Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10900 and BBR_RS10905 overlap by 1 nucleotides BBR_RS10905 and BBR_RS10910 are separated by 178 nucleotides
BBR_RS10900: BBR_RS10900 - molecular chaperone DnaK, at 188,855 to 190,735
_RS10900
BBR_RS10905: BBR_RS10905 - nucleotide exchange factor GrpE, at 190,735 to 191,418
_RS10905
BBR_RS10910: BBR_RS10910 - molecular chaperone DnaJ, at 191,597 to 192,610
_RS10910
Position (kb)
190
191
192 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 190.516 kb on + strand, within BBR_RS10900 at 190.516 kb on + strand, within BBR_RS10900 at 190.516 kb on + strand, within BBR_RS10900 at 190.522 kb on + strand, within BBR_RS10900 at 190.530 kb on + strand, within BBR_RS10900 at 190.540 kb on + strand, within BBR_RS10900 at 190.540 kb on + strand, within BBR_RS10900 at 190.549 kb on + strand at 190.555 kb on + strand at 190.555 kb on + strand at 190.569 kb on + strand at 190.599 kb on + strand at 190.599 kb on + strand at 190.611 kb on + strand at 190.611 kb on + strand at 190.815 kb on + strand, within BBR_RS10905 at 190.815 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.998 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.108 kb on + strand, within BBR_RS10905 at 191.305 kb on + strand, within BBR_RS10905 at 191.390 kb on + strand at 191.395 kb on + strand at 191.422 kb on + strand at 191.425 kb on - strand at 191.425 kb on - strand at 191.433 kb on + strand at 191.472 kb on - strand at 191.478 kb on + strand at 191.478 kb on + strand at 191.484 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.499 kb on + strand at 191.499 kb on + strand at 191.507 kb on - strand at 191.519 kb on - strand at 191.527 kb on + strand at 191.535 kb on - strand at 191.543 kb on - strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.553 kb on - strand at 191.553 kb on - strand at 191.567 kb on + strand at 191.587 kb on + strand at 191.603 kb on + strand at 191.608 kb on - strand at 191.619 kb on + strand at 191.626 kb on + strand at 191.626 kb on + strand at 191.636 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.655 kb on - strand at 191.655 kb on - strand at 191.664 kb on - strand at 191.701 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.710 kb on + strand, within BBR_RS10910 at 191.712 kb on - strand, within BBR_RS10910 at 191.719 kb on + strand, within BBR_RS10910 at 191.746 kb on + strand, within BBR_RS10910 at 191.757 kb on + strand, within BBR_RS10910 at 191.765 kb on + strand, within BBR_RS10910 at 191.777 kb on - strand, within BBR_RS10910 at 191.782 kb on - strand, within BBR_RS10910 at 191.784 kb on + strand, within BBR_RS10910 at 191.825 kb on + strand, within BBR_RS10910 at 191.829 kb on - strand, within BBR_RS10910 at 191.861 kb on + strand, within BBR_RS10910 at 191.877 kb on + strand, within BBR_RS10910 at 191.886 kb on + strand, within BBR_RS10910 at 191.896 kb on + strand, within BBR_RS10910 at 191.925 kb on - strand, within