Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11235 and BBR_RS11240 are separated by 244 nucleotides BBR_RS11240 and BBR_RS11245 are separated by 322 nucleotides
BBR_RS11235: BBR_RS11235 - hypothetical protein, at 267,061 to 267,408
_RS11235
BBR_RS11240: BBR_RS11240 - GtrA family protein, at 267,653 to 268,189
_RS11240
BBR_RS11245: BBR_RS11245 - MFS transporter, at 268,512 to 269,963
_RS11245
Position (kb) 267
268
269 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 266.686 kb on - strand at 266.778 kb on + strand at 266.778 kb on + strand at 266.787 kb on + strand at 266.787 kb on + strand at 266.871 kb on - strand at 266.879 kb on + strand at 266.880 kb on + strand at 266.888 kb on - strand at 266.900 kb on + strand at 266.913 kb on + strand at 266.976 kb on + strand at 267.003 kb on + strand at 267.007 kb on + strand at 267.007 kb on + strand at 267.007 kb on + strand at 267.013 kb on + strand at 267.013 kb on + strand at 267.013 kb on + strand at 267.015 kb on - strand at 267.015 kb on - strand at 267.021 kb on + strand at 267.021 kb on - strand at 267.021 kb on - strand at 267.040 kb on - strand at 267.040 kb on - strand at 267.043 kb on - strand at 267.050 kb on + strand at 267.051 kb on + strand at 267.075 kb on + strand at 267.076 kb on + strand at 267.076 kb on + strand at 267.116 kb on + strand, within BBR_RS11235 at 267.153 kb on - strand, within BBR_RS11235 at 267.163 kb on - strand, within BBR_RS11235 at 267.178 kb on + strand, within BBR_RS11235 at 267.190 kb on + strand, within BBR_RS11235 at 267.212 kb on + strand, within BBR_RS11235 at 267.220 kb on - strand, within BBR_RS11235 at 267.221 kb on - strand, within BBR_RS11235 at 267.238 kb on - strand, within BBR_RS11235 at 267.238 kb on - strand, within BBR_RS11235 at 267.241 kb on - strand, within BBR_RS11235 at 267.299 kb on + strand, within BBR_RS11235 at 267.299 kb on + strand, within BBR_RS11235 at 267.305 kb on + strand, within BBR_RS11235 at 267.307 kb on - strand, within BBR_RS11235 at 267.308 kb on - strand, within BBR_RS11235 at 267.315 kb on + strand, within BBR_RS11235 at 267.318 kb on - strand, within BBR_RS11235 at 267.324 kb on + strand, within BBR_RS11235 at 267.329 kb on + strand, within BBR_RS11235 at 267.329 kb on + strand, within BBR_RS11235 at 267.334 kb on + strand, within BBR_RS11235 at 267.342 kb on - strand, within BBR_RS11235 at 267.342 kb on - strand, within BBR_RS11235 at 267.342 kb on - strand, within BBR_RS11235 at 267.353 kb on + strand, within BBR_RS11235 at 267.363 kb on + strand, within BBR_RS11235 at 267.363 kb on + strand, within BBR_RS11235 at 267.363 kb on + strand, within BBR_RS11235 at 267.363 kb on + strand, within BBR_RS11235 at 267.363 kb on + strand, within BBR_RS11235 at 267.363 kb on + strand, within BBR_RS11235 at 267.368 kb on + strand, within BBR_RS11235 at 267.371 kb on - strand, within BBR_RS11235 at 267.371 kb on - strand, within BBR_RS11235 at 267.371 kb on - strand, within BBR_RS11235 at 267.371 kb on - strand, within BBR_RS11235 at 267.371 kb on - strand, within BBR_RS11235 at 267.371 kb on - strand, within BBR_RS11235 at 267.376 kb on - strand at 267.376 kb on - strand at 267.382 kb on + strand at 267.390 kb on - strand at 267.391 kb on + strand at 267.412 kb on - strand at 267.455 kb on + strand at 267.458 kb on + strand at 267.464 kb on - strand at 267.468 kb on + strand at 267.468 kb on + strand