Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26210 and BWI76_RS26215 overlap by 4 nucleotides BWI76_RS26215 and BWI76_RS26220 are separated by 247 nucleotides
BWI76_RS26210: BWI76_RS26210 - glycogen debranching enzyme, at 5,422,264 to 5,424,240
_RS26210
BWI76_RS26215: BWI76_RS26215 - glycogen-branching enzyme, at 5,424,237 to 5,426,423
_RS26215
BWI76_RS26220: BWI76_RS26220 - aspartate-semialdehyde dehydrogenase, at 5,426,671 to 5,427,777
_RS26220
Position (kb)
5424
5425
5426
5427 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5423.454 kb on - strand, within BWI76_RS26210 at 5423.763 kb on - strand, within BWI76_RS26210 at 5423.770 kb on - strand, within BWI76_RS26210 at 5423.802 kb on - strand, within BWI76_RS26210 at 5423.915 kb on + strand, within BWI76_RS26210 at 5423.978 kb on - strand, within BWI76_RS26210 at 5424.138 kb on + strand at 5424.183 kb on - strand at 5424.345 kb on - strand at 5424.504 kb on + strand, within BWI76_RS26215 at 5424.604 kb on - strand, within BWI76_RS26215 at 5424.652 kb on + strand, within BWI76_RS26215 at 5424.675 kb on + strand, within BWI76_RS26215 at 5424.786 kb on + strand, within BWI76_RS26215 at 5425.243 kb on + strand, within BWI76_RS26215 at 5425.243 kb on + strand, within BWI76_RS26215 at 5425.251 kb on - strand, within BWI76_RS26215 at 5425.317 kb on + strand, within BWI76_RS26215 at 5425.356 kb on - strand, within BWI76_RS26215 at 5425.578 kb on + strand, within BWI76_RS26215 at 5425.943 kb on + strand, within BWI76_RS26215 at 5426.125 kb on - strand, within BWI76_RS26215 at 5426.334 kb on + strand at 5426.413 kb on + strand at 5426.489 kb on - strand at 5426.501 kb on - strand at 5426.513 kb on - strand at 5426.535 kb on - strand at 5426.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 5,423,454 - BWI76_RS26210 0.60 +0.6 5,423,763 - BWI76_RS26210 0.76 +0.5 5,423,770 - BWI76_RS26210 0.76 +0.2 5,423,802 - BWI76_RS26210 0.78 +0.8 5,423,915 + BWI76_RS26210 0.84 -0.3 5,423,978 - BWI76_RS26210 0.87 -0.8 5,424,138 + -0.3 5,424,183 - -0.6 5,424,345 - -2.8 5,424,504 + BWI76_RS26215 0.12 -0.7 5,424,604 - BWI76_RS26215 0.17 -0.6 5,424,652 + BWI76_RS26215 0.19 -1.7 5,424,675 + BWI76_RS26215 0.20 -1.0 5,424,786 + BWI76_RS26215 0.25 +0.2 5,425,243 + BWI76_RS26215 0.46 +0.3 5,425,243 + BWI76_RS26215 0.46 -1.4 5,425,251 - BWI76_RS26215 0.46 -0.6 5,425,317 + BWI76_RS26215 0.49 -0.5 5,425,356 - BWI76_RS26215 0.51 -0.7 5,425,578 + BWI76_RS26215 0.61 -1.8 5,425,943 + BWI76_RS26215 0.78 -3.3 5,426,125 - BWI76_RS26215 0.86 -2.9 5,426,334 + -0.6 5,426,413 + -1.6 5,426,489 - +0.3 5,426,501 - -0.2 5,426,513 - -1.1 5,426,535 - -0.2 5,426,572 + -0.8
Or see this region's nucleotide sequence