Experiment: m.b. Vancomycin 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_R0044 and Dshi_2381 are separated by 190 nucleotides Dshi_2381 and Dshi_2382 are separated by 36 nucleotides Dshi_2382 and Dshi_2383 are separated by 411 nucleotides
Dshi_R0044: Dshi_R0044 - tRNA-Leu (RefSeq), at 2,515,206 to 2,515,292
_R0044
Dshi_2381: Dshi_2381 - lipoate-protein ligase B (RefSeq), at 2,515,483 to 2,516,145
_2381
Dshi_2382: Dshi_2382 - hypothetical protein (RefSeq), at 2,516,182 to 2,516,721
_2382
Dshi_2383: Dshi_2383 - Cytochrome c oxidase subunit I type (RefSeq), at 2,517,133 to 2,518,809
_2383
Position (kb)
2516
2517 Strain fitness (log2 ratio)
-1
0
1 at 2515.459 kb on + strand at 2516.140 kb on + strand at 2516.200 kb on - strand at 2516.219 kb on - strand at 2516.243 kb on - strand, within Dshi_2382 at 2516.249 kb on + strand, within Dshi_2382 at 2516.257 kb on - strand, within Dshi_2382 at 2516.285 kb on - strand, within Dshi_2382 at 2516.668 kb on - strand at 2516.760 kb on + strand at 2516.835 kb on + strand at 2516.958 kb on - strand at 2517.137 kb on - strand at 2517.280 kb on + strand at 2517.395 kb on - strand, within Dshi_2383 at 2517.678 kb on - strand, within Dshi_2383
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Vancomycin 0.064 mg/ml remove 2,515,459 + +0.0 2,516,140 + -0.5 2,516,200 - +0.3 2,516,219 - -0.1 2,516,243 - Dshi_2382 0.11 +0.2 2,516,249 + Dshi_2382 0.12 -0.6 2,516,257 - Dshi_2382 0.14 +0.1 2,516,285 - Dshi_2382 0.19 -0.2 2,516,668 - +0.2 2,516,760 + +0.2 2,516,835 + -0.8 2,516,958 - +0.1 2,517,137 - -1.3 2,517,280 + +0.5 2,517,395 - Dshi_2383 0.16 -0.6 2,517,678 - Dshi_2383 0.32 -0.2
Or see this region's nucleotide sequence