Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10550 and BBR_RS10555 are separated by 133 nucleotides BBR_RS10555 and BBR_RS10560 are separated by 212 nucleotides
BBR_RS10550: BBR_RS10550 - tryptophan--tRNA ligase, at 84,703 to 85,794
_RS10550
BBR_RS10555: BBR_RS10555 - DUF3073 domain-containing protein, at 85,928 to 86,311
_RS10555
BBR_RS10560: BBR_RS10560 - hypothetical protein, at 86,524 to 87,093
_RS10560
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-2
-1
0
1 at 85.777 kb on - strand at 85.810 kb on - strand at 85.810 kb on - strand at 85.867 kb on + strand at 85.873 kb on + strand at 85.875 kb on - strand at 85.875 kb on - strand at 85.879 kb on + strand at 85.881 kb on - strand at 85.904 kb on + strand at 85.920 kb on + strand at 86.052 kb on + strand, within BBR_RS10555 at 86.235 kb on + strand, within BBR_RS10555 at 86.275 kb on + strand at 86.275 kb on + strand at 86.283 kb on - strand at 86.296 kb on + strand at 86.296 kb on + strand at 86.296 kb on + strand at 86.315 kb on - strand at 86.315 kb on - strand at 86.317 kb on + strand at 86.325 kb on - strand at 86.351 kb on - strand at 86.352 kb on + strand at 86.361 kb on + strand at 86.361 kb on + strand at 86.361 kb on - strand at 86.369 kb on - strand at 86.373 kb on + strand at 86.401 kb on + strand at 86.401 kb on + strand at 86.401 kb on + strand at 86.425 kb on - strand at 86.425 kb on - strand at 86.440 kb on - strand at 86.447 kb on + strand at 86.475 kb on + strand at 86.493 kb on + strand at 86.520 kb on - strand at 86.530 kb on - strand at 86.535 kb on - strand at 86.536 kb on - strand at 86.559 kb on + strand at 86.567 kb on + strand at 86.572 kb on + strand at 86.575 kb on - strand at 86.580 kb on - strand at 86.580 kb on - strand at 86.586 kb on + strand, within BBR_RS10560 at 86.598 kb on + strand, within BBR_RS10560 at 86.598 kb on + strand, within BBR_RS10560 at 86.600 kb on + strand, within BBR_RS10560 at 86.606 kb on - strand, within BBR_RS10560 at 86.608 kb on - strand, within BBR_RS10560 at 86.612 kb on + strand, within BBR_RS10560 at 86.612 kb on + strand, within BBR_RS10560 at 86.617 kb on + strand, within BBR_RS10560 at 86.619 kb on - strand, within BBR_RS10560 at 86.655 kb on + strand, within BBR_RS10560 at 86.655 kb on + strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.664 kb on + strand, within BBR_RS10560 at 86.664 kb on - strand, within BBR_RS10560 at 86.665 kb on + strand, within BBR_RS10560 at 86.665 kb on + strand, within BBR_RS10560 at 86.669 kb on + strand, within BBR_RS10560 at 86.670 kb on + strand, within BBR_RS10560 at 86.672 kb on - strand, within BBR_RS10560 at 86.673 kb on - strand, within BBR_RS10560 at 86.678 kb on - strand, within BBR_RS10560 at 86.678 kb on - strand, within BBR_RS10560 at 86.685 kb on + strand, within BBR_RS10560 at 86.687 kb on - strand, within BBR_RS10560 at 86.719 kb on - strand, within BBR_RS10560 at 86.724 kb on - strand, within BBR_RS10560 at 86.728 kb on + strand, within BBR_RS10560 at 86.728 kb on + strand, within BBR_RS10560 at 86.733 kb on + strand, within BBR_RS10560 at 86.733 kb on + strand, within BBR_RS10560 at 86.734 kb on - strand, within BBR_RS10560 at 86.741 kb on - strand, within BBR_RS10560 at 86.747 kb on - strand, within BBR_RS10560 at 86.760 kb on + strand, within BBR_RS10560 at 86.760 kb on + strand, within BBR_RS10560 at 86.785 kb on - strand, within BBR_RS10560 at 86.785 kb on - strand, within BBR_RS10560 at 86.789 kb on + strand, within BBR_RS10560 at 86.794 kb on + strand, within BBR_RS10560 at 86.796 kb on + strand, within BBR_RS10560 at 86.797 kb on - strand, within BBR_RS10560 at 86.798 kb on - strand, within BBR_RS10560 at 86.809 kb on + strand, within BBR_RS10560 at 86.814 kb on + strand, within BBR_RS10560 at 86.816 kb on + strand, within BBR_RS10560 at 86.816 kb on + strand, within BBR_RS10560 at 86.817 kb on - strand, within BBR_RS10560 at 86.822 kb on - strand, within BBR_RS10560 at 86.824 kb on - strand, within BBR_RS10560 at 86.824 kb on - strand, within BBR_RS10560 at 86.827 kb on + strand, within BBR_RS10560 at 86.835 kb on - strand, within BBR_RS10560 at 86.835 kb on - strand, within BBR_RS10560 at 86.844 kb on - strand, within BBR_RS10560 at 86.852 kb on - strand, within BBR_RS10560 at 86.853 kb on + strand, within BBR_RS10560 at 86.861 kb on - strand, within BBR_RS10560 at 86.880 kb on + strand, within BBR_RS10560 at 86.880 kb on + strand, within BBR_RS10560 at 86.884 kb on - strand, within BBR_RS10560 at 86.905 kb on - strand, within BBR_RS10560 at 86.912 kb on + strand, within BBR_RS10560 at 86.938 kb on + strand, within BBR_RS10560 at 86.946 kb on - strand, within BBR_RS10560 at 86.948 kb on + strand, within BBR_RS10560 at 86.960 kb on + strand, within BBR_RS10560 at 86.978 kb on + strand, within BBR_RS10560 at 87.045 kb on - strand at 87.045 kb on - strand at 87.096 kb on + strand at 87.096 kb on + strand at 87.096 kb on + strand at 87.102 kb on + strand at 87.110 kb on - strand at 87.115 kb on - strand at 87.180 kb on - strand at 87.221 kb on + strand at 87.221 kb on + strand at 87.221 kb on + strand at 87.223 kb on + strand at 87.223 kb on + strand at 87.229 kb on - strand at 87.229 kb on - strand at 87.229 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.241 kb on - strand at 87.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 85,777 - -1.2 85,810 - -0.2 85,810 - -0.2 85,867 + +0.1 85,873 + -0.3 85,875 - +0.8 85,875 - -0.2 85,879 + -0.3 85,881 - -1.1 85,904 + -0.7 85,920 + -0.1 86,052 + BBR_RS10555 0.32 -2.0 86,235 + BBR_RS10555 0.80 -0.1 86,275 + -1.6 86,275 + -0.5 86,283 - +0.2 86,296 + -1.4 86,296 + -0.3 86,296 + -1.4 86,315 - -0.2 86,315 - +0.6 86,317 + -0.4 86,325 - +0.3 86,351 - +0.8 86,352 + -0.3 86,361 + -0.3 86,361 + +0.0 