BBR_RS10910 at 191.970 kb on + strand, within BBR_RS10910 at 191.970 kb on + strand, within BBR_RS10910 at 191.971 kb on + strand, within BBR_RS10910 at 191.998 kb on + strand, within BBR_RS10910 at 192.014 kb on + strand, within BBR_RS10910 at 192.016 kb on + strand, within BBR_RS10910 at 192.043 kb on + strand, within BBR_RS10910 at 192.044 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.057 kb on - strand, within BBR_RS10910 at 192.069 kb on + strand, within BBR_RS10910 at 192.080 kb on + strand, within BBR_RS10910 at 192.104 kb on + strand, within BBR_RS10910 at 192.106 kb on + strand, within BBR_RS10910 at 192.123 kb on + strand, within BBR_RS10910 at 192.133 kb on + strand, within BBR_RS10910 at 192.142 kb on + strand, within BBR_RS10910 at 192.142 kb on + strand, within BBR_RS10910 at 192.142 kb on + strand, within BBR_RS10910 at 192.148 kb on + strand, within BBR_RS10910 at 192.160 kb on + strand, within BBR_RS10910 at 192.184 kb on + strand, within BBR_RS10910 at 192.184 kb on + strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.226 kb on + strand, within BBR_RS10910 at 192.226 kb on + strand, within BBR_RS10910 at 192.244 kb on + strand, within BBR_RS10910 at 192.252 kb on - strand, within BBR_RS10910 at 192.330 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.355 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.364 kb on + strand, within BBR_RS10910 at 192.365 kb on + strand, within BBR_RS10910 at 192.368 kb on - strand, within BBR_RS10910 at 192.368 kb on - strand, within BBR_RS10910 at 192.368 kb on - strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.400 kb on + strand, within BBR_RS10910
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 190,516 + BBR_RS10900 0.88 +2.5 190,516 + BBR_RS10900 0.88 -0.4 190,516 + BBR_RS10900 0.88 +0.1 190,522 + BBR_RS10900 0.89 -0.1 190,530 + BBR_RS10900 0.89 +1.5 190,540 + BBR_RS10900 0.90 +4.1 190,540 + BBR_RS10900 0.90 +0.9 190,549 + +0.9 190,555 + +0.9 190,555 + -0.5 190,569 + +0.8 190,599 + +0.5 190,599 + +0.2 190,611 + +2.1 190,611 + +0.4 190,815 + BBR_RS10905 0.12 -0.3 190,815 + BBR_RS10905 0.12 -0.2 190,845 + BBR_RS10905 0.16 -0.3 190,845 + BBR_RS10905 0.16 -0.2 190,845 + BBR_RS10905 0.16 -1.5 190,860 + BBR_RS10905 0.18 -2.1 190,860 + BBR_RS10905 0.18 -0.2 190,998 + BBR_RS10905 0.38 -0.6 191,022 + BBR_RS10905 0.42 -0.1 191,022 + BBR_RS10905 0.42 -1.1 191,022 + BBR_RS10905 0.42 -0.1 191,022 + BBR_RS10905 0.42 +0.5 191,108 + BBR_RS10905 0.55 +1.5 191,305 + BBR_RS10905 0.83 +0.4 191,390 + +0.0 191,395 + +0.9 191,422 + -0.1 191,425 - -0.8 191,425 - -0.8 191,433 + -0.7 191,472 - +0.2 191,478 + +0.6 191,478 + +0.6 191,484 + -0.5 191,488 + -0.4 191,488 + +0.3 191,488 + +0.4 191,488 + -0.9 191,488 + -0.1 191,496 - +0.2 191,496 - -0.3 191,496 - -0.3 191,499 + +0.2 191,499 + +0.8 191,507 - -1.1 191,519 - +0.6 191,527 + -0.3 191,535 - -0.7 191,543 - +0.4 191,545 + -0.4 191,545 + -0.4 191,545 + +0.5 191,545 + +0.5 191,545 + -0.3 191,553 - +0.4 191,553 - -0.5 191,567 + +0.4 191,587 + -0.4 191,603 + -0.2 191,608 - +0.4 191,619 + +0.4 191,626 + -0.4 191,626 + -0.9 191,636 + +0.7 191,647 + +0.4 191,647 + -1.0 191,647 + +0.5 191,655 - +0.4 191,655 - +1.1 191,664 - +0.0 191,701 + BBR_RS10910 0.10 -0.7 191,704 + BBR_RS10910 0.11 +0.3 191,704 + BBR_RS10910 0.11 -0.2 191,704 + BBR_RS10910 0.11 -0.5 191,704 + BBR_RS10910 0.11 +1.0 191,704 + BBR_RS10910 0.11 +2.1 191,704 + BBR_RS10910 0.11 -0.2 191,709 + BBR_RS10910 0.11 +0.4 191,709 + BBR_RS10910 0.11 -0.3 191,709 + BBR_RS10910 0.11 -0.4 191,710 + BBR_RS10910 0.11 -1.2 191,712 - BBR_RS10910 0.11 +0.5 191,719 + BBR_RS10910 0.12 +0.4 191,746 + BBR_RS10910 0.15 +0.7 191,757 + BBR_RS10910 0.16 -0.1 191,765 + BBR_RS10910 0.17 -0.7 191,777 - BBR_RS10910 0.18 +1.8 191,782 - BBR_RS10910 0.18 +2.5 191,784 + BBR_RS10910 0.18 +1.3 191,825 + BBR_RS10910 0.22 +0.4 191,829 - BBR_RS10910 0.23 +1.3 191,861 + BBR_RS10910 0.26 -0.5 191,877 + BBR_RS10910 0.28 -0.7 191,886 + BBR_RS10910 0.29 +0.2 191,896 + BBR_RS10910 0.29 -2.7 191,925 - BBR_RS10910 0.32 +1.2 191,970 + BBR_RS10910 0.37 +0.2 191,970 + BBR_RS10910 0.37 +0.3 191,971 + BBR_RS10910 0.37 +0.3 191,998 + BBR_RS10910 0.40 -0.5 192,014 + BBR_RS10910 0.41 -0.5 192,016 + BBR_RS10910 0.41 +0.3 192,043 + BBR_RS10910 0.44 +0.7 192,044 + BBR_RS10910 0.44 -0.6 192,049 + BBR_RS10910 0.45 +0.9 192,049 + BBR_RS10910 0.45 -0.2 192,049 + BBR_RS10910 0.45 -0.2 192,049 + BBR_RS10910 0.45 +0.7 192,057 - BBR_RS10910 0.45 +1.1 192,069 + BBR_RS10910 0.47 +1.4 192,080 + BBR_RS10910 0.48 -0.7 192,104 + BBR_RS10910 0.50 +0.7 192,106 + BBR_RS10910 0.50 -0.5 192,123 + BBR_RS10910 0.52 +0.5 192,133 + BBR_RS10910 0.53 +0.1 192,142 + BBR_RS10910 0.54 -0.3 192,142 + BBR_RS10910 0.54 +0.6 192,142 + BBR_RS10910 0.54 -0.1 192,148 + BBR_RS10910 0.54 +0.3 192,160 + BBR_RS10910 0.56 +0.2 192,184 + BBR_RS10910 0.58 -0.4 192,184 + BBR_RS10910 0.58 +0.5 192,192 - BBR_RS10910 0.59 +0.3 192,192 - BBR_RS10910 0.59 -0.6 192,192 - BBR_RS10910 0.59 +0.1 192,192 - BBR_RS10910 0.59 +1.5 192,226 + BBR_RS10910 0.62 +0.2 192,226 + BBR_RS10910 0.62 -0.2 192,244 + BBR_RS10910 0.64 -0.3 192,252 - BBR_RS10910 0.65 -0.5 192,330 + BBR_RS10910 0.72 -0.2 192,341 + BBR_RS10910 0.73 +1.0 192,341 + BBR_RS10910 0.73 -0.5 192,341 + BBR_RS10910 0.73 -0.3 192,341 + BBR_RS10910 0.73 +0.7 192,355 + BBR_RS10910 0.75 -0.2 192,360 + BBR_RS10910 0.75 +0.2 192,360 + BBR_RS10910 0.75 -1.1 192,360 + BBR_RS10910 0.75 +1.0 192,360 + BBR_RS10910 0.75 -0.8 192,364 + BBR_RS10910 0.76 +0.5 192,365 + BBR_RS10910 0.76 +0.2 192,368 - BBR_RS10910 0.76 +1.5 192,368 - BBR_RS10910 0.76 +0.3 192,368 - BBR_RS10910 0.76 +0.5 192,388 + BBR_RS10910 0.78 +0.9 192,388 + BBR_RS10910 0.78 +0.2 192,388 + BBR_RS10910 0.78 +0.4 192,388 + BBR_RS10910 0.78 +0.1 192,388 + BBR_RS10910 0.78 -0.1 192,388 + BBR_RS10910 0.78 +0.5 192,388 + BBR_RS10910 0.78 +0.4 192,400 + BBR_RS10910 0.79 -0.4
Or see this region's nucleotide sequence