Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11760 and BBR_RS11765 overlap by 2 nucleotides BBR_RS11765 and BBR_RS11770 are separated by 77 nucleotides BBR_RS11770 and BBR_RS11775 are separated by 146 nucleotides
BBR_RS11760: BBR_RS11760 - integrase, at 396,195 to 397,658
_RS11760
BBR_RS11765: BBR_RS11765 - IS3 family transposase, at 397,657 to 397,947
_RS11765
BBR_RS11770: BBR_RS11770 - chorismate mutase, at 398,025 to 398,414
_RS11770
BBR_RS11775: BBR_RS11775 - hypothetical protein, at 398,561 to 400,546
_RS11775
Position (kb)
398
399 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 397.025 kb on - strand, within BBR_RS11760 at 397.039 kb on + strand, within BBR_RS11760 at 397.087 kb on + strand, within BBR_RS11760 at 397.091 kb on + strand, within BBR_RS11760 at 397.124 kb on + strand, within BBR_RS11760 at 397.174 kb on + strand, within BBR_RS11760 at 397.182 kb on + strand, within BBR_RS11760 at 397.182 kb on + strand, within BBR_RS11760 at 397.182 kb on + strand, within BBR_RS11760 at 397.182 kb on + strand, within BBR_RS11760 at 397.190 kb on - strand, within BBR_RS11760 at 397.202 kb on + strand, within BBR_RS11760 at 397.205 kb on - strand, within BBR_RS11760 at 397.223 kb on - strand, within BBR_RS11760 at 397.225 kb on + strand, within BBR_RS11760 at 397.225 kb on + strand, within BBR_RS11760 at 397.255 kb on - strand, within BBR_RS11760 at 397.255 kb on - strand, within BBR_RS11760 at 397.272 kb on + strand, within BBR_RS11760 at 397.292 kb on + strand, within BBR_RS11760 at 397.297 kb on + strand, within BBR_RS11760 at 397.297 kb on + strand, within BBR_RS11760 at 397.305 kb on - strand, within BBR_RS11760 at 397.342 kb on + strand, within BBR_RS11760 at 397.346 kb on - strand, within BBR_RS11760 at 397.405 kb on + strand, within BBR_RS11760 at 397.405 kb on - strand, within BBR_RS11760 at 397.415 kb on + strand, within BBR_RS11760 at 397.433 kb on - strand, within BBR_RS11760 at 397.445 kb on + strand, within BBR_RS11760 at 397.445 kb on + strand, within BBR_RS11760 at 397.453 kb on + strand, within BBR_RS11760 at 397.453 kb on - strand, within BBR_RS11760 at 397.461 kb on - strand, within BBR_RS11760 at 397.469 kb on + strand, within BBR_RS11760 at 397.477 kb on - strand, within BBR_RS11760 at 397.482 kb on - strand, within BBR_RS11760 at 397.482 kb on - strand, within BBR_RS11760 at 397.482 kb on - strand, within BBR_RS11760 at 397.511 kb on - strand, within BBR_RS11760 at 397.516 kb on - strand at 397.520 kb on - strand at 397.522 kb on + strand at 397.530 kb on - strand at 397.530 kb on - strand at 397.556 kb on - strand at 397.556 kb on - strand at 397.556 kb on - strand at 397.564 kb on - strand at 397.571 kb on + strand at 397.592 kb on + strand at 397.592 kb on + strand at 397.602 kb on + strand at 397.602 kb on - strand at 397.626 kb on + strand at 397.636 kb on + strand at 397.636 kb on + strand at 397.638 kb on + strand at 397.644 kb on - strand