Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10610 and BBR_RS10615 overlap by 4 nucleotides BBR_RS10615 and BBR_RS10620 overlap by 4 nucleotides
BBR_RS10610: BBR_RS10610 - penicillin-binding protein, at 98,056 to 99,522
_RS10610
BBR_RS10615: BBR_RS10615 - FtsW/RodA/SpoVE family cell cycle protein, at 99,519 to 101,126
_RS10615
BBR_RS10620: BBR_RS10620 - phosphoprotein phosphatase, at 101,123 to 102,742
_RS10620
Position (kb)
99
100
101
102 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 99.525 kb on - strand at 99.540 kb on - strand at 99.554 kb on - strand at 99.584 kb on + strand at 99.584 kb on + strand at 99.588 kb on - strand at 99.588 kb on - strand at 99.592 kb on + strand at 99.610 kb on - strand at 99.612 kb on - strand at 99.624 kb on - strand at 99.626 kb on + strand at 99.626 kb on + strand at 99.630 kb on + strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.650 kb on - strand at 99.661 kb on - strand at 99.661 kb on - strand at 99.671 kb on - strand at 99.697 kb on - strand, within BBR_RS10615 at 99.700 kb on - strand, within BBR_RS10615 at 99.725 kb on - strand, within BBR_RS10615 at 99.725 kb on - strand, within BBR_RS10615 at 99.748 kb on - strand, within BBR_RS10615 at 99.748 kb on - strand, within BBR_RS10615 at 99.769 kb on - strand, within BBR_RS10615 at 99.808 kb on - strand, within BBR_RS10615 at 101.178 kb on + strand at 101.238 kb on - strand at 101.245 kb on - strand at 101.325 kb on - strand, within BBR_RS10620 at 101.325 kb on - strand, within BBR_RS10620 at 101.325 kb on - strand, within BBR_RS10620 at 101.325 kb on - strand, within BBR_RS10620 at 101.412 kb on - strand, within BBR_RS10620 at 101.424 kb on - strand, within BBR_RS10620 at 101.424 kb on - strand, within BBR_RS10620 at 101.439 kb on - strand, within BBR_RS10620 at 101.489 kb on - strand, within BBR_RS10620 at 101.490 kb on - strand, within BBR_RS10620 at 101.491 kb on - strand, within BBR_RS10620 at 101.523 kb on + strand, within BBR_RS10620 at 101.536 kb on - strand, within BBR_RS10620
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 99,525 - -0.0 99,540 - -0.9 99,554 - -0.2 99,584 + +0.4 99,584 + -0.6 99,588 - -0.2 99,588 - -0.4 99,592 + -0.9 99,610 - -0.7 99,612 - -0.1 99,624 - +0.2 99,626 + -1.4 99,626 + -2.3 99,630 + -0.9 99,638 - +0.3 99,638 - -1.2 99,638 - -0.7 99,650 - -0.0 99,661 - -0.6 99,661 - -1.6 99,671 - -0.6 99,697 - BBR_RS10615 0.11 -0.4 99,700 - BBR_RS10615 0.11 -1.0 99,725 - BBR_RS10615 0.13 -0.3 99,725 - BBR_RS10615 0.13 -0.3 99,748 - BBR_RS10615 0.14 -1.0 99,748 - BBR_RS10615 0.14 -1.7 99,769 - BBR_RS10615 0.16 -0.3 99,808 - BBR_RS10615 0.18 +0.1 101,178 + -1.7 101,238 - -0.0 101,245 - -0.9 101,325 - BBR_RS10620 0.12 -1.2 101,325 - BBR_RS10620 0.12 -1.1 101,325 - BBR_RS10620 0.12 -2.0 101,325 - BBR_RS10620 0.12 -0.5 101,412 - BBR_RS10620 0.18 -2.0 101,424 - BBR_RS10620 0.19 -3.1 101,424 - BBR_RS10620 0.19 -4.2 101,439 - BBR_RS10620 0.20 -2.8 101,489 - BBR_RS10620 0.23 -0.9 101,490 - BBR_RS10620 0.23 -2.4 101,491 - BBR_RS10620 0.23 -0.9 101,523 + BBR_RS10620 0.25 +1.5 101,536 - BBR_RS10620 0.25 -2.8
Or see this region's nucleotide sequence