at 267.468 kb on + strand at 267.468 kb on + strand at 267.468 kb on + strand at 267.468 kb on + strand at 267.468 kb on + strand at 267.476 kb on - strand at 267.508 kb on + strand at 267.508 kb on + strand at 267.515 kb on - strand at 267.516 kb on - strand at 267.516 kb on - strand at 267.516 kb on - strand at 267.516 kb on - strand at 267.518 kb on + strand at 267.537 kb on - strand at 267.540 kb on - strand at 267.545 kb on - strand at 267.568 kb on + strand at 267.590 kb on - strand at 267.590 kb on - strand at 267.595 kb on + strand at 267.602 kb on - strand at 267.602 kb on - strand at 267.605 kb on + strand at 268.067 kb on + strand, within BBR_RS11240 at 268.067 kb on + strand, within BBR_RS11240 at 268.095 kb on + strand, within BBR_RS11240 at 268.098 kb on + strand, within BBR_RS11240 at 268.155 kb on + strand at 268.155 kb on + strand at 268.160 kb on + strand at 268.168 kb on + strand at 268.168 kb on + strand at 268.168 kb on + strand at 268.175 kb on - strand at 268.176 kb on - strand at 268.176 kb on - strand at 268.214 kb on + strand at 268.239 kb on + strand at 268.247 kb on - strand at 268.300 kb on + strand at 268.363 kb on + strand at 268.371 kb on - strand at 268.371 kb on - strand at 268.429 kb on + strand at 268.440 kb on + strand at 268.445 kb on - strand at 268.457 kb on - strand at 268.488 kb on + strand at 268.496 kb on - strand at 268.505 kb on - strand at 268.509 kb on + strand at 268.509 kb on + strand at 268.511 kb on - strand at 268.517 kb on - strand at 268.519 kb on - strand at 268.519 kb on - strand at 268.532 kb on + strand at 268.532 kb on + strand at 268.552 kb on + strand at 268.560 kb on - strand at 268.560 kb on - strand at 268.560 kb on - strand at 268.562 kb on + strand at 268.562 kb on + strand at 268.570 kb on - strand at 268.570 kb on - strand at 268.577 kb on - strand at 268.607 kb on - strand at 268.608 kb on + strand at 268.614 kb on - strand at 268.614 kb on - strand at 268.616 kb on + strand at 268.616 kb on + strand at 268.636 kb on + strand at 268.655 kb on + strand at 268.659 kb on + strand, within BBR_RS11245 at 268.665 kb on + strand, within BBR_RS11245 at 268.665 kb on + strand, within BBR_RS11245 at 268.681 kb on - strand, within BBR_RS11245 at 268.682 kb on + strand, within BBR_RS11245 at 268.699 kb on + strand, within BBR_RS11245 at 268.704 kb on + strand, within BBR_RS11245 at 268.712 kb on - strand, within BBR_RS11245 at 268.712 kb on - strand, within BBR_RS11245 at 268.719 kb on - strand, within BBR_RS11245 at 268.723 kb on + strand, within BBR_RS11245 at 268.725 kb on - strand, within BBR_RS11245 at 268.746 kb on + strand, within BBR_RS11245 at 268.750 kb on + strand, within BBR_RS11245 at 268.750 kb on + strand, within BBR_RS11245 at 268.774 kb on - strand, within BBR_RS11245 at 268.783 kb on + strand, within BBR_RS11245 at 268.787 kb on + strand, within BBR_RS11245 at 268.791 kb on - strand, within BBR_RS11245 at 268.812 kb on - strand, within BBR_RS11245 at 268.869 kb on - strand, within BBR_RS11245 at 268.879 kb on - strand, within BBR_RS11245 at 268.884 kb on + strand, within BBR_RS11245 at 268.891 kb on - strand, within BBR_RS11245 at 268.893 kb on + strand, within BBR_RS11245 at 268.893 kb on + strand, within BBR_RS11245 at 268.926 kb on + strand, within BBR_RS11245 at 268.926 kb on + strand, within BBR_RS11245 at 268.929 kb on + strand, within BBR_RS11245 