Experiment: m.b. Vancomycin 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0187 and Dshi_0188 are separated by 662 nucleotides Dshi_0188 and Dshi_0189 are separated by 153 nucleotides Dshi_0189 and Dshi_0190 are separated by 3 nucleotides
Dshi_0187: Dshi_0187 - Lysine exporter protein (LYSE/YGGA) (RefSeq), at 188,367 to 188,981
_0187
Dshi_0188: Dshi_0188 - hydroxymethylbutenyl pyrophosphate reductase (RefSeq), at 189,644 to 190,600
_0188
Dshi_0189: Dshi_0189 - cytochrome c class I (RefSeq), at 190,754 to 191,188
_0189
Dshi_0190: Dshi_0190 - membrane protein-like protein (RefSeq), at 191,192 to 191,707
_0190
Position (kb)
189
190
191 Strain fitness (log2 ratio)
-2
-1
0
1 at 188.818 kb on - strand, within Dshi_0187 at 188.911 kb on + strand, within Dshi_0187 at 188.918 kb on - strand, within Dshi_0187 at 189.014 kb on - strand at 189.044 kb on + strand at 189.052 kb on - strand at 189.075 kb on - strand at 189.145 kb on + strand at 189.152 kb on + strand at 189.160 kb on - strand at 189.169 kb on - strand at 189.172 kb on - strand at 189.172 kb on - strand at 189.172 kb on - strand at 189.179 kb on + strand at 189.218 kb on - strand at 189.413 kb on + strand at 189.554 kb on - strand at 189.570 kb on + strand at 189.578 kb on - strand at 189.578 kb on - strand at 189.588 kb on - strand at 189.618 kb on - strand at 190.727 kb on + strand at 190.728 kb on - strand at 190.738 kb on + strand at 190.786 kb on + strand at 190.792 kb on + strand at 190.822 kb on + strand, within Dshi_0189 at 190.862 kb on + strand, within Dshi_0189 at 190.869 kb on - strand, within Dshi_0189 at 190.891 kb on - strand, within Dshi_0189 at 190.893 kb on + strand, within Dshi_0189 at 190.901 kb on - strand, within Dshi_0189 at 191.061 kb on - strand, within Dshi_0189 at 191.130 kb on - strand, within Dshi_0189 at 191.146 kb on + strand at 191.156 kb on + strand at 191.175 kb on - strand at 191.214 kb on + strand at 191.221 kb on - strand at 191.222 kb on - strand at 191.327 kb on + strand, within Dshi_0190 at 191.327 kb on + strand, within Dshi_0190 at 191.327 kb on + strand, within Dshi_0190 at 191.335 kb on - strand, within Dshi_0190 at 191.342 kb on + strand, within Dshi_0190 at 191.389 kb on - strand, within Dshi_0190 at 191.431 kb on - strand, within Dshi_0190 at 191.460 kb on + strand, within Dshi_0190 at 191.465 kb on + strand, within Dshi_0190 at 191.465 kb on + strand, within Dshi_0190 at 191.473 kb on - strand, within Dshi_0190 at 191.473 kb on - strand, within Dshi_0190 at 191.473 kb on - strand, within Dshi_0190 at 191.532 kb on + strand, within Dshi_0190 at 191.539 kb on + strand, within Dshi_0190 at 191.547 kb on - strand, within Dshi_0190
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Vancomycin 0.064 mg/ml remove 188,818 - Dshi_0187 0.73 -0.8 188,911 + Dshi_0187 0.88 -1.0 188,918 - Dshi_0187 0.90 -0.4 189,014 - +0.1 189,044 + -0.3 189,052 - -0.7 189,075 - +0.1 189,145 + -0.6 189,152 + -0.5 189,160 - -1.0 189,169 - -0.6 189,172 - -0.1 189,172 - +0.0 189,172 - +0.7 189,179 + -1.0 189,218 - -0.1 189,413 + +1.4 189,554 - -0.4 189,570 + -0.4 189,578 - +0.4 189,578 - -0.6 189,588 - +0.6 189,618 - +0.4 190,727 + +0.7 190,728 - -0.3 190,738 + +1.4 190,786 + +1.4 190,792 + +0.1 190,822 + Dshi_0189 0.16 -0.9 190,862 + Dshi_0189 0.25 -0.5 190,869 - Dshi_0189 0.26 -0.1 190,891 - Dshi_0189 0.31 -0.7 190,893 + Dshi_0189 0.32 -1.1 190,901 - Dshi_0189 0.34 -0.2 191,061 - Dshi_0189 0.71 +0.4 191,130 - Dshi_0189 0.86 -1.9 191,146 + +0.1 191,156 + -1.3 191,175 - +0.1 191,214 + -0.9 191,221 - -0.6 191,222 - -0.9 191,327 + Dshi_0190 0.26 -0.1 191,327 + Dshi_0190 0.26 -0.8 191,327 + Dshi_0190 0.26 -0.1 191,335 - Dshi_0190 0.28 +0.1 191,342 + Dshi_0190 0.29 +0.9 191,389 - Dshi_0190 0.38 +0.3 191,431 - Dshi_0190 0.46 -0.6 191,460 + Dshi_0190 0.52 -1.1 191,465 + Dshi_0190 0.53 -0.8 191,465 + Dshi_0190 0.53 -1.1 191,473 - Dshi_0190 0.54 -0.1 191,473 - Dshi_0190 0.54 +1.1 191,473 - Dshi_0190 0.54 -0.6 191,532 + Dshi_0190 0.66 -0.9 191,539 + Dshi_0190 0.67 -0.1 191,547 - Dshi_0190 0.69 -1.1
Or see this region's nucleotide sequence