at 397.644 kb on - strand at 397.651 kb on + strand at 397.651 kb on + strand at 397.659 kb on - strand at 397.670 kb on + strand at 397.684 kb on + strand at 397.692 kb on - strand, within BBR_RS11765 at 397.702 kb on - strand, within BBR_RS11765 at 397.744 kb on + strand, within BBR_RS11765 at 397.750 kb on + strand, within BBR_RS11765 at 397.758 kb on - strand, within BBR_RS11765 at 397.759 kb on - strand, within BBR_RS11765 at 397.761 kb on + strand, within BBR_RS11765 at 397.776 kb on + strand, within BBR_RS11765 at 397.800 kb on + strand, within BBR_RS11765 at 397.800 kb on + strand, within BBR_RS11765 at 397.808 kb on + strand, within BBR_RS11765 at 397.836 kb on + strand, within BBR_RS11765 at 397.836 kb on + strand, within BBR_RS11765 at 397.836 kb on + strand, within BBR_RS11765 at 397.844 kb on - strand, within BBR_RS11765 at 397.844 kb on - strand, within BBR_RS11765 at 397.851 kb on - strand, within BBR_RS11765 at 397.854 kb on + strand, within BBR_RS11765 at 397.856 kb on + strand, within BBR_RS11765 at 397.867 kb on + strand, within BBR_RS11765 at 397.869 kb on - strand, within BBR_RS11765 at 397.883 kb on - strand, within BBR_RS11765 at 397.890 kb on + strand, within BBR_RS11765 at 397.910 kb on + strand, within BBR_RS11765 at 397.918 kb on - strand, within BBR_RS11765 at 397.942 kb on + strand at 397.942 kb on + strand at 397.942 kb on + strand at 397.950 kb on - strand at 397.951 kb on + strand at 397.968 kb on - strand at 397.998 kb on + strand at 398.022 kb on + strand at 398.122 kb on + strand, within BBR_RS11770 at 398.156 kb on + strand, within BBR_RS11770 at 398.171 kb on - strand, within BBR_RS11770 at 398.179 kb on - strand, within BBR_RS11770 at 398.207 kb on + strand, within BBR_RS11770 at 398.215 kb on - strand, within BBR_RS11770 at 398.238 kb on - strand, within BBR_RS11770 at 398.240 kb on + strand, within BBR_RS11770 at 398.248 kb on - strand, within BBR_RS11770 at 398.255 kb on + strand, within BBR_RS11770 at 398.265 kb on - strand, within BBR_RS11770 at 398.276 kb on + strand, within BBR_RS11770 at 398.371 kb on - strand, within BBR_RS11770 at 398.371 kb on - strand, within BBR_RS11770 at 398.380 kb on + strand at 398.395 kb on + strand at 398.418 kb on + strand at 398.422 kb on - strand at 398.473 kb on + strand at 398.473 kb on + strand at 398.493 kb on + strand at 398.496 kb on - strand at 398.503 kb on + strand at 398.503 kb on + strand at 398.511 kb on - strand at 398.511 kb on - strand at 398.515 kb on + strand at 398.525 kb on + strand at 398.539 kb on - strand at 398.539 kb on - strand at 398.540 kb on + strand at 398.576 kb on + strand at 398.576 kb on + strand at 398.586 kb on + strand at 398.589 kb on - strand at 398.589 kb on - strand at 398.594 kb on - strand at 398.594 kb on - strand at 398.597 kb on + strand at 398.604 kb on - strand at 398.610 kb on + strand at 398.656 kb on - strand at 398.682 kb on + strand at 398.688 kb on - strand