 - BBR_RS11805 0.38 -0.7 407,721 - BBR_RS11805 0.67 -0.4 407,736 + BBR_RS11805 0.70 -0.8 407,781 - BBR_RS11805 0.77 +0.3 407,790 + BBR_RS11805 0.79 +0.3 407,809 - BBR_RS11805 0.82 -1.4 407,809 - BBR_RS11805 0.82 -1.3 407,826 + BBR_RS11805 0.85 +0.8 407,826 + BBR_RS11805 0.85 -0.5 407,832 - BBR_RS11805 0.86 -1.1 407,833 - BBR_RS11805 0.86 -1.0 407,848 - BBR_RS11805 0.89 -0.6 407,879 + -1.3 407,882 + -0.4 407,887 - -0.2 407,889 + -1.5 407,896 - -0.3 407,907 + +0.7 407,939 + -2.0 407,939 + -1.6 407,954 - -1.9 407,964 - -1.0 407,970 - -1.2 407,977 + -0.5 408,000 + +0.1 408,008 + +0.2 408,008 - -0.8 408,008 - -0.0 408,008 - -0.5 408,010 - -0.1 408,016 - +0.6 408,044 - -0.4 408,052 + -1.3 408,070 + +0.2 408,072 + -0.5 408,080 - -0.5 408,140 - +0.1 408,670 - -0.1 408,670 - -0.7 408,670 - +1.0 408,672 - +0.6 408,673 + +1.0 408,676 + +0.1 408,676 + -0.4 408,676 + -0.5 408,676 + -0.7 408,676 + +0.2 408,676 + -0.6 408,676 + +0.2 408,678 + -0.6 408,678 + +0.3 408,680 + -0.7 408,684 - -0.5 408,684 - -0.2 408,684 - -1.3 408,684 - -1.1 408,684 - +0.1 408,684 - +0.4 408,684 - -0.6 408,684 - -0.1 408,686 - -0.8 408,686 - +0.4 408,686 - +2.4 408,693 + -0.5 408,699 + +0.4 408,703 - -0.7 408,707 - -0.5 408,707 - -0.3 408,707 - +0.6 408,707 - -0.1 408,707 - +0.7 408,707 - -0.4 408,707 - -0.5 408,707 - -1.2 408,716 + +0.0 408,790 - +0.8
Or see this region's nucleotide sequence