Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10255 and BBR_RS20555 are separated by 65 nucleotides BBR_RS20555 and BBR_RS10260 are separated by 104 nucleotides
BBR_RS10255: BBR_RS10255 - chromosomal replication initiator protein DnaA, at 1 to 1,467
_RS10255
BBR_RS20555: BBR_RS20555 - hypothetical protein, at 1,533 to 1,938
_RS20555
BBR_RS10260: BBR_RS10260 - DNA polymerase III subunit beta, at 2,043 to 3,167
_RS10260
Position (kb) 0
1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 0.021 kb on + strand at 0.168 kb on + strand, within BBR_RS10255 at 0.168 kb on + strand, within BBR_RS10255 at 0.168 kb on + strand, within BBR_RS10255 at 0.238 kb on + strand, within BBR_RS10255 at 0.291 kb on + strand, within BBR_RS10255 at 1.462 kb on + strand at 1.466 kb on + strand at 1.466 kb on + strand at 1.466 kb on + strand at 1.466 kb on + strand at 1.466 kb on + strand at 1.466 kb on + strand at 1.466 kb on + strand at 1.466 kb on + strand at 1.470 kb on - strand at 1.472 kb on + strand at 1.474 kb on - strand at 1.474 kb on - strand at 1.474 kb on - strand at 1.474 kb on - strand at 1.474 kb on - strand at 1.474 kb on - strand at 1.480 kb on - strand at 1.480 kb on - strand at 1.480 kb on - strand at 1.510 kb on + strand at 1.518 kb on - strand at 1.518 kb on - strand at 1.578 kb on + strand, within BBR_RS20555 at 1.622 kb on + strand, within BBR_RS20555 at 1.630 kb on - strand, within BBR_RS20555 at 1.630 kb on - strand, within BBR_RS20555 at 1.966 kb on + strand at 2.031 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 21 + -1.6 168 + BBR_RS10255 0.11 -2.1 168 + BBR_RS10255 0.11 -1.5 168 + BBR_RS10255 0.11 -0.6 238 + BBR_RS10255 0.16 -1.9 291 + BBR_RS10255 0.20 -3.3 1,462 + -0.3 1,466 + -0.3 1,466 + +0.1 1,466 + +0.5 1,466 + -0.4 1,466 + -0.2 1,466 + +0.6 1,466 + +0.2 1,466 + -0.1 1,470 - +0.4 1,472 + +0.3 1,474 - +0.1 1,474 - +0.4 1,474 - +0.5 1,474 - -0.1 1,474 - +0.8 1,474 - +0.7 1,480 - -0.3 1,480 - -0.7 1,480 - -0.9 1,510 + +0.9 1,518 - -0.8 1,518 - +0.2 1,578 + BBR_RS20555 0.11 -0.6 1,622 + BBR_RS20555 0.22 +0.3 1,630 - BBR_RS20555 0.24 +0.6 1,630 - BBR_RS20555 0.24 -0.4 1,966 + -0.6 2,031 + -0.0
Or see this region's nucleotide sequence