at 398.688 kb on - strand at 398.690 kb on - strand at 398.693 kb on + strand at 398.706 kb on - strand at 398.712 kb on - strand at 398.719 kb on + strand at 398.719 kb on + strand at 398.755 kb on + strand at 398.805 kb on + strand, within BBR_RS11775 at 398.805 kb on + strand, within BBR_RS11775 at 398.819 kb on + strand, within BBR_RS11775 at 398.852 kb on - strand, within BBR_RS11775 at 398.853 kb on + strand, within BBR_RS11775 at 398.853 kb on + strand, within BBR_RS11775 at 398.854 kb on + strand, within BBR_RS11775 at 398.861 kb on - strand, within BBR_RS11775 at 398.888 kb on + strand, within BBR_RS11775 at 398.893 kb on + strand, within BBR_RS11775 at 398.893 kb on + strand, within BBR_RS11775 at 398.901 kb on - strand, within BBR_RS11775 at 398.901 kb on - strand, within BBR_RS11775 at 398.903 kb on - strand, within BBR_RS11775 at 398.921 kb on + strand, within BBR_RS11775 at 398.924 kb on + strand, within BBR_RS11775 at 398.940 kb on - strand, within BBR_RS11775 at 398.995 kb on + strand, within BBR_RS11775 at 399.003 kb on + strand, within BBR_RS11775 at 399.003 kb on - strand, within BBR_RS11775 at 399.003 kb on - strand, within BBR_RS11775 at 399.076 kb on + strand, within BBR_RS11775 at 399.087 kb on + strand, within BBR_RS11775 at 399.087 kb on + strand, within BBR_RS11775 at 399.095 kb on - strand, within BBR_RS11775 at 399.101 kb on + strand, within BBR_RS11775 at 399.148 kb on - strand, within BBR_RS11775 at 399.162 kb on + strand, within BBR_RS11775 at 399.164 kb on - strand, within BBR_RS11775 at 399.166 kb on + strand, within BBR_RS11775 at 399.166 kb on + strand, within BBR_RS11775 at 399.166 kb on + strand, within BBR_RS11775 at 399.174 kb on - strand, within BBR_RS11775 at 399.174 kb on - strand, within BBR_RS11775 at 399.174 kb on - strand, within BBR_RS11775 at 399.201 kb on + strand, within BBR_RS11775 at 399.210 kb on + strand, within BBR_RS11775 at 399.218 kb on - strand, within BBR_RS11775 at 399.220 kb on - strand, within BBR_RS11775 at 399.229 kb on + strand, within BBR_RS11775 at 399.231 kb on + strand, within BBR_RS11775 at 399.236 kb on + strand, within BBR_RS11775 at 399.236 kb on + strand, within BBR_RS11775 at 399.236 kb on + strand, within BBR_RS11775 at 399.239 kb on - strand, within BBR_RS11775 at 399.239 kb on - strand, within BBR_RS11775 at 399.257 kb on + strand, within BBR_RS11775 at 399.273 kb on - strand, within BBR_RS11775 at 399.283 kb on + strand, within BBR_RS11775 at 399.310 kb on - strand, within BBR_RS11775 at 399.320 kb on - strand, within BBR_RS11775 at 399.326 kb on + strand, within BBR_RS11775 at 399.328 kb on + strand, within BBR_RS11775 at 399.328 kb on + strand, within BBR_RS11775 at 399.360 kb on + strand, within BBR_RS11775 at 399.382 kb on + strand, within BBR_RS11775 at 399.382 kb on + strand, within BBR_RS11775 at 399.390 kb on - strand, within BBR_RS11775 at 399.390 kb on - strand, within BBR_RS11775 at 399.390 kb on - strand, within BBR_RS11775 at 399.390 kb on - strand, within BBR_RS11775 at 399.396 kb on + strand, within BBR_RS11775 at 399.413 kb on - strand, within BBR_RS11775