at 268.932 kb on - strand, within BBR_RS11245 at 268.932 kb on - strand, within BBR_RS11245 at 268.932 kb on - strand, within BBR_RS11245 at 268.934 kb on - strand, within BBR_RS11245 at 268.939 kb on - strand, within BBR_RS11245 at 268.944 kb on - strand, within BBR_RS11245 at 268.949 kb on + strand, within BBR_RS11245 at 268.951 kb on + strand, within BBR_RS11245 at 268.971 kb on + strand, within BBR_RS11245 at 268.971 kb on + strand, within BBR_RS11245 at 268.971 kb on + strand, within BBR_RS11245 at 268.979 kb on - strand, within BBR_RS11245 at 268.979 kb on - strand, within BBR_RS11245 at 268.979 kb on - strand, within BBR_RS11245 at 268.979 kb on - strand, within BBR_RS11245 at 268.981 kb on + strand, within BBR_RS11245 at 268.986 kb on - strand, within BBR_RS11245 at 268.996 kb on - strand, within BBR_RS11245 at 269.022 kb on + strand, within BBR_RS11245 at 269.023 kb on - strand, within BBR_RS11245 at 269.024 kb on + strand, within BBR_RS11245 at 269.032 kb on - strand, within BBR_RS11245 at 269.054 kb on + strand, within BBR_RS11245 at 269.054 kb on + strand, within BBR_RS11245 at 269.056 kb on - strand, within BBR_RS11245 at 269.062 kb on - strand, within BBR_RS11245 at 269.079 kb on + strand, within BBR_RS11245 at 269.097 kb on + strand, within BBR_RS11245 at 269.097 kb on + strand, within BBR_RS11245 at 269.105 kb on + strand, within BBR_RS11245 at 269.163 kb on - strand, within BBR_RS11245 at 269.168 kb on + strand, within BBR_RS11245 at 269.169 kb on + strand, within BBR_RS11245 at 269.177 kb on - strand, within BBR_RS11245
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 266,686 - -0.1 266,778 + +0.5 266,778 + -0.0 266,787 + +0.3 266,787 + -0.4 266,871 - +0.4 266,879 + +0.0 266,880 + +0.2 266,888 - -0.5 266,900 + +0.2 266,913 + +0.2 266,976 + -0.5 267,003 + -0.2 267,007 + +0.0 267,007 + -0.9 267,007 + -0.0 267,013 + +0.5 267,013 + +0.0 267,013 + +0.4 267,015 - +0.7 267,015 - +0.1 267,021 + -0.6 267,021 - +1.1 267,021 - -0.5 267,040 - +0.4 267,040 - +0.6 267,043 - -0.5 267,050 + +0.1 267,051 + -0.5 267,075 + -0.4 267,076 + +0.2 267,076 + -1.5 267,116 + BBR_RS11235 0.16 -1.0 267,153 - BBR_RS11235 0.26 +0.2 267,163 - BBR_RS11235 0.29 +0.2 267,178 + BBR_RS11235 0.34 -1.6 267,190 + BBR_RS11235 0.37 +0.6 267,212 + BBR_RS11235 0.43 -0.0 267,220 - BBR_RS11235 0.46 -1.8 267,221 - BBR_RS11235 0.46 +0.5 267,238 - BBR_RS11235 0.51 +0.1 267,238 - BBR_RS11235 0.51 +0.3 267,241 - BBR_RS11235 0.52 -0.1 267,299 + BBR_RS11235 0.68 +0.3 267,299 + BBR_RS11235 0.68 +0.1 267,305 + BBR_RS11235 0.70 -1.0 267,307 - BBR_RS11235 0.71 +0.4 267,308 - BBR_RS11235 0.71 +0.1 267,315 + BBR_RS11235 0.73 -1.2 267,318 - BBR_RS11235 0.74 +0.9 267,324 + BBR_RS11235 0.76 +0.3 267,329 + BBR_RS11235 0.77 +0.2 267,329 + BBR_RS11235 0.77 -0.8 267,334 + BBR_RS11235 0.78 +0.4 267,342 - BBR_RS11235 0.81 +0.2 267,342 - BBR_RS11235 0.81 -0.3 267,342 - BBR_RS11235 0.81 +0.0 267,353 + BBR_RS11235 0.84 +0.4 267,363 + BBR_RS11235 0.87 +0.9 267,363 + BBR_RS11235 0.87 +0.0 267,363 + BBR_RS11235 0.87 -0.6 267,363 + BBR_RS11235 0.87 +1.3 267,363 + BBR_RS11235 0.87 -0.7 267,363 + BBR_RS11235 0.87 -0.5 267,368 + BBR_RS11235 0.88 +0.2 267,371 - BBR_RS11235 0.89 -0.4 267,371 - BBR_RS11235 0.89 +0.0 267,371 - BBR_RS11235 0.89 -0.2 267,371 - BBR_RS11235 0.89 +0.3 267,371 - BBR_RS11235 0.89 -1.1 267,371 - BBR_RS11235 0.89 -1.2 267,376 - +0.5 267,376 - +0.4 267,382 + +0.7 267,390 - -0.0 267,391 + -1.8 267,412 - +0.0 267,455 + -0.4 267,458 + +0.3 267,464 - -0.3 267,468 + +0.4 267,468 + -0.4 267,468 + -1.9 267,468 + -0.6 267,468 + -0.1 267,468 + +0.6 267,468 + +1.4 267,476 - +0.4 267,508 + -0.4 267,508 + +0.7 267,515 - +0.5 267,516 - +0.3 267,516 - +0.3 267,516 - +0.5 267,516 - +0.0 267,518 + +0.8 267,537 - -1.3 267,540 - +0.7 267,545 - -0.3 267,568 + +0.4 267,590 - +0.8 267,590 - +0.4 267,595 + +0.1 267,602 - -0.1 267,602 - +1.1 267,605 + -2.2 268,067 + BBR_RS11240 0.77 -0.9 268,067 + BBR_RS11240 0.77 -0.2 268,095 + BBR_RS11240 0.82 -1.6 268,098 + BBR_RS11240 0.83 +0.5 268,155 + -0.8 268,155 + -0.4 268,160 + +0.0 268,168 + -2.1 268,168 + -1.7 268,168 + -3.6 268,175 - -2.9 268,176 - -0.1 268,176 - -2.0 268,214 + +0.4 268,239 + +0.6 268,247 - -0.2 268,300 + -0.5 268,363 + -0.2 268,371 - +0.5 268,371 - +0.6 268,429 + +0.2 268,440 + +0.2 268,445 - +0.2 268,457 - +0.4 268,488 + -0.5 268,496 - +0.2 268,505 - +0.2 268,509 + +0.2 268,509 + +0.6 268,511 - -1.7 268,517 - -0.5 268,519 - -0.4 268,519 - -0.2 268,532 + +0.4 268,532 + +0.3 268,552 + -0.0 268,560 - +0.1 268,560 - +0.1 268,560 - +1.3 268,562 + +0.6 268,562 + +0.3 268,570 - +0.4 268,570 - +0.4 268,577 - +0.3 268,607 - +0.4 268,608 + -0.4 268,614 - -0.4 268,614 - +0.5 268,616 + -1.6 268,616 + -0.2 268,636 + -0.4 268,655 + +0.8 268,659 + BBR_RS11245 0.10 -0.3 268,665 + BBR_RS11245 0.11 +0.1 268,665 + BBR_RS11245 0.11 +0.2 268,681 - BBR_RS11245 0.12 -0.6 268,682 + BBR_RS11245 0.12 -0.1 268,699 + BBR_RS11245 0.13 -0.1 268,704 + BBR_RS11245 0.13 -0.3 268,712 - BBR_RS11245 0.14 +0.9 268,712 - BBR_RS11245 0.14 +0.4 268,719 - BBR_RS11245 0.14 -0.1 268,723 + BBR_RS11245 0.15 -0.0 268,725 - BBR_RS11245 0.15 +0.3 268,746 + BBR_RS11245 0.16 +0.2 268,750 + BBR_RS11245 0.16 +0.2 268,750 + BBR_RS11245 0.16 +0.2 268,774 - BBR_RS11245 0.18 +0.6 268,783 + BBR_RS11245 0.19 +1.0 268,787 + BBR_RS11245 0.19 +0.3 268,791 - BBR_RS11245 0.19 +0.4 268,812 - BBR_RS11245 0.21 +0.1 268,869 - BBR_RS11245 0.25 -0.7 268,879 - BBR_RS11245 0.25 +0.2 268,884 + BBR_RS11245 0.26 -0.2 268,891 - BBR_RS11245 0.26 +0.3 268,893 + BBR_RS11245 0.26 +0.7 268,893 + BBR_RS11245 0.26 +0.4 268,926 + BBR_RS11245 0.29 +0.4 268,926 + BBR_RS11245 0.29 -1.4 268,929 + BBR_RS11245 0.29 +0.6 268,932 - BBR_RS11245 0.29 +0.2 268,932 - BBR_RS11245 0.29 +0.5 268,932 - BBR_RS11245 0.29 +0.7 268,934 - BBR_RS11245 0.29 -0.4 268,939 - BBR_RS11245 0.29 +0.3 268,944 - BBR_RS11245 0.30 +0.0 268,949 + BBR_RS11245 0.30 +0.0 268,951 + BBR_RS11245 0.30 -0.3 268,971 + BBR_RS11245 0.32 +0.2 268,971 + BBR_RS11245 0.32 -0.3 268,971 + BBR_RS11245 0.32 -0.7 268,979 - BBR_RS11245 0.32 +0.7 268,979 - BBR_RS11245 0.32 -0.2 268,979 - BBR_RS11245 0.32 +0.9 268,979 - BBR_RS11245 0.32 -0.2 268,981 + BBR_RS11245 0.32 -0.9 268,986 - BBR_RS11245 0.33 -0.5 268,996 - BBR_RS11245 0.33 -0.3 269,022 + BBR_RS11245 0.35 -1.2 269,023 - BBR_RS11245 0.35 +0.0 269,024 + BBR_RS11245 0.35 -0.4 269,032 - BBR_RS11245 0.36 -0.0 269,054 + BBR_RS11245 0.37 +0.8 269,054 + BBR_RS11245 0.37 +0.6 269,056 - BBR_RS11245 0.37 +0.5 269,062 - BBR_RS11245 0.38 -0.5 269,079 + BBR_RS11245 0.39 +0.8 269,097 + BBR_RS11245 0.40 +0.3 269,097 + BBR_RS11245 0.40 +0.3 269,105 + BBR_RS11245 0.41 +0.2 269,163 - BBR_RS11245 0.45 -0.3 269,168 + BBR_RS11245 0.45 -0.3 269,169 + BBR_RS11245 0.45 +0.4 269,177 - BBR_RS11245 0.46 +0.7
Or see this region's nucleotide sequence