Experiment: pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_531 and HP15_532 overlap by 28 nucleotides HP15_532 and HP15_533 are separated by 64 nucleotides
HP15_531: HP15_531 - acetolactate synthase, large subunit, biosynthetic type, at 548,178 to 549,896
_531
HP15_532: HP15_532 - acetolactate synthase 3 regulatory subunit, at 549,869 to 550,390
_532
HP15_533: HP15_533 - ketol-acid reductoisomerase, at 550,455 to 551,471
_533
Position (kb) 549
550
551 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 548.890 kb on + strand, within HP15_531 at 548.900 kb on + strand, within HP15_531 at 549.000 kb on - strand, within HP15_531 at 549.016 kb on - strand, within HP15_531 at 549.158 kb on + strand, within HP15_531 at 549.181 kb on - strand, within HP15_531 at 549.472 kb on + strand, within HP15_531 at 549.509 kb on - strand, within HP15_531 at 549.611 kb on + strand, within HP15_531 at 549.662 kb on + strand, within HP15_531 at 549.827 kb on + strand at 549.881 kb on + strand at 550.256 kb on - strand, within HP15_532 at 550.256 kb on - strand, within HP15_532 at 550.267 kb on + strand, within HP15_532 at 550.296 kb on + strand, within HP15_532 at 550.296 kb on + strand, within HP15_532 at 550.296 kb on + strand, within HP15_532 at 550.383 kb on + strand at 550.393 kb on + strand at 550.404 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate remove 548,890 + HP15_531 0.41 -3.9 548,900 + HP15_531 0.42 -0.2 549,000 - HP15_531 0.48 +0.8 549,016 - HP15_531 0.49 -2.7 549,158 + HP15_531 0.57 -0.8 549,181 - HP15_531 0.58 -3.6 549,472 + HP15_531 0.75 -2.4 549,509 - HP15_531 0.77 -1.8 549,611 + HP15_531 0.83 -3.5 549,662 + HP15_531 0.86 -1.2 549,827 + -2.1 549,881 + -1.2 550,256 - HP15_532 0.74 -2.2 550,256 - HP15_532 0.74 -0.2 550,267 + HP15_532 0.76 -4.5 550,296 + HP15_532 0.82 -1.2 550,296 + HP15_532 0.82 -3.4 550,296 + HP15_532 0.82 -3.8 550,383 + -0.8 550,393 + -1.2 550,404 + -0.7
Or see this region's nucleotide sequence