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 397,025 - BBR_RS11760 0.57 -0.3 397,039 + BBR_RS11760 0.58 +0.1 397,087 + BBR_RS11760 0.61 -0.1 397,091 + BBR_RS11760 0.61 -0.5 397,124 + BBR_RS11760 0.63 -0.5 397,174 + BBR_RS11760 0.67 +0.6 397,182 + BBR_RS11760 0.67 -0.1 397,182 + BBR_RS11760 0.67 -0.4 397,182 + BBR_RS11760 0.67 +0.4 397,182 + BBR_RS11760 0.67 -0.0 397,190 - BBR_RS11760 0.68 +0.5 397,202 + BBR_RS11760 0.69 -0.2 397,205 - BBR_RS11760 0.69 +0.8 397,223 - BBR_RS11760 0.70 +0.5 397,225 + BBR_RS11760 0.70 +0.3 397,225 + BBR_RS11760 0.70 +0.0 397,255 - BBR_RS11760 0.72 +0.2 397,255 - BBR_RS11760 0.72 +0.4 397,272 + BBR_RS11760 0.74 +1.0 397,292 + BBR_RS11760 0.75 +0.0 397,297 + BBR_RS11760 0.75 -0.5 397,297 + BBR_RS11760 0.75 +0.1 397,305 - BBR_RS11760 0.76 +0.1 397,342 + BBR_RS11760 0.78 +0.3 397,346 - BBR_RS11760 0.79 -0.3 397,405 + BBR_RS11760 0.83 -0.0 397,405 - BBR_RS11760 0.83 +0.0 397,415 + BBR_RS11760 0.83 +0.9 397,433 - BBR_RS11760 0.85 +0.2 397,445 + BBR_RS11760 0.85 -0.5 397,445 + BBR_RS11760 0.85 +0.0 397,453 + BBR_RS11760 0.86 -1.8 397,453 - BBR_RS11760 0.86 +1.4 397,461 - BBR_RS11760 0.86 +0.5 397,469 + BBR_RS11760 0.87 -0.3 397,477 - BBR_RS11760 0.88 +0.1 397,482 - BBR_RS11760 0.88 -0.4 397,482 - BBR_RS11760 0.88 +0.0 397,482 - BBR_RS11760 0.88 -0.3 397,511 - BBR_RS11760 0.90 -0.3 397,516 - +0.5 397,520 - -0.1 397,522 + -1.5 397,530 - +0.1 397,530 - -1.0 397,556 - -0.5 397,556 - +0.3 397,556 - +0.1 397,564 - -0.2 397,571 + -0.1 397,592 + +0.6 397,592 + +0.3 397,602 + -0.7 397,602 - +0.4 397,626 + -1.8 397,636 + -0.2 397,636 + +0.2 397,638 + -0.2 397,644 - -1.0 397,644 - -0.1 397,651 + +2.1 397,651 + +0.3 397,659 - -0.5 397,670 + +0.3 397,684 + +0.6 397,692 - BBR_RS11765 0.12 +0.9 397,702 - BBR_RS11765 0.15 -0.3 397,744 + BBR_RS11765 0.30 +0.5 397,750 + BBR_RS11765 0.32 -0.2 397,758 - BBR_RS11765 0.35 +0.1 397,759 - BBR_RS11765 0.35 +0.3 397,761 + BBR_RS11765 0.36 +0.4 397,776 + BBR_RS11765 0.41 -0.5 397,800 + BBR_RS11765 0.49 +0.2 397,800 + BBR_RS11765 0.49 -0.0 397,808 + BBR_RS11765 0.52 +0.2 397,836 + BBR_RS11765 0.62 +0.3 397,836 + BBR_RS11765 0.62 -0.5 397,836 + BBR_RS11765 0.62 +0.3 397,844 - BBR_RS11765 0.64 +0.3 397,844 - BBR_RS11765 0.64 -0.2 397,851 - BBR_RS11765 0.67 -0.5 397,854 + BBR_RS11765 0.68 -0.5 397,856 + BBR_RS11765 0.68 -0.0 397,867 + BBR_RS11765 0.72 -0.1 397,869 - BBR_RS11765 0.73 -0.1 397,883 - BBR_RS11765 0.78 +0.1 397,890 + BBR_RS11765 0.80 -0.5 397,910 + BBR_RS11765 0.87 -0.3 397,918 - BBR_RS11765 0.90 -0.2 397,942 + -0.6 397,942 + +1.0 397,942 + -0.2 397,950 - -0.1 397,951 + +0.4 397,968 - +0.7 397,998 + -0.3 398,022 + +0.4 398,122 + BBR_RS11770 0.25 -0.4 398,156 + BBR_RS11770 0.34 -0.7 398,171 - BBR_RS11770 0.37 -1.6 398,179 - BBR_RS11770 0.39 +1.4 398,207 + BBR_RS11770 0.47 -0.1 398,215 - BBR_RS11770 0.49 -0.7 398,238 - BBR_RS11770 0.55 -0.5 398,240 + BBR_RS11770 0.55 -0.2 398,248 - BBR_RS11770 0.57 -1.5 398,255 + BBR_RS11770 0.59 -1.2 398,265 - BBR_RS11770 0.62 -1.3 398,276 + BBR_RS11770 0.64 -1.2 398,371 - BBR_RS11770 0.89 +0.1 398,371 - BBR_RS11770 0.89 -0.9 398,380 + +0.1 398,395 + +1.3 398,418 + +0.2 398,422 - +0.3 398,473 + +0.4 398,473 + +1.2 398,493 + +0.5 398,496 - +1.0 398,503 + -0.0 398,503 + -0.5 398,511 - +0.7 398,511 - +0.8 398,515 + +0.4 398,525 + +0.3 398,539 - -0.1 398,539 - +1.0 398,540 + +0.7 398,576 + +0.6 398,576 + +0.0 398,586 + -0.4 398,589 - +0.4 398,589 - +0.0 398,594 - -0.8 398,594 - -0.4 398,597 + -0.1 398,604 - +0.3 398,610 + +0.3 398,656 - +0.6 398,682 + +0.6 398,688 - +0.7 398,688 - +0.1 398,690 - +0.1 398,693 + -0.2 398,706 - +0.5 398,712 - -0.3 398,719 + -0.5 398,719 + +0.2 398,755 + -0.2 398,805 + BBR_RS11775 0.12 +0.5 398,805 + BBR_RS11775 0.12 -0.0 398,819 + BBR_RS11775 0.13 +0.2 398,852 - BBR_RS11775 0.15 -0.1 398,853 + BBR_RS11775 0.15 +0.1 398,853 + BBR_RS11775 0.15 -0.5 398,854 + BBR_RS11775 0.15 +0.4 398,861 - BBR_RS11775 0.15 +0.3 398,888 + BBR_RS11775 0.16 +0.4 398,893 + BBR_RS11775 0.17 -0.1 398,893 + BBR_RS11775 0.17 -0.0 398,901 - BBR_RS11775 0.17 -0.2 398,901 - BBR_RS11775 0.17 +0.3 398,903 - BBR_RS11775 0.17 -0.4 398,921 + BBR_RS11775 0.18 -0.0 398,924 + BBR_RS11775 0.18 -0.2 398,940 - BBR_RS11775 0.19 -0.3 398,995 + BBR_RS11775 0.22 +0.2 399,003 + BBR_RS11775 0.22 -0.5 399,003 - BBR_RS11775 0.22 -0.5 399,003 - BBR_RS11775 0.22 +0.1 399,076 + BBR_RS11775 0.26 +0.6 399,087 + BBR_RS11775 0.26 +0.2 399,087 + BBR_RS11775 0.26 +0.3 399,095 - BBR_RS11775 0.27 +0.3 399,101 + BBR_RS11775 0.27 -0.1 399,148 - BBR_RS11775 0.30 -0.3 399,162 + BBR_RS11775 0.30 +0.7 399,164 - BBR_RS11775 0.30 +0.6 399,166 + BBR_RS11775 0.30 -1.0 399,166 + BBR_RS11775 0.30 -0.2 399,166 + BBR_RS11775 0.30 +0.0 399,174 - BBR_RS11775 0.31 +1.1 399,174 - BBR_RS11775 0.31 +0.6 399,174 - BBR_RS11775 0.31 +0.2 399,201 + BBR_RS11775 0.32 -0.2 399,210 + BBR_RS11775 0.33 +0.1 399,218 - BBR_RS11775 0.33 -0.7 399,220 - BBR_RS11775 0.33 +0.3 399,229 + BBR_RS11775 0.34 +0.3 399,231 + BBR_RS11775 0.34 -0.5 399,236 + BBR_RS11775 0.34 +0.3 399,236 + BBR_RS11775 0.34 +0.4 399,236 + BBR_RS11775 0.34 +0.7 399,239 - BBR_RS11775 0.34 -0.2 399,239 - BBR_RS11775 0.34 -0.2 399,257 + BBR_RS11775 0.35 +0.1 399,273 - BBR_RS11775 0.36 -0.1 399,283 + BBR_RS11775 0.36 +0.3 399,310 - BBR_RS11775 0.38 +0.5 399,320 - BBR_RS11775 0.38 +0.7 399,326 + BBR_RS11775 0.39 -0.5 399,328 + BBR_RS11775 0.39 +0.8 399,328 + BBR_RS11775 0.39 +1.7 399,360 + BBR_RS11775 0.40 -1.5 399,382 + BBR_RS11775 0.41 +1.2 399,382 + BBR_RS11775 0.41 +0.9 399,390 - BBR_RS11775 0.42 -0.4 399,390 - BBR_RS11775 0.42 +0.0 399,390 - BBR_RS11775 0.42 +0.1 399,390 - BBR_RS11775 0.42 -0.0 399,396 + BBR_RS11775 0.42 +0.2 399,413 - BBR_RS11775 0.43 +0.0
Or see this region's nucleotide sequence