Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11200 and BBR_RS11205 are separated by 123 nucleotides BBR_RS11205 and BBR_RS11210 are separated by 257 nucleotides BBR_RS11210 and BBR_RS11215 are separated by 852 nucleotides
BBR_RS11200: BBR_RS11200 - LacI family transcriptional regulator, at 257,965 to 258,594
_RS11200
BBR_RS11205: BBR_RS11205 - HPr family phosphocarrier protein, at 258,718 to 258,978
_RS11205
BBR_RS11210: BBR_RS11210 - phosphoenolpyruvate--protein phosphotransferase, at 259,236 to 260,885
_RS11210
BBR_RS11215: BBR_RS11215 - hypothetical protein, at 261,738 to 262,544
_RS11215
Position (kb) 259
260
261 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 258.243 kb on - strand, within BBR_RS11200 at 258.261 kb on + strand, within BBR_RS11200 at 258.273 kb on + strand, within BBR_RS11200 at 258.294 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.359 kb on - strand, within BBR_RS11200 at 258.377 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.397 kb on + strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.440 kb on - strand, within BBR_RS11200 at 258.465 kb on + strand, within BBR_RS11200 at 258.503 kb on + strand, within BBR_RS11200 at 258.508 kb on + strand, within BBR_RS11200 at 258.516 kb on - strand, within BBR_RS11200 at 258.516 kb on - strand, within BBR_RS11200 at 258.540 kb on + strand at 258.553 kb on + strand at 258.571 kb on - strand at 258.571 kb on - strand at 258.598 kb on - strand at 258.653 kb on + strand at 258.745 kb on - strand, within BBR_RS11205 at 258.797 kb on + strand, within BBR_RS11205 at 258.797 kb on + strand, within BBR_RS11205 at 258.805 kb on - strand, within BBR_RS11205 at 258.805 kb on - strand, within BBR_RS11205 at 258.912 kb on - strand, within BBR_RS11205 at 258.961 kb on - strand at 258.983 kb on - strand at 258.983 kb on - strand at 258.997 kb on - strand at 259.045 kb on - strand at 259.045 kb on - strand at 259.045 kb on - strand at 259.046 kb on + strand at 259.052 kb on - strand at 259.087 kb on + strand at 259.101 kb on - strand at 259.162 kb on + strand at 259.170 kb on + strand at 259.170 kb on + strand at 259.183 kb on + strand at 259.211 kb on - strand at 259.213 kb on - strand at 259.214 kb on - strand at 259.214 kb on - strand at 259.231 kb on + strand at 259.347 kb on - strand at 259.481 kb on - strand, within BBR_RS11210 at 259.523 kb on + strand, within BBR_RS11210 at 259.567 kb on - strand, within BBR_RS11210 at 259.575 kb on + strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.657 kb on - strand, within BBR_RS11210 at 259.667 kb on - strand, within BBR_RS11210 at 259.669 kb on - strand, within BBR_RS11210 at 259.767 kb on + strand, within BBR_RS11210 at 259.768 kb on - strand, within BBR_RS11210 at 259.768 kb on - strand, within BBR_RS11210 at 259.869 kb on + strand, within BBR_RS11210 at 259.874 kb on + strand, within BBR_RS11210 at 259.874 kb on + strand, within BBR_RS11210 at 259.890 kb on - strand, within BBR_RS11210 at 259.897 kb on - strand, within BBR_RS11210 at 259.983 kb on + strand, within BBR_RS11210 at 259.985 kb on - strand, within BBR_RS11210 at 260.018 kb on + strand, within BBR_RS11210 at 260.047 kb on - strand, within BBR_RS11210 at 260.076 kb on + strand, within BBR_RS11210 at 260.084 kb on - strand, within BBR_RS11210 at 260.084 kb on - strand, within BBR_RS11210 at 260.105 kb on - strand, within BBR_RS11210 at 260.106 kb on - strand, within BBR_RS11210 at 260.111 kb on - strand, within BBR_RS11210 at 260.137 kb on + strand, within BBR_RS11210 at 260.165 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.179 kb on - strand, within BBR_RS11210 at 260.192 kb on - strand, within BBR_RS11210 at 260.194 kb on - strand, within BBR_RS11210 at 260.211 kb on + strand, within BBR_RS11210 at 260.295 kb on - strand, within BBR_RS11210 at 260.301 kb on + strand, within BBR_RS11210 at 260.311 kb on + strand, within BBR_RS11210 at 260.311 kb on + strand, within BBR_RS11210 at 260.353 kb on - strand, within BBR_RS11210 at 260.365 kb on - strand, within BBR_RS11210 at 260.370 kb on + strand, within BBR_RS11210 at 260.375 kb on + strand, within BBR_RS11210 at 260.409 kb on - strand, within BBR_RS11210 at 260.409 kb on - strand, within BBR_RS11210 at 260.410 kb on - strand, within BBR_RS11210 at 260.473 kb on - strand, within BBR_RS11210 at 260.551 kb on + strand, within BBR_RS11210 at 260.612 kb on + strand, within BBR_RS11210 at 260.620 kb on - strand, within BBR_RS11210 at 260.655 kb on - strand, within BBR_RS11210 at 260.691 kb on - strand, within BBR_RS11210 at 260.702 kb on + strand, within BBR_RS11210 at 260.765 kb on + strand at 260.834 kb on + strand at 260.855 kb on + strand at 260.862 kb on + strand at 260.864 kb on + strand at 260.864 kb on + strand at 260.864 kb on + strand at 260.909 kb on - strand at 260.929 kb on + strand at 260.929 kb on + strand at 260.989 kb on + strand at 261.054 kb on - strand at 261.093 kb on - strand at 261.100 kb on + strand at 261.101 kb on - strand at 261.101 kb on - strand at 261.101 kb on - strand at 261.110 kb on - strand at 261.110 kb on - strand at 261.129 kb on - strand at 261.129 kb on - strand at 261.159 kb on - strand at 261.180 kb on - strand at 261.180 kb on - strand at 261.182 kb on + strand at 261.190 kb on - strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.250 kb on + strand at 261.255 kb on + strand at 261.264 kb on - strand at 261.264 kb on - strand at 261.267 kb on + strand at 261.270 kb on - strand at 261.270 kb on - strand at 261.270 kb on - strand at 261.292 kb on + strand at 261.292 kb on + strand at 261.298 kb on + strand at 261.305 kb on + strand at 261.308 kb on + strand at 261.308 kb on + strand at 261.316 kb on - strand at 261.323 kb on - strand at 261.323 kb on - strand at 261.346 kb on + strand at 261.354 kb on - strand