86,361 - +0.2 86,369 - -0.1 86,373 + -0.2 86,401 + +0.1 86,401 + +0.1 86,401 + -0.3 86,425 - +0.5 86,425 - +0.2 86,440 - -0.4 86,447 + +0.2 86,475 + -0.4 86,493 + -0.2 86,520 - -0.0 86,530 - -0.2 86,535 - +0.1 86,536 - -0.1 86,559 + -0.4 86,567 + -0.3 86,572 + +0.2 86,575 - -0.5 86,580 - -0.0 86,580 - +0.4 86,586 + BBR_RS10560 0.11 -0.7 86,598 + BBR_RS10560 0.13 +0.3 86,598 + BBR_RS10560 0.13 -0.7 86,600 + BBR_RS10560 0.13 +0.3 86,606 - BBR_RS10560 0.14 -0.7 86,608 - BBR_RS10560 0.15 -0.8 86,612 + BBR_RS10560 0.15 +0.2 86,612 + BBR_RS10560 0.15 +0.1 86,617 + BBR_RS10560 0.16 +0.7 86,619 - BBR_RS10560 0.17 -0.1 86,655 + BBR_RS10560 0.23 +0.2 86,655 + BBR_RS10560 0.23 +0.3 86,663 - BBR_RS10560 0.24 -0.6 86,663 - BBR_RS10560 0.24 +0.1 86,663 - BBR_RS10560 0.24 +0.9 86,664 + BBR_RS10560 0.25 +0.8 86,664 - BBR_RS10560 0.25 +0.9 86,665 + BBR_RS10560 0.25 -0.6 86,665 + BBR_RS10560 0.25 +0.5 86,669 + BBR_RS10560 0.25 -0.8 86,670 + BBR_RS10560 0.26 +0.2 86,672 - BBR_RS10560 0.26 +0.2 86,673 - BBR_RS10560 0.26 +0.4 86,678 - BBR_RS10560 0.27 -1.1 86,678 - BBR_RS10560 0.27 -0.7 86,685 + BBR_RS10560 0.28 +0.3 86,687 - BBR_RS10560 0.29 -0.2 86,719 - BBR_RS10560 0.34 -0.2 86,724 - BBR_RS10560 0.35 -0.5 86,728 + BBR_RS10560 0.36 +0.2 86,728 + BBR_RS10560 0.36 -0.9 86,733 + BBR_RS10560 0.37 +0.2 86,733 + BBR_RS10560 0.37 +0.5 86,734 - BBR_RS10560 0.37 -0.2 86,741 - BBR_RS10560 0.38 +0.6 86,747 - BBR_RS10560 0.39 -0.2 86,760 + BBR_RS10560 0.41 -0.8 86,760 + BBR_RS10560 0.41 +0.4 86,785 - BBR_RS10560 0.46 -0.2 86,785 - BBR_RS10560 0.46 +0.1 86,789 + BBR_RS10560 0.46 +0.9 86,794 + BBR_RS10560 0.47 -0.1 86,796 + BBR_RS10560 0.48 +0.6 86,797 - BBR_RS10560 0.48 -0.4 86,798 - BBR_RS10560 0.48 -0.3 86,809 + BBR_RS10560 0.50 -0.3 86,814 + BBR_RS10560 0.51 +0.1 86,816 + BBR_RS10560 0.51 +0.1 86,816 + BBR_RS10560 0.51 -0.3 86,817 - BBR_RS10560 0.51 -0.5 86,822 - BBR_RS10560 0.52 +0.2 86,824 - BBR_RS10560 0.53 +0.3 86,824 - BBR_RS10560 0.53 -0.3 86,827 + BBR_RS10560 0.53 +0.5 86,835 - BBR_RS10560 0.55 +1.5 86,835 - BBR_RS10560 0.55 -0.5 86,844 - BBR_RS10560 0.56 -0.7 86,852 - BBR_RS10560 0.58 +1.1 86,853 + BBR_RS10560 0.58 -0.0 86,861 - BBR_RS10560 0.59 -0.0 86,880 + BBR_RS10560 0.62 +0.9 86,880 + BBR_RS10560 0.62 -0.3 86,884 - BBR_RS10560 0.63 -0.5 86,905 - BBR_RS10560 0.67 -0.8 86,912 + BBR_RS10560 0.68 -0.6 86,938 + BBR_RS10560 0.73 -0.2 86,946 - BBR_RS10560 0.74 -0.7 86,948 + BBR_RS10560 0.74 +0.1 86,960 + BBR_RS10560 0.76 -0.6 86,978 + BBR_RS10560 0.80 +0.4 87,045 - -0.9 87,045 - +0.5 87,096 + -1.0 87,096 + -0.4 87,096 + -1.1 87,102 + +0.0 87,110 - +0.3 87,115 - +0.7 87,180 - -0.6 87,221 + +0.5 87,221 + +0.8 87,221 + +0.5 87,223 + +0.1 87,223 + +0.1 87,229 - -1.0 87,229 - -0.8 87,229 - -0.9 87,231 - -1.8 87,231 - -1.0 87,231 - -0.4 87,231 - -1.3 87,231 - -0.9 87,231 - -1.1 87,241 - -0.5 87,244 + +0.2
Or see this region's nucleotide sequence