Experiment: Pyruvate-Thiosulfate (60-15 mM) + 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt maF and pgpA overlap by 8 nucleotides pgpA and rrf2 are separated by 100 nucleotides rrf2 and rrf1 are separated by 3 nucleotides rrf1 and hmcF are separated by 92 nucleotides
DVU0527: maF - septum formation protein Maf (TIGR), at 600,433 to 601,065
maF
DVU0528: pgpA - phosphatidylglycerophosphatase (TIGR), at 601,058 to 601,537
pgpA
DVU0529: rrf2 - Rrf2 protein (voordouw), at 601,638 to 602,090
rrf2
DVU0530: rrf1 - Rrf1 protein (voordouw), at 602,094 to 602,510
rrf1
DVU0531: hmcF - HmcF, 52.7 kd protein in hmc operon (voordouw), at 602,603 to 603,988
hmcF
Position (kb) 601
602
603 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 600.642 kb on + strand, within maF at 600.648 kb on - strand, within maF at 600.677 kb on - strand, within maF at 600.692 kb on + strand, within maF at 600.726 kb on + strand, within maF at 600.775 kb on + strand, within maF at 600.822 kb on - strand, within maF at 600.932 kb on - strand, within maF at 601.521 kb on + strand at 601.580 kb on - strand at 601.629 kb on + strand at 601.636 kb on + strand at 601.776 kb on + strand, within rrf2 at 601.791 kb on - strand, within rrf2 at 601.851 kb on + strand, within rrf2 at 601.872 kb on + strand, within rrf2 at 601.872 kb on + strand, within rrf2 at 601.936 kb on + strand, within rrf2 at 602.011 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.085 kb on + strand at 602.087 kb on - strand at 602.143 kb on - strand, within rrf1 at 602.200 kb on - strand, within rrf1 at 602.259 kb on + strand, within rrf1 at 602.353 kb on - strand, within rrf1 at 602.398 kb on - strand, within rrf1 at 602.425 kb on + strand, within rrf1 at 602.440 kb on + strand, within rrf1 at 602.448 kb on - strand, within rrf1 at 602.508 kb on + strand at 602.518 kb on - strand at 602.524 kb on - strand at 602.624 kb on - strand at 602.726 kb on + strand at 602.804 kb on - strand, within hmcF at 603.048 kb on + strand, within hmcF at 603.048 kb on + strand, within hmcF at 603.090 kb on - strand, within hmcF
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Thiosulfate (60-15 mM) + 0.1% Yeast Extract remove 600,642 + maF DVU0527 0.33 -0.5 600,648 - maF DVU0527 0.34 -0.3 600,677 - maF DVU0527 0.39 -0.1 600,692 + maF DVU0527 0.41 +0.0 600,726 + maF DVU0527 0.46 +0.1 600,775 + maF DVU0527 0.54 -0.7 600,822 - maF DVU0527 0.61 +1.2 600,932 - maF DVU0527 0.79 +0.4 601,521 + -0.1 601,580 - +0.1 601,629 + -2.0 601,636 + -0.4 601,776 + rrf2 DVU0529 0.30 +0.6 601,791 - rrf2 DVU0529 0.34 +0.8 601,851 + rrf2 DVU0529 0.47 +1.3 601,872 + rrf2 DVU0529 0.52 +0.0 601,872 + rrf2 DVU0529 0.52 +0.3 601,936 + rrf2 DVU0529 0.66 -0.3 602,011 + rrf2 DVU0529 0.82 -0.3 602,021 + rrf2 DVU0529 0.85 +0.9 602,021 + rrf2 DVU0529 0.85 +0.8 602,021 + rrf2 DVU0529 0.85 +1.2 602,085 + +0.3 602,087 - -0.4 602,143 - rrf1 DVU0530 0.12 +0.6 602,200 - rrf1 DVU0530 0.25 -1.0 602,259 + rrf1 DVU0530 0.40 +1.2 602,353 - rrf1 DVU0530 0.62 -0.4 602,398 - rrf1 DVU0530 0.73 +0.0 602,425 + rrf1 DVU0530 0.79 +0.2 602,440 + rrf1 DVU0530 0.83 +1.6 602,448 - rrf1 DVU0530 0.85 -2.6 602,508 + -0.4 602,518 - +0.1 602,524 - +0.2 602,624 - -5.0 602,726 + -0.9 602,804 - hmcF DVU0531 0.15 -1.0 603,048 + hmcF DVU0531 0.32 -0.1 603,048 + hmcF DVU0531 0.32 -0.1 603,090 - hmcF DVU0531 0.35 -0.4
Or see this region's nucleotide sequence