Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11580 and BBR_RS11585 are separated by 48 nucleotides BBR_RS11585 and BBR_RS11590 are separated by 57 nucleotides BBR_RS11590 and BBR_RS11595 are separated by 19 nucleotides BBR_RS11595 and BBR_RS11600 are separated by 228 nucleotides BBR_RS11600 and BBR_RS20630 are separated by 229 nucleotides
BBR_RS11580: BBR_RS11580 - 30S ribosomal protein S6, at 357,583 to 357,873
_RS11580
BBR_RS11585: BBR_RS11585 - single-stranded DNA-binding protein, at 357,922 to 358,566
_RS11585
BBR_RS11590: BBR_RS11590 - 30S ribosomal protein S18, at 358,624 to 358,872
_RS11590
BBR_RS11595: BBR_RS11595 - 50S ribosomal protein L9, at 358,892 to 359,338
_RS11595
BBR_RS11600: BBR_RS11600 - tRNA-Lys, at 359,567 to 359,640
_RS11600
BBR_RS20630: BBR_RS20630 - transposase, at 359,870 to 360,107
_RS20630
Position (kb) 358
359 Strain fitness (log2 ratio) -2
-1
0
1 at 358.841 kb on + strand, within BBR_RS11590 at 358.882 kb on + strand at 358.932 kb on + strand at 358.932 kb on + strand at 358.932 kb on + strand at 358.932 kb on + strand at 358.936 kb on + strand at 358.963 kb on + strand, within BBR_RS11595 at 358.999 kb on + strand, within BBR_RS11595 at 359.017 kb on + strand, within BBR_RS11595 at 359.017 kb on + strand, within BBR_RS11595 at 359.017 kb on + strand, within BBR_RS11595 at 359.045 kb on + strand, within BBR_RS11595 at 359.060 kb on + strand, within BBR_RS11595 at 359.112 kb on + strand, within BBR_RS11595 at 359.126 kb on + strand, within BBR_RS11595 at 359.139 kb on + strand, within BBR_RS11595 at 359.196 kb on + strand, within BBR_RS11595 at 359.216 kb on + strand, within BBR_RS11595 at 359.216 kb on + strand, within BBR_RS11595 at 359.219 kb on + strand, within BBR_RS11595 at 359.251 kb on + strand, within BBR_RS11595 at 359.342 kb on + strand at 359.355 kb on + strand at 359.381 kb on + strand at 359.389 kb on + strand at 359.389 kb on + strand at 359.389 kb on + strand at 359.412 kb on + strand at 359.643 kb on + strand at 359.719 kb on - strand at 359.754 kb on + strand at 359.847 kb on + strand at 359.847 kb on + strand at 359.853 kb on + strand at 359.853 kb on + strand at 359.855 kb on - strand at 359.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 358,841 + BBR_RS11590 0.87 -1.3 358,882 + -0.3 358,932 + -2.1 358,932 + +0.4 358,932 + -0.8 358,932 + +1.0 358,936 + +0.6 358,963 + BBR_RS11595 0.16 -0.7 358,999 + BBR_RS11595 0.24 +0.1 359,017 + BBR_RS11595 0.28 -0.0 359,017 + BBR_RS11595 0.28 -0.2 359,017 + BBR_RS11595 0.28 +1.0 359,045 + BBR_RS11595 0.34 +0.9 359,060 + BBR_RS11595 0.38 +1.0 359,112 + BBR_RS11595 0.49 +0.9 359,126 + BBR_RS11595 0.52 +0.3 359,139 + BBR_RS11595 0.55 -0.9 359,196 + BBR_RS11595 0.68 -1.9 359,216 + BBR_RS11595 0.72 +0.4 359,216 + BBR_RS11595 0.72 +1.4 359,219 + BBR_RS11595 0.73 +1.4 359,251 + BBR_RS11595 0.80 +1.3 359,342 + +1.2 359,355 + -0.6 359,381 + -0.5 359,389 + +0.2 359,389 + +0.7 359,389 + +0.4 359,412 + -1.5 359,643 + -0.4 359,719 - +0.8 359,754 + -1.4 359,847 + -0.9 359,847 + +0.0 359,853 + +0.2 359,853 + -0.6 359,855 - -0.2 359,861 - +0.0
Or see this region's nucleotide sequence