at 261.354 kb on - strand at 261.354 kb on - strand at 261.354 kb on - strand at 261.364 kb on - strand at 261.364 kb on - strand at 261.381 kb on - strand at 261.388 kb on - strand at 261.394 kb on - strand at 261.394 kb on - strand at 261.427 kb on + strand at 261.427 kb on + strand at 261.430 kb on - strand at 261.431 kb on - strand at 261.431 kb on - strand at 261.433 kb on + strand at 261.435 kb on - strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.440 kb on + strand at 261.440 kb on + strand at 261.440 kb on + strand at 261.447 kb on - strand at 261.447 kb on - strand at 261.448 kb on - strand at 261.448 kb on - strand at 261.448 kb on - strand at 261.453 kb on + strand at 261.461 kb on - strand at 261.466 kb on - strand at 261.523 kb on - strand at 261.551 kb on - strand at 261.564 kb on + strand at 261.564 kb on + strand at 261.566 kb on + strand at 261.569 kb on + strand at 261.572 kb on - strand at 261.572 kb on - strand at 261.579 kb on - strand at 261.582 kb on - strand at 261.585 kb on - strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.600 kb on - strand at 261.607 kb on - strand at 261.607 kb on - strand at 261.612 kb on + strand at 261.624 kb on - strand at 261.677 kb on + strand at 261.695 kb on + strand at 261.695 kb on + strand at 261.695 kb on + strand at 261.695 kb on + strand at 261.710 kb on - strand at 261.753 kb on + strand at 261.760 kb on + strand at 261.767 kb on - strand at 261.770 kb on + strand at 261.777 kb on + strand at 261.777 kb on - strand at 261.777 kb on - strand at 261.777 kb on - strand at 261.813 kb on - strand at 261.813 kb on - strand at 261.820 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.836 kb on - strand, within BBR_RS11215 at 261.836 kb on - strand, within BBR_RS11215 at 261.844 kb on - strand, within BBR_RS11215 at 261.846 kb on + strand, within BBR_RS11215 at 261.851 kb on + strand, within BBR_RS11215 at 261.851 kb on + strand, within BBR_RS11215 at 261.854 kb on - strand, within BBR_RS11215 at 261.854 kb on - strand, within BBR_RS11215 at 261.858 kb on + strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.871 kb on - strand, within BBR_RS11215 at 261.876 kb on - strand, within BBR_RS11215
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 258,243 - BBR_RS11200 0.44 +1.3 258,261 + BBR_RS11200 0.47 +1.1 258,273 + BBR_RS11200 0.49 +0.4 258,294 + BBR_RS11200 0.52 +0.6 258,318 + BBR_RS11200 0.56 -0.6 258,318 + BBR_RS11200 0.56 +0.5 258,318 + BBR_RS11200 0.56 -0.9 258,318 + BBR_RS11200 0.56 -0.6 258,318 + BBR_RS11200 0.56 +0.2 258,318 + BBR_RS11200 0.56 -0.5 258,326 - BBR_RS11200 0.57 -0.5 258,326 - BBR_RS11200 0.57 +0.2 258,326 - BBR_RS11200 0.57 +0.4 258,326 - BBR_RS11200 0.57 +0.3 258,326 - BBR_RS11200 0.57 +0.5 258,359 - BBR_RS11200 0.63 +0.1 258,377 - BBR_RS11200 0.65 +0.0 258,391 - BBR_RS11200 0.68 +0.6 258,391 - BBR_RS11200 0.68 +0.3 258,391 - BBR_RS11200 0.68 +0.3 258,391 - BBR_RS11200 0.68 +0.3 258,397 + BBR_RS11200 0.69 -0.2 258,403 - BBR_RS11200 0.70 +0.3 258,403 - BBR_RS11200 0.70 +0.7 258,403 - BBR_RS11200 0.70 +1.1 258,403 - BBR_RS11200 0.70 +0.9 258,403 - BBR_RS11200 0.70 -0.3 258,403 - BBR_RS11200 0.70 -0.0 258,432 + BBR_RS11200 0.74 -0.1 258,432 + BBR_RS11200 0.74 +0.4 258,432 + BBR_RS11200 0.74 -0.2 258,440 - BBR_RS11200 0.75 +0.0 258,465 + BBR_RS11200 0.79 +0.7 258,503 + BBR_RS11200 0.85 -0.1 258,508 + BBR_RS11200 0.86 -0.1 258,516 - BBR_RS11200 0.87 +0.4 258,516 - BBR_RS11200 0.87 -0.2 258,540 + +0.5 258,553 + -0.4 258,571 - -0.0 258,571 - -0.5 258,598 - -0.5 258,653 + -0.2 258,745 - BBR_RS11205 0.10 -2.3 258,797 + BBR_RS11205 0.30 -2.0 258,797 + BBR_RS11205 0.30 -2.1 258,805 - BBR_RS11205 0.33 -3.4 258,805 - BBR_RS11205 0.33 -4.3 258,912 - BBR_RS11205 0.74 -4.1 258,961 - -1.4 258,983 - -1.4 258,983 - -4.1 258,997 - +0.5 259,045 - -0.2 259,045 - -0.3 259,045 - -0.6 259,046 + -0.0 259,052 - -1.1 259,087 + -0.0 259,101 - +0.4 259,162 + -0.6 259,170 + -0.3 259,170 + -0.2 259,183 + +0.9 259,211 - -0.4 259,213 - -0.3 259,214 - -0.8 259,214 - -1.7 259,231 + -4.8 259,347 - -1.3 259,481 - BBR_RS11210 0.15 -1.1 259,523 + BBR_RS11210 0.17 -4.4 259,567 - BBR_RS11210 0.20 -3.0 259,575 + BBR_RS11210 0.21 -2.9 259,644 - BBR_RS11210 0.25 -1.0 259,644 - BBR_RS11210 0.25 -3.7 259,644 - BBR_RS11210 0.25 -4.3 259,657 - BBR_RS11210 0.26 -4.0 259,667 - BBR_RS11210 0.26 -5.2 259,669 - BBR_RS11210 0.26 -2.0 259,767 + BBR_RS11210 0.32 -3.5 259,768 - BBR_RS11210 0.32 -0.7 259,768 - BBR_RS11210 0.32 -5.5 259,869 + BBR_RS11210 0.38 -4.9 259,874 + BBR_RS11210 0.39 -2.7 259,874 + BBR_RS11210 0.39 -3.9 259,890 - BBR_RS11210 0.40 -3.4 259,897 - BBR_RS11210 0.40 -4.1 259,983 + BBR_RS11210 0.45 -4.1 259,985 - BBR_RS11210 0.45 -3.0 260,018 + BBR_RS11210 0.47 -4.4 260,047 - BBR_RS11210 0.49 -4.1 260,076 + BBR_RS11210 0.51 -4.3 260,084 - BBR_RS11210 0.51 -3.5 260,084 - BBR_RS11210 0.51 -4.3 260,105 - BBR_RS11210 0.53 -1.7 260,106 - BBR_RS11210 0.53 -1.9 260,111 - BBR_RS11210 0.53 -2.9 260,137 + BBR_RS11210 0.55 -2.8 260,165 + BBR_RS11210 0.56 -1.4 260,171 + BBR_RS11210 0.57 -4.6 260,171 + BBR_RS11210 0.57 -3.3 260,171 + BBR_RS11210 0.57 -3.6 260,171 + BBR_RS11210 0.57 -2.2 260,171 + BBR_RS11210 0.57 -4.1 260,171 + BBR_RS11210 0.57 -2.3 260,179 - BBR_RS11210 0.57 -3.7 260,192 - BBR_RS11210 0.58 -2.5 260,194 - BBR_RS11210 0.58 -4.6 260,211 + BBR_RS11210 0.59 -4.0 260,295 - BBR_RS11210 0.64 -0.5 260,301 + BBR_RS11210 0.65 -1.8 260,311 + BBR_RS11210 0.65 -3.0 260,311 + BBR_RS11210 0.65 -3.0 260,353 - BBR_RS11210 0.68 -4.8 260,365 - BBR_RS11210 0.68 -2.2 260,370 + BBR_RS11210 0.69 -4.1 260,375 + BBR_RS11210 0.69 -3.2 260,409 - BBR_RS11210 0.71 -4.5 260,409 - BBR_RS11210 0.71 -4.3 260,410 - BBR_RS11210 0.71 +0.5 260,473 - BBR_RS11210 0.75 -1.3 260,551 + BBR_RS11210 0.80 -2.6 260,612 + BBR_RS11210 0.83 -3.8 260,620 - BBR_RS11210 0.84 -3.0 260,655 - BBR_RS11210 0.86 -3.8 260,691 - BBR_RS11210 0.88 -4.3 260,702 + BBR_RS11210 0.89 -4.8 260,765 + -4.0 260,834 + -4.9 260,855 + -0.2 260,862 + -0.1 260,864 + -0.4 260,864 + -0.2 260,864 + +0.0 260,909 - +0.2 260,929 + -0.9 260,929 + -0.1 260,989 + -0.7 261,054 - +0.7 261,093 - +0.4 261,100 + -0.5 261,101 - -0.4 261,101 - +0.8 261,101 - -0.2 261,110 - +0.2 261,110 - -0.2 261,129 - +0.6 261,129 - +0.7 261,159 - +0.7 261,180 - -0.2 261,180 - -1.2 261,182 + +0.2 261,190 - +0.2 261,220 + +0.3 261,220 + -1.3 261,250 + -0.2 261,255 + +0.2 261,264 - -0.7 261,264 - +0.6 261,267 + -0.0 261,270 - -0.2 261,270 - -0.2 261,270 - +0.3 261,292 + +1.1 261,292 + -0.1 261,298 + +0.1 261,305 + -0.3 261,308 + +0.0 261,308 + -0.1 261,316 - -0.7 261,323 - +0.3 261,323 - +0.5 261,346 + +0.1 261,354 - -0.2 261,354 - -0.3 261,354 - +0.2 261,354 - +0.8 261,364 - +0.5 261,364 - -0.4 261,381 - +0.4 261,388 - +0.0 261,394 - -0.1 261,394 - +0.0 261,427 + -0.2 261,427 + +0.1 261,430 - +0.2 261,431 - +0.4 261,431 - -0.0 261,433 + +0.1 261,435 - +0.6 261,439 + -0.2 261,439 + +0.1 261,439 + +0.2 261,439 + -0.2 261,439 + +0.0 261,439 + -0.5 261,440 + -0.6 261,440 + -0.0 261,440 + -0.5 261,447 - +0.6 261,447 - +0.5 261,448 - +0.5 261,448 - +0.4 261,448 - -0.4 261,453 + -0.4 261,461 - -0.2 261,466 - -0.0 261,523 - -0.5 261,551 - -0.3 261,564 + +0.3 261,564 + +0.5 261,566 + +0.6 261,569 + -0.4 261,572 - -1.1 261,572 - +0.3 261,579 - -1.0 261,582 - +0.0 261,585 - +0.5 261,592 + -0.2 261,592 + +0.4 261,592 + +0.2 261,592 + -0.4 261,592 + -0.0 261,600 - +0.1 261,607 - +0.0 261,607 - -0.2 261,612 + -1.0 261,624 - -0.0 261,677 + +0.7 261,695 + +0.3 261,695 + -0.4 261,695 + +0.2 261,695 + -1.0 261,710 - +0.1 261,753 + -0.3 261,760 + -0.6 261,767 - +0.2 261,770 + +0.3 261,777 + -0.3 261,777 - -0.4 261,777 - -0.8 261,777 - -0.0 261,813 - +1.5 261,813 - +1.2 261,820 + BBR_RS11215 0.10 +0.3 261,825 + BBR_RS11215 0.11 +0.4 261,825 + BBR_RS11215 0.11 +0.3 261,825 + BBR_RS11215 0.11 -0.5 261,825 + BBR_RS11215 0.11 +1.3 261,825 + BBR_RS11215 0.11 +0.1 261,825 + BBR_RS11215 0.11 -0.3 261,825 + BBR_RS11215 0.11 +0.8 261,833 - BBR_RS11215 0.12 +0.2 261,833 - BBR_RS11215 0.12 +0.6 261,833 - BBR_RS11215 0.12 -0.6 261,833 - BBR_RS11215 0.12 +0.1 261,833 - BBR_RS11215 0.12 -0.1 261,833 - BBR_RS11215 0.12 -0.8 261,833 - BBR_RS11215 0.12 +0.0 261,836 - BBR_RS11215 0.12 +0.2 261,836 - BBR_RS11215 0.12 +0.6 261,844 - BBR_RS11215 0.13 +0.3 261,846 + BBR_RS11215 0.13 -0.5 261,851 + BBR_RS11215 0.14 +0.2 261,851 + BBR_RS11215 0.14 +0.3 261,854 - BBR_RS11215 0.14 -0.2 261,854 - BBR_RS11215 0.14 -0.7 261,858 + BBR_RS11215 0.15 -0.4 261,859 - BBR_RS11215 0.15 +1.5 261,859 - BBR_RS11215 0.15 +0.5 261,859 - BBR_RS11215 0.15 -1.3 261,859 - BBR_RS11215 0.15 +0.7 261,859 - BBR_RS11215 0.15 +2.0 261,859 - BBR_RS11215 0.15 +0.6 261,871 - BBR_RS11215 0.16 +0.3 261,876 - BBR_RS11215 0.17 -0.6
Or see this region's nucleotide sequence